Prevalence of Human Papillomavirus in Cervical Cancer in Okinawa

冲绳人乳头瘤病毒在宫颈癌中的患病率

基本信息

批准号：
07671809
负责人：
HIGASHI Masahiro
金额：
$ 1.47万
依托单位：
University of the Ryukyus
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1997
项目状态：
已结题

项目摘要

1)Prevalence of human papillomavirus (HPV) in the screening smear test for cervical cancer in OkinawaHPV infection has now been shown to be the primary cause of most cases of cervical intraepithelial neoplasia and is a necessary, but not sufficient, cause of most cases of cervical cancer. HPV testing relies on the detection of viral deoxyribonucleic acid (DNA) in exfoliated cell specimens.We examined the correlation between the progression of cervical dysplasia and the prevalence of HPV by means of the polymerase chain reaction (PCR).The PCR with L1 consensus primers could identify at least nine HPV types, 6,11,16,18,31,33,42,52 and 58 (6), and the detailed typing of HPVs is needed in our study.Cervical scrapes were collected from 3,985 women who received the screening smear test for cervical cancer in Okinawa.The prevalence of HPVs in normal cervices (Pap class I and II) was that under 30 years old : 18.7% (45/241), 31-40 years old : 8.0% (47/587), 41-50yrs : 9.3% (76/817), 51-60yrs : … More 10.0% (106/1059), 61-70yrs : 10.2% (104/1018) and over 70yrs : 9.8% (26/263).At present, we are investigating, prospectively, the prevalence of HPV DNA after treatment and the predictive value of HPV positivity for recurrence of abnormal cytology and cervical intraepithelial neoplasias (CIN). Our study indicates that detectability of HPV DNA does coincide with detectability of CIN and that effective surgical treatment of CIN can also eliminated detectable presence of HPV DNA.2)Clinical significance of human papillomavirus detection from metastasis-suspected tissues in cervical cancer.Studies on Human papillomaviruses (HPVs) are prevalent in primary diseases of cervical cancer. The vast majority of these studies have been done using the technique to detect HPV DNAs in paraffin-embedded tissues. On the other hand, only limited data are available on the detection of HPV DNAs in metastatic lesions.The aim of the present study was to establish the procedure of DNA extraction from fresh tissue specimens for HPV analysis and to examine the correlation between the presence of HPVs and pathological evidence in metastasis-suspected tissues.CONCLUSION1.HPV DNAs were detectable in cell samples prepared from fresh metastasis-suspected tissues as well as primary cervical lesions.2.HPV type in metastatic lesions was identical with that in their primary cervical lesions in all of HPV-positive patients.3.Of 6 cases with HPV-positive nodes not histologically proven to be metastasized, 3 suffered from recurrence a few months later at the sites where the HPV-positive nodes were located, suggesting that the nodes had been already metastasized at the time of sampling.4.HPV-positive detection in metastasis-suspected tissues could provide early information for the appraisal of possible metastasis. Less

1）冲绳宫颈癌筛查涂片检查中人乳头瘤病毒（HPV）的患病率现已证明HPV感染是大多数宫颈上皮内瘤变病例的主要原因，并且是大多数宫颈上皮内瘤变病例的必要但非充分原因宫颈癌的检测依赖于脱落细胞标本中病毒脱氧核糖核酸 (DNA) 的检测。我们研究了宫颈发育不良的进展与患病率之间的相关性。通过聚合酶链式反应 (PCR) 检测 HPV。使用 L1 共有引物的 PCR 可以鉴定至少 9 种 HPV 类型：6、11、16、18、31、33、42、52 和 58 (6)，详细信息我们的研究需要对 HPV 进行分型。从冲绳岛接受宫颈涂片筛查的 3,985 名女性中收集了宫颈刮片。正常人中 HPV 的患病率子宫颈抹片检查（巴氏涂片 I 级和 II 级）的比例为 30 岁以下：18.7% (45/241)、31-40 岁：8.0% (47/587)、41-50 岁：9.3% (76/817)、51 -60 岁：……更多 10.0% (106/1059)，61-70 岁：10.2% (104/1018) 70 岁以上：9.8% (26/263)。目前，我们正在前瞻性地研究治疗后 HPV DNA 的患病率以及 HPV 阳性对异常细胞学和宫颈癌复发的预测价值。我们的研究表明，HPV DNA 的可检测性与 CIN 的可检测性一致，并且 CIN 的有效手术治疗也可以消除可检测到的病毒。 HPV DNA的存在。2）从宫颈癌疑似转移组织中检测人乳头瘤病毒的临床意义。关于人乳头瘤病毒（HPV）的研究在宫颈癌的原发性疾病中普遍存在，其中绝大多数研究都是使用该技术完成的。检测石蜡包埋组织中的 HPV DNA 另一方面，关于转移性病变中 HPV DNA 检测的数据有限。本研究的目的是建立该程序。从新鲜组织标本中提取 DNA 进行 HPV 分析，并检查 HPV 的存在与疑似转移组织中的病理证据之间的相关性。结论 1. 在新鲜转移疑似组织和原发宫颈组织制备的细胞样本中可检测到 HPV DNA。 2. 所有 HPV 阳性患者中，转移性病变的 HPV 类型与其原发性宫颈病变的 HPV 类型相同。 3. 6 例 HPV 阳性淋巴结未经组织学证实其中 3 例在几个月后在 HPV 阳性淋巴结所在部位复发，提示采样时淋巴结已发生转移。 4.疑似转移组织中 HPV 阳性检测可以提供用于评估可能转移的早期信息较少。