Compilation of the vibosome-related protein seguences and its application to the analysis on eukavyotic cellular evalution

病毒体相关蛋白序列的编译及其在真核细胞评价分析中的应用

• 批准号: 07680742
• 负责人: HASHIMOTO Tetsuo
• 金额: $ 1.47万
• 依托单位: THE INSTITUTE OF STATISTICAL MATHEMATICS
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07680742/
• 关键词: ribosomal protein; molecular Evolution; alignment; molecular phylogenetic tree; eukaryotic cell; 下等真核生物

Large number of ribosomal protein (r-protein) sequences form various organisms have been accumulated up to now in the sequence databases. Although these data might have many informations on the evolution of the ribosomes, these are not well arranged in order because nomenclatures are various among different organisms, In this work we have systematically compiled the r-protein species, evolutionarily analyzing sequence similarities.On the basis of all the alignments constructed and phylogenetic analyzes carried out up to now, the evolutionary profile of r-proteins is emerging : (1) in no small number there are protein species that exist throughout the three primary kingdoms, (2) almost all the alignments show 'one to one' correlation to align regularly, that is irregular correlation such as internal transposition appears to be rarely found, (3) wide insertion/deletions and elongation in length are usually found in the comparisons among sequences from different primary kingdoms, while within a kingdom most of the evolutionary changes are amino acid replacements and minor insertion/deletions, (4) gene duplication events have occurred on several r-protein species in the various stages of eukaryotic evolution, (5) archaebactria do not show a monophyletic clade in no small number of r-protein species.On the other hand, we began to analyze the genes of ribosome-related molecules from amitochondriate protists, Glugea plecoglossi, Giardia lamblia and Trichomonas vaginalis to elucidate early evolution of eukaryotic ribosomes.

到目前为止，在序列数据库中已经积累了各种生物的核糖体蛋白（R蛋白）序列。尽管这些数据可能对核糖体的演变有很多信息，但由于命名的术语在不同生物体之间是各种各样的，这些数据并不是很好的排列，在这项工作中，我们系统地汇总了R蛋白质物种，从而在进化中分析了序列相似性。基于基础。在迄今为止进行的所有构造和系统发育分析中，R蛋白的进化特征正在出现：（1）在整个三个主要王国中存在蛋白质物种，（2）几乎所有对齐显示“一对一”与定期对齐的相关性，这是不规则的相关性，例如内部换位似乎很少被发现，（3）（3）在不同原发王国的序列之间的比较中，通常可以在比较中发现宽的插入/缺失和延长的长度，而长度则发现在一个王国中，大多数进化变化是氨基酸的替代品和少量插入/缺失，（4）在真核进化的各个阶段，在几种R蛋白质物种上发生了基因复制事件，（5）考古baccractria并未显示出单一的分支另一方面，我们开始分析来自阿米替恰会生物，葡萄菌，lamblia和trichomonas agninalis阴道的核糖体相关分子的基因，以阐明真核核糖体的早期进化。

项目成果

Yamamoto,A.,Hashimoto,T.,et al.: "Phylogenetic position of mitochondrion-lacking protozoan,Trichomonas tenax,based on amino acid sequences of elongation factors 1α and 2" Journal of Molecular Evolution. 44. 98-105 (1997)

Yamamoto, A., Hashimoto, T. 等人：“基于延伸因子 1α 和 2 的氨基酸序列，缺乏线粒体的原生动物 Trichomonas tenax 的系统发育位置”，分子进化杂志 44. 98-105 (1997)。 ）

Kamaishi,T.,Hashimoto,T.,Nakamura,Y.,Masuda,Y.,Nakamura,F.,Okamoto,K.,Shimizu,M.and Hasegawa,M.: "Complete nucleotide sequences of the genes encoding translation elongation factors 1α and 2 from a microsporidian parasite,Glugea plecoglossi: implications f

Kamaishi, T.、Hashimoto, T.、Nakamura, Y.、Masuda, Y.、Nakamura, F.、Okamoto, K.、Shimizu, M. 和 Hasekawa, M.：“编码翻译延伸的基因的完整核苷酸序列来自微孢子虫寄生虫 Glugea plecoglossi 的因子 1α 和 2：影响 f

Mizuta,K.,Hashimoto,T.and Otaka,E.: "The evolutionary relationships between homologs of ribosomal YL8 protein and YL8-like proteins." Current Genetics. 28. 19-25 (1995)

Mizuta,K.、Hashimoto,T. 和 Otaka,E.：“核糖体 YL8 蛋白和 YL8 样蛋白的同源物之间的进化关系。”

Mizuta,K.,Park,J.S.,et al.: "R1C1,a novel gene required for ribosome synthesis in Saccharomyces cerevisiae" Gene. (in press).

Mizuta,K.,Park,J.S.等人：“R1C1，酿酒酵母中核糖体合成所需的新基因”基因。

Kamaishi,T.,Hashimoto,T.,et al.: "Protein phylogeny of translation elongation factor EF-1α suggests Microsporidians are extremely ancient eukaryotes" Journal of Molecular Evolution. 42. 257-263 (1996)

Kamaishi, T.、Hashimoto, T. 等人：“翻译延伸因子 EF-1α 的蛋白质系统发育表明微孢子虫是极其古老的真核生物”《分子进化杂志》42. 257-263 (1996)。