CALCIFICATION MECHANISM OF THE OSTEOID IN THE PERIODONTAL LIGAMENT ON THE TENSION SIDE DURING TOOTH MOVEMENT.

牙齿移动过程中张力侧牙周韧带骨样的钙化机制。

基本信息

批准号：
07672245
负责人：
NAKAMURA Yoshiki
金额：
$ 1.47万
依托单位：
TSURUMI UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1996
项目状态：
已结题

项目摘要

Elemental analysis (EPMA) and detection of free Ca^<2+> were performed in the osteoid of the periodontal ligament (tension side) during the tooth movement in rats, using unfixed and undecalcified frozen sections. The uptake of Ca^<45> into the osteoid was examined by autoradiography.Upper first molars of mature male rats were moved seven days, and the upper jaws were fixed in liquid nitrogen. The unfixed and undecalcified frozen sections of the molar periodontal ligament were prepared for histological and histochemical (alkaline phosphatase activity : azo-dye method) observation and also for EPMA.In the detection of free-Ca, fluo-3, a fluorescent free Ca indicator, was injected from the hearts of the rats 60 sec.before freeze fixation by liquid nitrogen.In regard to the Autoradiography, Ca^<45> radioisotope was also injected intraperitoneally 60 min.before perfusion fixation of glutaraldehyde. Then the upper jaws were processed for Autoradiography.A bone surface in the buccal periodont … More al ligament (tension side) showed a wavy configuration, and a considerable amount of osteoid was formed. Intense ALP ase activity was noticed in the osteoid and osteoblastic layr. EPMA on the osteoid revealed extremely high peaks of P and Ca, that dropped a bruptly at first and then declined gradually, as the analyzed site moved away from the edge of the bone. S and K maintained steady peaks throughout the whole analyzed sites. Line analyzes demonstrated constant synchronization for the changes of P and Ca.Green fluorescence, showing the presence free Ca^<2+>, was emitted from the whole periodontal space and pulp cavity. The fluorescence from the osteoid was more intense than that from the periodontal ligament. EPMA data showed higher peak of Ca in the osteoid than in the periodontal ligament.Ca^<45> was deposited deeply in the osteoid of the buccal periodontal ligament (tension side) in 60 min.after the peritoneal injection.These results indicate that the osteoid concentration of P and Ca is elevated in the calcification and their concentration regulated precisely, and also that the Ca was supplied from the capillary, some of which is present in the form of ion in the osteoid. Less

在大鼠的牙齿运动过程中，使用未固定和未降低的冷冻切片在大鼠的牙齿移动过程中，在牙周韧带（张力侧）的骨质骨骼中进行了元素分析（EPMA）和游离Ca^<2+>的检测。通过自显影检查了Ca^<45>对骨质的摄取。将成熟雄性大鼠的第一颗磨牙移动了7天，上颌骨被固定在液氮中。制备了摩尔牙周韧带的未固定和未归化的冷冻切片，以进行组织学和组织化学（碱性磷酸酶活性：Azo-Dye方法）观察以及EPMA.在file-Ca，Fluo-3，Fluo-3，Fluo-3的检测，floororescent Freeca指标的检测中，由60秒的人注入了60秒的范围。放射自显影，Ca^<45>放射性同位素也被注入腹膜内60分钟。在灌注戊二醛之前。然后将上颌骨处理用于自显影。在骨质和成骨细胞层中发现了强烈的ALP ASE活性。骨质上的EPMA揭示了P和Ca的极高峰，随着分析位点从骨头边缘移开时，该P和Ca最初逐渐下降，然后逐渐下降。 K在整个分析位点保持稳定的峰值。线分析表明，P和Ca.Green荧光的变化的恒定同步，显示了自由Ca^<2+>的存在，是从整个牙周空间和纸浆腔中排出的。与牙周韧带的荧光相比，术的荧光更强烈。 EPMA数据显示，骨质中的Ca的峰值比牙周韧带更高。CA^<45>在60分钟内深入沉积在颊牙齿牙齿韧带（张力侧）的骨骨中。这些结果表明，在计算中，P和Ca的骨素浓度升高，其浓度精确地调节，并且Ca是从毛细管中提供的，其中一些以离子形式呈现在骨质骨中的离子形式。