Prevalence of Human Papillomavirus in Cervical Cancer in Okinawa

冲绳人乳头瘤病毒在宫颈癌中的患病率

基本信息

批准号：
07671809
负责人：
HIGASHI Masahiro
金额：
$ 1.47万
依托单位：
University of the Ryukyus
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1997
项目状态：
已结题

项目摘要

1)Prevalence of human papillomavirus (HPV) in the screening smear test for cervical cancer in OkinawaHPV infection has now been shown to be the primary cause of most cases of cervical intraepithelial neoplasia and is a necessary, but not sufficient, cause of most cases of cervical cancer. HPV testing relies on the detection of viral deoxyribonucleic acid (DNA) in exfoliated cell specimens.We examined the correlation between the progression of cervical dysplasia and the prevalence of HPV by means of the polymerase chain reaction (PCR).The PCR with L1 consensus primers could identify at least nine HPV types, 6,11,16,18,31,33,42,52 and 58 (6), and the detailed typing of HPVs is needed in our study.Cervical scrapes were collected from 3,985 women who received the screening smear test for cervical cancer in Okinawa.The prevalence of HPVs in normal cervices (Pap class I and II) was that under 30 years old : 18.7% (45/241), 31-40 years old : 8.0% (47/587), 41-50yrs : 9.3% (76/817), 51-60yrs : … More 10.0% (106/1059), 61-70yrs : 10.2% (104/1018) and over 70yrs : 9.8% (26/263).At present, we are investigating, prospectively, the prevalence of HPV DNA after treatment and the predictive value of HPV positivity for recurrence of abnormal cytology and cervical intraepithelial neoplasias (CIN). Our study indicates that detectability of HPV DNA does coincide with detectability of CIN and that effective surgical treatment of CIN can also eliminated detectable presence of HPV DNA.2)Clinical significance of human papillomavirus detection from metastasis-suspected tissues in cervical cancer.Studies on Human papillomaviruses (HPVs) are prevalent in primary diseases of cervical cancer. The vast majority of these studies have been done using the technique to detect HPV DNAs in paraffin-embedded tissues. On the other hand, only limited data are available on the detection of HPV DNAs in metastatic lesions.The aim of the present study was to establish the procedure of DNA extraction from fresh tissue specimens for HPV analysis and to examine the correlation between the presence of HPVs and pathological evidence in metastasis-suspected tissues.CONCLUSION1.HPV DNAs were detectable in cell samples prepared from fresh metastasis-suspected tissues as well as primary cervical lesions.2.HPV type in metastatic lesions was identical with that in their primary cervical lesions in all of HPV-positive patients.3.Of 6 cases with HPV-positive nodes not histologically proven to be metastasized, 3 suffered from recurrence a few months later at the sites where the HPV-positive nodes were located, suggesting that the nodes had been already metastasized at the time of sampling.4.HPV-positive detection in metastasis-suspected tissues could provide early information for the appraisal of possible metastasis. Less

1）现在已证明人乳头瘤病毒（HPV）在筛查宫颈癌的筛查测试中，现已证明是大多数宫颈上皮内肿瘤病例的主要原因，是必要的，但不足以但不足，但大多数宫颈癌病例。 HPV测试依赖于在剥落细胞标本中检测病毒脱氧核糖核酸（DNA）。我们检查了宫颈发育不良的进展与通过聚合酶链反应（PCR）的颈椎发育不良的进展与HPV的患病率之间的相关性。 6,11,16,18,31,31,33,42,52和58（6），以及我们的研究中需要详细的HPV键入。从3,985名妇女那里收集了颈椎刮擦，这些妇女是从Okinawa接受宫颈癌的筛查筛查测试的3,985名女性。（45/241），31-40岁：8.0％（47/587），41-50yr：9.3％（76/817），51-60yrs：…更多10.0％（106/1059），61-70yrs，61-70yrs：10.2％（104/1018）（104/1018）和70yrs and 70yrs and 70yrs：9.8％（26/9.88％）目前，我们正在研究治疗后HPV DNA的患病率以及HPV潜力异常细胞学和宫颈上皮内肿瘤（CIN）复发的预测价值。我们的研究表明，HPV DNA的可检测性确实与CIN的可检测性相吻合，并且CIN的有效手术治疗也可以消除可检测的HPV DNA的可检测性。2）人乳头瘤病毒检测到宫颈癌中的人乳头瘤病毒检测的临床意义。对人乳头瘤病毒的研究。这些研究中的绝大多数是使用该技术来检测石蜡包含组织中的HPV DNA的。另一方面，仅在转移性病变中检测HPV DNA的数据有限。本研究的目的是建立从新鲜组织样品中提取DNA的程序，以进行HPV分析，并检查HPV的存在与在转移症中的病理证据之间的相关性。 2. HPV类型的转移性病变类型与所有HPV阳性患者的主要宫颈病变相同。3。6例HPV阳性淋巴结的6例未在组织学上被证明是在hpv-ways sites中遭受的，该疾病已经在hpv-posv pers上恢复了。在采样时进行转移。4.HPV阳性的组织中的HPV阳性检测可以提供早期信息，以评估可能的转移。较少的