Evaluation of indterinidividual variation in susceptibility to mutagens

评估诱变剂敏感性的个体差异

• 批准号: 07670452
• 负责人: ODAGIRI Youichi
• 金额: $ 1.15万
• 依托单位: Yamanashi College of Nursing
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07670452/
• 关键词: mutagen; micronucleus; X-ray; colchicine; interindividual variance

In order to evaluate the individual susceptibility to mutagen, we have measured spontaneous or mutagen(X-ray or colchicine)-induced micronucleus frequency in human lymphocytes from a normal healthy population, and have analyzed statistically for interindividual variance. As a result, there was a significant difference in the mutagen-induced micronucleus frequency between individuals and this was indicative of interindividual variance in susceptibility to these mutagens. Then, we have investigated the relationship between these micronucleus levels and serum antioxidative activity or antioxidant micronutrients(vitaminsB12, CandE,beta-carotene and folic acid) levels. Serum antioxidative activity measured by the electron spin resonance spectrometry (ESR) was strongly correlated with the serum levels of water-soluble micronutrients such as Vitamin C and folic acid. Kinetochore-labelling study revealed that folic acid and vitamin C are major variables for induction of micronuclei in lymphocytes. Serum folic acid level was negatively correlated with the kinetochore-positive and-negative micronucleus frequency and vitamin C was positively related to the formation of kinetochore-positive micronuclei. These reults suggest that interindividual variance in chromosomal response to mutagens(X-ray and colchicine) in normal population may be a real phenomenon, and that individual susceptibilities to these mutagens are unrelated. Our data also suggest that the baseline micronucleus level which is strongly associated with the levels of certain serum micronutrients(folic acid and vitamin C) is not of predictive value for the susceptibilities to these mutagens.

为了评估诱变剂的个体易感性，我们测量了来自正常健康人群的人淋巴细胞中自发性或诱变剂（X射线或秋水仙碱）诱导的微核频率，并在统计学上分析了个体间差异。结果，诱变诱导的个体之间的微核频率存在显着差异，这表明对这些诱变剂的敏感性的个体差异。然后，我们研究了这些微核水平与血清​​抗氧化活性或抗氧化剂微量营养素（Vitaminsb12，Cande，β-胡萝卜素和叶酸）水平之间的关系。通过电子自旋谐振光谱法（ESR）测量的血清抗氧化活性与水溶性微量营养素（如维生素C和叶酸）的血清水平密切相关。动力学标志性研究表明，叶酸和维生素C是诱导淋巴细胞中微核的主要变量。血清叶酸水平与动力学阳性和阴性的微核频率负相关，维生素C与动型阳性微核的形成呈正相关。这些案例表明，正常人群中对诱变剂（X射线和秋水仙碱）染色体反应的个体差异可能是一个真正的现象，并且对这些诱变剂的个别敏感性是无关的。我们的数据还表明，与某些血清微量营养素（叶酸和维生素C）的水平密切相关的基线微核水平并不具有对这些诱变剂的敏感性的预测价值。

项目成果

Y.Odagiri and H.Uchida: "Relationship between spontaneous formation of micronuclei in human peripheral blood lympho-cytes and serum levels of antioxidant miaronutrients" (submitted).

Y.Odagiri 和 H.Uchida：“人外周血淋巴细胞中微核自发形成与抗氧化微量营养素血清水平之间的关系”（已提交）。

内田博之 ら: "ヒト血清中の抗酸化性ビタミン濃度とヒドロキシルラジカル消去活性の関係について" 山梨県立看護短期大学紀要. 3. 印刷中 (1998)

Hiroyuki Uchida 等：“人血清中抗氧化维生素浓度与羟自由基清除活性之间的关系”山梨县护理学院通报 3。出版中（1998 年）。

Y.Odagiri, H.Uchida and S.Sibazaki: "Interindividual variation in cytogenetic response to X-ray and colchicine measured with the cytokinesis-block micronucleus assay" Mutation Research. 381. 1-13 (1997)

Y.Odagiri、H.Uchida 和 S.Sibazaki：“用胞质分裂阻断微核测定法测量 X 射线和秋水仙碱的细胞遗传学反应的个体差异”突变研究。

Odagiri,Y.et al.: "Interindividual variation in cytogenetic response to X-ray and colchicine measured with the cytokinesis-block micronucleus assey" Mutation Research. 381. 1-13 (1997)

Odagiri,Y.et al.：“用胞质分裂阻断微核测定法测量细胞遗传学对 X 射线和秋水仙碱反应的个体差异”突变研究。

Odagiri, Y. et al.: "Interindividual variation in cytogenetic rosponse to X-ray and colchicine measured with the cytokinesis-block micronucleus assay." Mutation Research. 381. 1-13 (1997)

Odagiri, Y. 等人：“用胞质分裂阻断微核测定法测量细胞遗传学对 X 射线和秋水仙碱反应的个体差异。”