Establishment of analyzing method to delineate the central nervous system active in rat with treadmill running and its application to exercise physiology.

跑步机跑步大鼠中枢神经系统活性分析方法的建立及其在运动生理学中的应用

基本信息

批准号：
08558005
负责人：
SOYA Hideaki
金额：
$ 3.58万
依托单位：
MIE UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1996
资助国家：
日本
起止时间：
1996 至 1997
项目状态：
已结题

项目摘要

We aimed to delineate the active site in rat brain running at the speed @ lactate threshold (LT). We therefore use our running model in which in male rat on the treadmill. During and after the LT running, whole brain was removed and sectioned frontal for an in situ hybridization for c-fos (Exp.1). Moreover, we further examined how the brain could express the c-fos after muscle contraction induced by an electrical atimulation of the sciatic nerve at the condition with *20 motorthreshold. In the expreiment, we examined c-fos protein by an immunohistochemistry. In the experiment, we should also examined the immunoreactivity of arginine-vasopressin (AVP) in the c-fos expressing cells. In the Exp.I,the c-fos mRNA was shown to be increasedin the loci such as hypothalamus (paraventricular nucleus and supraoptic nucleus) thalamus, hippocampus, and limbic area as well as motor center as parietal region. In the Exp.II,the positive staining with c-fos was found in the AVP cell, suggesting that AVP neuron will be involved in the running stress. The c-fos protein expressed within the neuronal cells expressing AVP was only observed in the condition that "exercise pressure response" was occurred. These results suggest that several loci in the rat brain should be active during the LT running. Especially, hypothalamic AVP neuron would be excited reflexively in the running. This would propose that running exercise at the moderate intensity could stimulate several region in the central nervous system especially in hypothalamus.Finally, we tried to examine how the MN innervating m.soleus (MN-SOL) and m.plantaris (MN-PL) could be recruited in the LT running by use of in situ hybridization for c-fos. Although the extent of c-fos expression in the MN-SOL was unchanged, that of MN-PL was increased just above the LT running, suggesting that these methods to detect c-fos gene/protein are available for exploring the active loci in the central nervous system in rat with running.

我们的目的是描绘以乳酸阈值 (LT) 速度运行的大鼠大脑中的活性部位。因此，我们使用我们的跑步模型，其中雄性大鼠在跑步机上。在 LT 运行期间和之后，取出整个大脑并进行额叶切片，用于 c-fos 的原位杂交（实验 1）。此外，我们进一步研究了在*20运动阈值条件下，坐骨神经电刺激引起肌肉收缩后，大脑如何表达c-fos。在实验中，我们通过免疫组织化学检查了 c-fos 蛋白。在实验中，我们还应该检查c-fos表达细胞中精氨酸加压素（AVP）的免疫反应性。在实验I中，c-fos mRNA在下丘脑（室旁核和视上核）、丘脑、海马、边缘区以及顶叶区运动中枢等位点显示增加。实验II中，AVP细胞内发现c-fos阳性染色，提示AVP神经元可能参与跑步应激。仅在发生“运动压力反应”的情况下才观察到表达AVP的神经元细胞内表达的c-fos蛋白。这些结果表明，在 LT 跑步过程中，大鼠大脑中的几个位点应该处于活跃状态。尤其是下丘脑AVP神经元在跑步时会反射性兴奋。这表明中等强度的跑步运动可以刺激中枢神经系统的多个区域，尤其是下丘脑。最后，我们尝试研究 MN 如何支配比目鱼肌 (MN-SOL) 和跖肌 (MN-PL)可以通过使用 c-fos 原位杂交在 LT 运行中招募。尽管 MN-SOL 中 c-fos 表达的程度没有变化，但 MN-PL 的表达程度略高于 LT 运行，表明这些检测 c-fos 基因/蛋白质的方法可用于探索 MN-PL 中的活性位点。跑步时大鼠的中枢神经系统。