Immunological and molecular biological study in occupational, environmental and community health

职业、环境和社区健康领域的免疫学和分子生物学研究

基本信息

批准号：
07670424
负责人：
TANIGAWA Takeshi
金额：
$ 1.6万
依托单位：
University of Tsukuba
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1997
项目状态：
已结题

项目摘要

In this study, we measured minute lymphocyte subpopulations using two-color staining in subjects relevant to the fields of occupational and environmental medicine and community medicine. In addition, we tried to apply the molecular biology for solving the problems in occupational medicine. We obtained the following findings.1.We found a selective decrease in CD4+CD45RA+T cells in workers who had been exposed to aromatic amines. On the other hand, we found an increase in CD57+CD16 natural killer (NK) cells in these workers.2.A selective increase in CD4+CD29+T cells was found in smokers.3.By the analyzes controlling for the effects of smoking, we found (1) an increase in CD4+T cells in smokers and (2) the decreasing effects of chromates on CD4+T and CD8+T cells were stronger than those of aromatic amines.4.Both CD4+T and CD8+T cells were decreased in workers who had been exposed to chromates. Moreover, decreases in CD4+CD45RA+T cells and CD57+CD16+NK cells (with intermediate NK activity) were found in chromate workers.5.A significant decrease in CD3+CD45RO+(memory) T cells were found in lead workers after adjusting for the effects of age and smoking. We found the elevated IgG in 2 workers exposed to high level of lead after 10 (6) week injections of Ca-EDTA,suggesting the improved functions of antibody production.6.A significant decrease in CD4+CD45RA+T cells was found in Korean workers exposed to high level of mercury.7.We found no mutations in K-ras, H-ras and p53 genes in extracted DNA from samples of the primary tumors of malignant mesothelioma in 7 asbestos patients by the SSCP and sequencing methods.

在这项研究中，我们使用双色染色测量了职业和环境医学以及社区医学领域相关受试者的微小淋巴细胞亚群。此外，我们尝试应用分子生物学来解决职业医学中的问题。我们得到以下发现：1.我们发现接触过芳香胺的工人CD4+CD45RA+T细胞选择性减少。另一方面，我们发现这些工人的 CD57+CD16 自然杀伤 (NK) 细胞有所增加。2.在吸烟者中发现 CD4+CD29+T 细胞选择性增加。3.通过控制以下影响的分析吸烟后，我们发现（1）吸烟者的 CD4+T 细胞增加，（2）铬酸盐对 CD4+T 和 CD8+T 细胞的减少作用强于芳香胺。4.CD4+T 和 CD4+T 细胞均增加。接触过铬酸盐的工人的 CD8+T 细胞减少。此外，铬酸盐工人中CD4+CD45RA+T细胞和CD57+CD16+NK细胞（具有中等NK活性）减少。 5.调整后，铅工人CD3+CD45RO+（记忆）T细胞显着减少。年龄和吸烟的影响。我们发现 2 名接触高浓度铅的工人在注射 Ca-EDTA 10 (6) 周后 IgG 升高，表明抗体产生功能得到改善。 6. 韩国人发现 CD4+CD45RA+T 细胞显着减少7.SSCP从7例石棉患者的恶性间皮瘤原发肿瘤样本中提取的DNA中，未发现K-ras、H-ras和p53基因突变和测序方法。