A STUDY ON THE MECHANISM UNDERLYING MILK EJECTION BURSTS OF OXYTOCIN CELLS.

催产素细胞喷乳爆发机制的研究。

• 批准号: 07680869
• 负责人: NEGORO Hideo
• 金额: $ 1.22万
• 依托单位: Fukui Medical School
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07680869/
• 关键词: oxytocin; milk ejectin reflex; burst discharge; synchronization; afferent pathway; summatin mechanism; gate

In order to study the neural mechanisms to generate burst activities of oxytocin neurones during suckling in lactating rats, the following three studies were made :(1) the effect of midbrain-hemitransection on amplitudes and time courses of milk ejection bursts of oxytocin neurones in lactating rats.Extracellular recordings of the electrical activity of oxytocin neurones were made from the supraoptic nuclei of lactating rats, and the milk-ejection bursts were investigated in the midbrain-hemitransected rats. Of the burst recorded from oxytocin neurones on the lesioned side, the amplitude was smaller, the onset time was later and the burst span was shorter than those recorded on the non-lesion-ed side. These results indicated that the amplitude and time course of the burst of oxytocin neurones mainly depend on suckling information from the nipples on their opposite side.(2) the effects of unilateral suckling on milk ejection bursts of oxytocin neurones in intact rats.When application of pups was limited to the nipples on either the same side or the side opposite to the oxytocine neurone recorded, the burst amplitude was significantly lower than during bilateral suckling. Furthermore, the burst amplitude was significantly lower during ipsilateral suckling than during contralateral suckling. The finding indicates that the burst amplitude mainly depends on the amount of afferent suckling signals arising from the nipplies on the side opposite to the reocording side.(3) the effect of interhemispheric sections of the hypothalamus on milk-ejection bursts of supraoptic oxytocin neurones.Extensive interhemispheric sectioning did not abolish the burst of oxytocin neurones during bilateral and contralateral suckling but reduced the number of oxytocine neurones that showed the burst during ipsilateral suckling. The result suggest that there are two separate gate and summation mechanisms on each side of the hypothalamus.

为了研究哺乳后哺乳过程中催化蛋白神经元爆发活性的神经机制，进行了以下三项研究：（1）中脑 - 脱位对牛奶射精神经元在乳酸蛋白神经元的振幅和时间疗程的影响和时间疗程大鼠催产素神经元的电活性的细胞记录是由泌乳大鼠的上核核制成的，并研究了中脑 - 脱脂大鼠的牛奶注射爆发。从病变侧的催产素神经元记录的爆发中，振幅较小，发作时间较晚，而爆发跨度比在非乳胶侧记录的爆发时间短。这些结果表明，催产素神经元爆发的幅度和时间过程主要取决于其相反一侧的乳头的哺乳信息。（2）单侧哺乳对牛奶射精对催产蛋白神经元的牛奶的影响。幼崽仅限于与记录的催产量神经元相反的乳头，爆发幅度明显低于双侧哺乳期。此外，同侧哺乳期间的爆发幅度明显低于对侧哺乳期间的爆发幅度。发现表明，爆发幅度主要取决于由与重新定位一侧相对的nipplies产生的传入哺乳信号的量。在双侧和对侧哺乳过程中，广泛的半球间切片并没有废除催产素神经元的爆发，而是减少了在同侧哺乳过程中表现出的催产量神经元的数量。结果表明，下丘脑每一侧都有两个独立的门和求和机制。

项目成果

Wang,Y-F.,Negoro,H.,Honda,K.: "Effects of hemitransection of the medbrain on milk-ejection burst of oxytocin neurones in lactating rat." Journal of Endocrinology. 144. 463-470 (1995)

Wang，Y-F.，Negoro，H.，Honda，K.：“中脑半横切对哺乳大鼠催产素神经元乳汁喷射爆发的影响”。

Wang, Y-F., Negoro, H., Honda, K.: "Effects of hemitransection of the medbrain on milk-ejection burst of oxytocin neurones in lactating rat." J. Endocrinology. 144. 463-470 (1995)

Wang, Y-F.、Negoro, H.、Honda, K.：“中脑半横断对哺乳大鼠催产素神经元喷乳爆发的影响”。