CALCIFICATION MECHANISM OF THE OSTEOID IN THE PERIODONTAL LIGAMENT ON THE TENSION SIDE DURING TOOTH MOVEMENT.

牙齿移动过程中张力侧牙周韧带骨样的钙化机制。

基本信息

批准号：
07672245
负责人：
NAKAMURA Yoshiki
金额：
$ 1.47万
依托单位：
TSURUMI UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1996
项目状态：
已结题

项目摘要

Elemental analysis (EPMA) and detection of free Ca^<2+> were performed in the osteoid of the periodontal ligament (tension side) during the tooth movement in rats, using unfixed and undecalcified frozen sections. The uptake of Ca^<45> into the osteoid was examined by autoradiography.Upper first molars of mature male rats were moved seven days, and the upper jaws were fixed in liquid nitrogen. The unfixed and undecalcified frozen sections of the molar periodontal ligament were prepared for histological and histochemical (alkaline phosphatase activity : azo-dye method) observation and also for EPMA.In the detection of free-Ca, fluo-3, a fluorescent free Ca indicator, was injected from the hearts of the rats 60 sec.before freeze fixation by liquid nitrogen.In regard to the Autoradiography, Ca^<45> radioisotope was also injected intraperitoneally 60 min.before perfusion fixation of glutaraldehyde. Then the upper jaws were processed for Autoradiography.A bone surface in the buccal periodont … More al ligament (tension side) showed a wavy configuration, and a considerable amount of osteoid was formed. Intense ALP ase activity was noticed in the osteoid and osteoblastic layr. EPMA on the osteoid revealed extremely high peaks of P and Ca, that dropped a bruptly at first and then declined gradually, as the analyzed site moved away from the edge of the bone. S and K maintained steady peaks throughout the whole analyzed sites. Line analyzes demonstrated constant synchronization for the changes of P and Ca.Green fluorescence, showing the presence free Ca^<2+>, was emitted from the whole periodontal space and pulp cavity. The fluorescence from the osteoid was more intense than that from the periodontal ligament. EPMA data showed higher peak of Ca in the osteoid than in the periodontal ligament.Ca^<45> was deposited deeply in the osteoid of the buccal periodontal ligament (tension side) in 60 min.after the peritoneal injection.These results indicate that the osteoid concentration of P and Ca is elevated in the calcification and their concentration regulated precisely, and also that the Ca was supplied from the capillary, some of which is present in the form of ion in the osteoid. Less

采用未固定、未脱钙的冰冻切片，对大鼠牙齿移动过程中牙周膜（张力侧）的骨样体进行元素分析（EPMA）和游离Ca^2+的摄取。将成熟雄性大鼠的上颌第一磨牙移动7天，并将上颌固定在液氮中。磨牙牙周膜准备用于组织学和组织化学（碱性磷酸酶活性：偶氮染料法）观察以及 EPMA。在游离 Ca 的检测中，从磨牙的心脏注射荧光游离 Ca 指示剂 Fluo-3。大鼠液氮冷冻固定前60秒。关于放射自显影，还腹腔内注射Ca^45放射性同位素60分钟。在戊二醛灌注固定之前，对上颌进行放射自显影。颊侧牙周韧带（张力侧）的骨表面呈波状结构，并形成大量的类骨质，具有强烈的 ALP 酶活性。在类骨质和成骨细胞层中注意到，类骨上的 EPMA 显示出极高的 P 和 Ca 峰值，首先突然下降，然后逐渐下降，当分析位点远离骨边缘时，在整个分析位点中，S 和 K 稳定峰值显示出 P 和 Ca 变化的恒定同步。绿色荧光，表明存在游离 Ca^<2+ >。，从整个牙周间隙和牙髓腔发出的荧光比牙周韧带的荧光更强，显示骨样中的 Ca 峰值高于牙周组织中的峰值。腹腔注射后60分钟，Ca^<45>深深沉积在颊侧牙周膜（张力侧）的类骨质中。这表明钙化区中P和Ca的类骨质浓度升高，并且它们的浓度精确调节，并且 Ca 是由毛细血管供应的，其中一些以离子形式存在于骨样中。