THE EFFECT OF OVERPRODUCTION OF ENDOLYMPH ON THE INNER EAR FUNCTION

内淋巴分泌过多对内耳功能的影响

基本信息

批准号：
07671863
负责人：
TAKEDA Taizo
金额：
$ 1.47万
依托单位：
Kochi Medical School
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1997
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07671863/
关键词：
Overproduction of endolymph Endolymphatic hydrops Endocochlear potential Action potential of the cochlear nerve TEOA DPOAE K^+ activity 前庭神経自発活動電位人工内リンパ液 Negative EP DP-OAE

项目摘要

In order to study the effect of an overproduction of the endolymph on the inner ear function, artificial endolymph was injected into the scala media in animals with the experimentally-induced endolymphatic hydrops, and the auditory and vestibular functions were examined. The results were as follows : 1) Endolymphatic hydrops was induced by electro-cauterization of the endolymphatic sac. Moderate or severe hydrops developed 2 months after the cauterization. In these animals, the positive and negative endocochlear potentials had already decreased. Artificial endolymph injection into the cochlear duct produced no significant alteration in these potentials, although the negative endolymphatic potential decreased in normal animals. 2) The compound action potentials and cochlear microphonics also decreased in animals with experimentally induced hydrops. These potentials showed no change before and after the artificial endolymph injection. The delayd CM could not be detected in the present se … More ries of experiments. 3) Transient evoked otoacoustic emission (TEOA) was detected 2 weeks after the cauterization of the sac, but disappeared after 2 months. Thus, the effect of artificial endolymph injection to TEOAE was not investigated. Distortion product otoacoustic emission (DPOAE) could be recorded, but we could not examine the influence of artificial endolymph injection on DPOAE because of its instability. 4) Artificial endolymph injection into the cochlear duct produced no alteration in spontaneous discharge of the vestibular nerve either in normal animals or in animals with experimentally induced hydrops. 5) artificial endolymph injection into the cochlear duct produced no alteration in K^+ activity in the scala tympani either in normal animals or in animals with experimentally induced hydrops.In the previous studies, cochlear and vestibular dysfunction seemed to be attributed to high concentration of K ^+ in the scala media due to the leakage of endolymph via extended Reissner's membrane. However, the present results did not favor this hypothesis. Less

为了研究内淋巴过度产生对内耳功能的影响，将人工内淋巴注入实验性诱发内淋巴积液的动物的中阶，并检查其听觉和前庭功能，结果如下。：1)内淋巴囊电烧引起内淋巴积水，在这些动物中，烧灼后2个月出现中度或重度积水。尽管正常动物的负内淋巴电位降低了，但人工内淋巴注射到耳蜗管中并没有产生显着的变化。 2) 在实验诱导的水肿动物中，复合动作电位和耳蜗颤音也降低了。 . 在本系列实验中，无法检测到人工内淋巴注射前后的这些电位变化 3)。囊烧灼后 2 周检测到瞬时诱发耳声发射 (TEOA)，但 2 个月后消失。因此，未研究人工内淋巴注射对 TEOAE 的影响，但可以记录失真产物耳声发射 (DPOAE)。由于其不稳定性，我们无法检查人工内淋巴注射对 DPOAE 的影响。 4) 人工内淋巴注射入耳蜗管不会产生任何改变。在正常动物或实验性诱发水肿的动物中，前庭神经的自发放电5）人工内淋巴注射到耳蜗管中，无论在正常动物还是实验性诱发的动物中，鼓阶中的K^+活性均未发生改变。在之前的研究中，耳蜗和前庭功能障碍似乎是由于内淋巴通过扩展的赖斯纳氏漏泄而导致中阶中高浓度的 K^+ 所致。然而，目前的结果并不支持这一假设。