Study on the model for the vulnerability to stress using stress-induced c-fos mRNA expression as an index of stress response.

以应激诱导的c-fos mRNA表达作为应激反应指标的应激脆弱性模型研究。

• 批准号: 07807092
• 负责人: WATANABE Yoshifumi
• 金额: $ 1.34万
• 依托单位: SAITAMA MEDICAL SHCOOL
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07807092/
• 关键词: vulnerability to stress; c-fos mRNA; in situ hybridization; Wistar Kyoto rats; paraventricle nucleus; locus coeruleus; Corticosterone; c-fos; In Situハイブリダイゼーション; 血中コルチコステロン濃度; ストレス; 脆弱性; モデル動物; in situ ハイブリダイゼーション

1.Strain comparison in c-fos mRNA expression induced by acute and repeated restraint stress.Wistar Kyoto (WKY) rats and Wistar rats were stressed by 1h restraint stress daily for one, three, seven and fourteen days. In these rats, stress-induced c-fos mRNA expression in paraventricle nucleus (PVN) and locus coeruleus (LC) were measured by in situ hybridization assay.To an acute restraint stress, WKY rats and Wistar rats showed similar c-fos mRNA expression in both PVN and LC.As reported before, Wistar rats showed substantial decrease in stress-induced c-fos mRNA expression in both PVN and LC on third stress day, and thereafter stress-induced c-fos mRNA expression decreased gradually. WKY rats, also, showed similar pattern of decrease in stress-induced c-fos mRNA expression in both PVN and LC by repeated restraint stress. Administration of imipramine, an antidepressant, for two weeks with repeated 1h restraint stress did not affect the decrement of stress-induced c-fos mRNA expression i … More n both PVN and LC in WKY rats.These results suggest that WKY rats are not sensitive and vulnerable to stress.2.Strain comparison in stress response of HPA axis and stress-induced anxiety.Plasma corticosterone (CS) were measured during 2h restraint stress on first, third, sixth and thirteenth stress day in WKY rats and Wistar rats.To an acute restraint stres, WKY rats showed higher peak CS level compared to Wistar rats. Wistar rats showed decrease in peak CS levels and other CS levels at 30min, 60min and 120min after the beginning of the stress from third stress day, and thereafter the decrement of CS levels were getting greater by thirteenth stress day, whereas WKY rats did not show the decrease in peak CS level by thirteenth stress day, and only CS levels at 120min after the beginning of the stress decreased from third stress day.To measure the stress-induced anxiety, we measures the freezing time after fifteen minits footshock stress. Wistar rats showed reduction of the freezing time from fifth stress day, whereas WKY rats did not show any reduction of the freezing time by thirteenth stress day.These results suggest the sensitivity and the vulnerability of WKY rats to stress. Less

1.急性和重复束缚应激诱导的c-fos mRNA表达的应变比较。Wistar京都(WKY)大鼠和Wistar大鼠每天受到1小时的束缚应激，持续1、3、7和14天。原位杂交法测定室旁核（PVN）和蓝斑（LC）诱导的c-fos mRNA表达。对于急性束缚应激，WKY大鼠和Wistar大鼠表现出相似的表现。 PVN 和 LC 中的 c-fos mRNA 表达。如前所述，Wistar 大鼠在应激第三天表现出应激诱导的 PVN 和 LC 中 c-fos mRNA 表达显着下降，此后应激诱导的 c-fos mRNA 表达下降通过反复给予丙咪嗪（一种抗抑郁药）两周并重复 1 小时，WKY 大鼠也逐渐表现出应激诱导的 PVN 和 LC 中 c-fos mRNA 表达的减少。 WKY大鼠的PVN和LC中束缚应激均不影响应激诱导的c-fos mRNA表达的减少。这些结果表明WKY大鼠对应激不敏感且易受影响。2.应激反应的应变比较HPA 轴和应激诱发的焦虑。在 WKY 大鼠和 Wistar 大鼠的第一、第三、第六和第十三个应激日的 2 小时约束应激过程中测量血浆皮质酮 (CS)。对于急性约束应激，WKY与Wistar大鼠相比，大鼠的CS峰值水平在应激第三天开始后30分钟、60分钟和120分钟出现峰值CS水平和其他CS水平下降，此后CS水平的下降幅度更大。到第13个应激日时，WKY大鼠没有表现出峰值CS水平在第13个应激日时下降，仅在应激开始后120分钟时CS水平从第3个应激日开始下降。我们测量了 15 分钟足部电击应激后的僵直时间，Wistar 大鼠从第 5 个应激日开始表现出僵直时间减少，而 WKY 大鼠到第 13 个应激日没有表现出任何僵直时间减少。这些结果表明了敏感性和稳定性。 WKY 大鼠对压力的脆弱性较低。