New Therapeutic omega-3 unsaturated fatty acid for nonspecific inflammatory colonic diseases.

用于非特异性炎症性结肠疾病的新型治疗性 omega-3 不饱和脂肪酸。

• 批准号: 07807125
• 负责人: NAKASAKI Hisao
• 金额: $ 1.15万
• 依托单位: Tokai University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07807125/
• 关键词: non specific inflammatory colonic disease; TNBS-induced colitis; omega-3 type fatty acid; fish oil

The causes of nonspecific inflammatory diseases of the intestinal tract--Crohn's disease, ulcerative colitis, and intestinal Behcet's disease--are unknown. The remedies for these afflictions are total parenteral nutrition and the administration of steroids and salazosulfa pyridine immunosppressants. It is known, however, that total parenteral nutrition over a long period leads to a decline in local immunocompetence, accompanied by disused atrophy of the intestinal tract. This produces expansion of an auxiliary lesion of the inflammatory diseases and delayd healing.The purposes of the research were as follow ;(1) (a) Preparatory development targeting the creation of an absorbable EPA,which has a superior antiinflammatory effects. (b) The establishment of preparatory technology for emulsification by the use of lecithin, and (c) the creation of an emulsion that includes omega-3 fat by mixing soybean oil and fish oil. (2) Examination of the effectiveness of the pervenous-administration met … More hod, by using the newly developed fat nutrient on diseased-animals' models. (3) Confirmation of the effect as a fat nutrient (a) by administering an appropriate mixture of fish oil to animal models, (b) by analyzing the fatty acid in the serum, and (c) by examining the histological findings and the state of the biological function of the intestinal mucous membranes.The results ;TPN solutions with various fatty acid compositions were administered. Soybean contains 6.9% omega-3 fatty acid and fish oil containns 32.1% of omega-3 fatty acid. The fat emulsions and their relative proportions used in the respective group were as follows :Group I : Soybean oil emulsion only.Group II : mixture of 75% soybean oil and 25% fish oil emulsion.Group III : mixture of 50% soybean oil and 50% fish oil emulsion.Group IV : mixture of 25% soybean oil and 75% fish oil emulsion.Group V : fish oil emulsion only.Group VI : no fat emulsion administered.After completion of the 7-day TPN period the rats were laparotomized under ether anesthesia. The degree of healing of ulcerative colitis was assessed by HE staining of the colitis region and grading in accordance with the criteria.The severity of colitis was least in Group II (containing 13.2% of omega-3 fatty acid), and was relatively greater in Group III,I,IV and VI in that order.Group II displayd a significantly lower degree of severity as compared with group VI according to the Dunnett multiple comparison method and also as compared with group V according to the Tukey multiple comparison method. The result indicates that administration of omega-3 essential fatty acids has an anti-inflammatory effect. Moreover, the results indicated that healing effects could not be expected from administration of fat emulsions containing only soybean oil (Group I).Further studies are necessary in order to ascertain the concentrations between appropriate fish oil dosages. Less

肠道疾病，溃疡性结肠炎和肠道behcet病的非特异性炎症性疾病的原因是未知的。这些感情的补救措施是总父母营养和类固醇和萨拉唑磺胺吡啶免疫抑制剂的给药。然而，众所周知，长期以来，父母的总营养会导致局部免疫能力下降，这是由于肠道萎缩而实现的。这会增加炎症性疾病的辅助病变和延迟的愈合。研究的目的如下；（1）（a）针对可吸收可吸收的EPA的预备发展，其具有超级抗炎作用。 （b）通过使用卵磷脂建立用于乳化的制备技术，以及（c）创建包括欧米茄3脂肪的乳化技术，通过混合大豆油和鱼油。 （2）通过使用新开发的脂肪营养素在解散的动物模型上使用新开发的脂肪营养素来遇到更多的HOD。 （3）通过对血清中的脂肪酸进行适当的混合物和（c）检查脂肪酸，通过检查肠道粘膜的生物学功能来确认其作为脂肪营养物质（a），（a）通过分析血清中的脂肪酸和（c）来确认作用。大豆含有6.9％的omega-3脂肪酸，鱼油含有32.1％的omega-3脂肪酸。相对组中使用的脂肪乳液及其相对比例如下：I组：仅大豆油乳液。ii组：75％豆油和25％鱼油乳液的混合物III：50％大豆油和50％鱼油乳液的混合物IV：25％的FISHBER OIL。唯一的。群VI：不给予脂肪乳液。在7天TPN期间完成后，大鼠在乙醚麻醉下进行了laparotomom。通过染色结肠区域的染色并根据标准评估溃疡性结肠炎的愈合程度。结肠炎的严重程度在II组中最少（含有13.2％的omega-3脂肪酸），并且在III，III，IV和VI中相对较大，根据II的多种程度和VI的比较，与该方法相比，VI的程度非常低。与第V组相比，根据Tukey多重比较方法。结果表明，omega-3必需脂肪酸的给药具有抗炎作用。此外，结果表明，只能通过含有大豆油的脂肪乳液（I组）进行治疗。为了确定适当的鱼油剂量之间的浓度是必要的。较少的