Establishment of analyzing method to delineate the central nervous system active in rat with treadmill running and its application to exercise physiology.

跑步机跑步大鼠中枢神经系统活性分析方法的建立及其在运动生理学中的应用

基本信息

批准号：
08558005
负责人：
SOYA Hideaki
金额：
$ 3.58万
依托单位：
MIE UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1996
资助国家：
日本
起止时间：
1996 至 1997
项目状态：
已结题

项目摘要

We aimed to delineate the active site in rat brain running at the speed @ lactate threshold (LT). We therefore use our running model in which in male rat on the treadmill. During and after the LT running, whole brain was removed and sectioned frontal for an in situ hybridization for c-fos (Exp.1). Moreover, we further examined how the brain could express the c-fos after muscle contraction induced by an electrical atimulation of the sciatic nerve at the condition with *20 motorthreshold. In the expreiment, we examined c-fos protein by an immunohistochemistry. In the experiment, we should also examined the immunoreactivity of arginine-vasopressin (AVP) in the c-fos expressing cells. In the Exp.I,the c-fos mRNA was shown to be increasedin the loci such as hypothalamus (paraventricular nucleus and supraoptic nucleus) thalamus, hippocampus, and limbic area as well as motor center as parietal region. In the Exp.II,the positive staining with c-fos was found in the AVP cell, suggesting that AVP neuron will be involved in the running stress. The c-fos protein expressed within the neuronal cells expressing AVP was only observed in the condition that "exercise pressure response" was occurred. These results suggest that several loci in the rat brain should be active during the LT running. Especially, hypothalamic AVP neuron would be excited reflexively in the running. This would propose that running exercise at the moderate intensity could stimulate several region in the central nervous system especially in hypothalamus.Finally, we tried to examine how the MN innervating m.soleus (MN-SOL) and m.plantaris (MN-PL) could be recruited in the LT running by use of in situ hybridization for c-fos. Although the extent of c-fos expression in the MN-SOL was unchanged, that of MN-PL was increased just above the LT running, suggesting that these methods to detect c-fos gene/protein are available for exploring the active loci in the central nervous system in rat with running.

我们的目的是以速度 @乳酸阈值（LT）的速度划定大鼠大脑中的活动位点。因此，我们使用跑步机上雄性大鼠的跑步模型。在LT运行期间和之后，将整个大脑去除并截面，以进行C-FOS的原位杂交（Exp.1）。此外，我们进一步研究了大脑如何通过 *20 Motorthreshold的坐骨神经进行电化后的肌肉收缩后表达C-Fos。在大声疾呼中，我们通过免疫组织化学检查了C-FOS蛋白。在实验中，我们还应该检查C-Fos表达细胞中精氨酸 - 瓦抗蛋白（AVP）的免疫反应性。在EXP.I中，C-FOS mRNA显示出在基因座中增加的基因座，例如下丘脑（室室核和上核上核）丘脑，海马和边缘区和山羊区以及运动中心和顶部区域。在exp.ii中，在AVP细胞中发现了用C-FOS阳性染色，这表明AVP神经元将参与运行应力。仅在发生“运动压力反应”的情况下观察到在表达AVP的神经元细胞中表达的C-FOS蛋白。这些结果表明，在LT运行期间，大鼠大脑中的几个基因座应处于活动状态。尤其是，下丘脑AVP神经元在跑步中会反思。这将表明，以中等强度进行运动可以刺激中枢神经系统中的几个区域，特别是在下丘脑中。我们试图检查如何通过使用现有的C-FOS来招募LT来招募MN的MN支配M.Soleus（MN-SOL）和M. plantaris（MN-PL）。尽管MN-SOL中C-FOS表达的程度没有变化，但MN-PL的表达恰好在LT运行范围之上增加，这表明这些检测C-FOS基因/蛋白质的方法可用于探索与Rancor的大鼠中心神经系统中的活动基因座。