A STUDY ON THE MECHANISM UNDERLYING MILK EJECTION BURSTS OF OXYTOCIN CELLS.

催产素细胞喷乳爆发机制的研究。

• 批准号: 07680869
• 负责人: NEGORO Hideo
• 金额: $ 1.22万
• 依托单位: Fukui Medical School
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07680869/
• 关键词: oxytocin; milk ejectin reflex; burst discharge; synchronization; afferent pathway; summatin mechanism; gate

In order to study the neural mechanisms to generate burst activities of oxytocin neurones during suckling in lactating rats, the following three studies were made :(1) the effect of midbrain-hemitransection on amplitudes and time courses of milk ejection bursts of oxytocin neurones in lactating rats.Extracellular recordings of the electrical activity of oxytocin neurones were made from the supraoptic nuclei of lactating rats, and the milk-ejection bursts were investigated in the midbrain-hemitransected rats. Of the burst recorded from oxytocin neurones on the lesioned side, the amplitude was smaller, the onset time was later and the burst span was shorter than those recorded on the non-lesion-ed side. These results indicated that the amplitude and time course of the burst of oxytocin neurones mainly depend on suckling information from the nipples on their opposite side.(2) the effects of unilateral suckling on milk ejection bursts of oxytocin neurones in intact rats.When application of pups was limited to the nipples on either the same side or the side opposite to the oxytocine neurone recorded, the burst amplitude was significantly lower than during bilateral suckling. Furthermore, the burst amplitude was significantly lower during ipsilateral suckling than during contralateral suckling. The finding indicates that the burst amplitude mainly depends on the amount of afferent suckling signals arising from the nipplies on the side opposite to the reocording side.(3) the effect of interhemispheric sections of the hypothalamus on milk-ejection bursts of supraoptic oxytocin neurones.Extensive interhemispheric sectioning did not abolish the burst of oxytocin neurones during bilateral and contralateral suckling but reduced the number of oxytocine neurones that showed the burst during ipsilateral suckling. The result suggest that there are two separate gate and summation mechanisms on each side of the hypothalamus.

In order to study the neural mechanisms to generate burst activities of oxytocin neurones during suckling in lactating rats, the following three studies were made :(1) the effect of midbrain-hemitransection on amplitudes and time courses of milk ejection bursts of oxytocin neurones in lactating rats.Extracellular recordings of the electrical activity of oxytocin neurones were made from the supraoptic在中脑左右横流的大鼠中研究了泌乳大鼠的核和牛奶批量爆发。从病变侧的催产素神经元记录的爆发中，振幅较小，发作时间较晚，而爆发跨度比在非乳胶侧记录的爆发时间短。这些结果表明，催产素神经元爆发的振幅和时间过程主要取决于其相对侧的乳头中的哺乳信息。（2）单侧哺乳对牛奶射精对催产蛋白神经元的牛奶射出爆发的影响，在幼虫的应用中，较低的乳头上的乳头限制了较低的乳头和乳头，这是相反的neipl and neipl and the neipl and the neipl and the Oxy的牛肉，或者是牛的。比双边哺乳期。此外，同侧哺乳期间的爆发幅度明显低于对侧哺乳期间的爆发幅度。发现表明，爆发幅度主要取决于与重新定位一侧相对的nipplies产生的传入哺乳信号的数量。（3）下丘脑的半球间截面的影响对牛奶偏用的牛奶偏用爆发对超氧蛋白神经蛋白神经蛋白神经蛋白的爆发的影响。双侧和对侧哺乳减少了催产量神经元的数量，这些神经元在同侧哺乳过程中表现出爆发。结果表明，下丘脑每一侧都有两个独立的门和求和机制。

项目成果

Wang,Y-F.,Negoro,H.,Honda,K.: "Effects of hemitransection of the medbrain on milk-ejection burst of oxytocin neurones in lactating rat." Journal of Endocrinology. 144. 463-470 (1995)

Wang，Y-F.，Negoro，H.，Honda，K.：“中脑半横切对哺乳大鼠催产素神经元乳汁喷射爆发的影响”。

Wang, Y-F., Negoro, H., Honda, K.: "Effects of hemitransection of the medbrain on milk-ejection burst of oxytocin neurones in lactating rat." J. Endocrinology. 144. 463-470 (1995)

Wang, Y-F.、Negoro, H.、Honda, K.：“中脑半横断对哺乳大鼠催产素神经元喷乳爆发的影响”。