Physical mapping and molecular genetic study of barley chromosomes by genetic genome rearrangement

通过遗传基因组重排对大麦染色体进行物理作图和分子遗传学研究

基本信息

批准号：
08406001
负责人：
ENDO Takashi
金额：
$ 18.3万
依托单位：
KYOTO UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (A)
财政年份：
1996
资助国家：
日本
起止时间：
1996 至 1999
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08406001/
关键词：
barley common wheat gametocidal chromosome chromosomal structural changes physical chromosome map deletion mapping FISH GISH 染色体地図欠失転座 in situ hybridization サブテロメリック配列欠失系統 PCR

项目摘要

Aiming to construct physical maps of barley chromosomes based on various DNA markers, we conducted the present study to induce structural changes in barley chromosomes added to common wheat by the use of gametocidal chromosomes. The results obtained during the research period between 1996 and 2000 are as follows.1. Production of Barley Addition Lines Generating Chromosomal Structural ChangesWe introduced two gametocidal chromosomes, one from Aegilops cylindrica, one from Ae. triuncialis, into barley chromosome addition lines of common wheat by crossing and cytological screening.2. Development of Technique Identifying Barley Chromatin Based on Chromosome Banding and Fluorescence In Situ HybridizationCombining chromosome banding (C-banding and N-banding) and in situ hybridization (FISH using a probe of the barley-specific subtelomeric repeat and GISH using a probe of the total barley genomic DNA), we developed a technique by which we can identify barley chromatin in the genomic background of common wheat.3. Identification and Isolation of Barley Chromosomal Structural ChangesUsing the lines and technique mentioned above, we demonstrated the gametocidal chromosomes can induce structural changes in every barley chromosome with high frequencies.4. Physical Mapping of a Barley ChromosomeWe screened many deletions and translocations in barley chromosome 7H and conducted deletion mapping with them based on RFLP and AFLP markers, demonstrating the efficacy of the barley chromosomal structural changes generated in this study.

为了构建基于各种 DNA 标记的大麦染色体物理图谱，我们进行了本研究，通过使用杀配子染色体来诱导添加到普通小麦中的大麦染色体的结构变化。 1996年至2000年的研究取得的成果如下： 1．产生染色体结构变化的大麦附加系的生产我们引入了两种杀配子染色体，一种来自圆柱山羊草，一种来自伊蚊。通过杂交和细胞学筛选，将triuncialis转化为普通小麦的大麦染色体附加系。 2．基于染色体显带和荧光原位杂交的大麦染色质鉴定技术的开发结合了染色体显带（C带和N带）和原位杂交（使用大麦特异性亚端粒重复探针的FISH和使用总端粒重复探针的GISH）大麦基因组DNA），我们开发了一种技术，可以在普通小麦的基因组背景中识别大麦染色质。3。大麦染色体结构变化的鉴定和分离利用上述的细胞系和技术，我们证明了杀配子染色体可以高频率地诱导大麦每条染色体的结构变化。 4．大麦染色体的物理作图我们筛选了大麦7H染色体中的许多缺失和易位，并基于RFLP和AFLP标记对其进行了缺失作图，证明了本研究中产生的大麦染色体结构变化的功效。