Development of Evanescent Wave Light Scattering Microscope Instrument and Evaluation of Polymer Particle Dynamics and Interaction near Interface

倏逝波光散射显微镜仪器的研制及聚合物颗粒动力学和界面附近相互作用的评价

基本信息

批准号：
07555300
负责人：
YAMAOKA Hitoshi
金额：
$ 10.5万
依托单位：
KYOTO UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (A)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1997
项目状态：
已结题

项目摘要

The construction of the evanescent wave light scattering microscope (EVLSM) proved successful by the use of a novel technique combining the evanescent wave and the dynamic light scattering techniques. The dynamic and static characteristics of a polystyrene latex particle in dispersion interacting with a glass surface were studied by this technique. The dynamic behavior of the thermal vibration of the particle in a potential well created by electrostatic interaction between the particle and glass and gravity was clearly and quantitatively estimated, in addition to the estimation of the potential profile itself. The potential minimum became shallower with increasing added salt concentration, It was also clearly observed that the vibrational motion of the particle in the well became large in amplitude and the probability of the occurrence of the large vibration became large with increasing salt concentration. The potential profile of interaction between a charged liposome particle and glass surface was futhermore estimated directly by EVLSM, which can measure the distance between the particle and surface as a function of time at intervals in the order of less than ins, This new EVLSM technique was as well applied to the system between polystyrene latex particle and chemically-modified glass surface. Such information on the dynamics is essential for the correct understanding of the interaction potential. The EVLSM method is shown to be a very powerful technique for the estimation of not only the potential profilebut also dynamic characteristics.

通过使用新型技术结合了逃生的波浪和动态光散射技术，证明了逃生的波散射显微镜（EVLSM）的构造被证明是成功的。通过该技术研究了与玻璃表面相互作用的聚苯乙烯乳胶粒子的动态和静态特性。除了估计电位曲线本身外，粒子与玻璃与重力之间的静电相互作用以及玻璃与重力之间的静电相互作用产生的电势孔的热振动的动态行为也清楚地估计了。随着添加盐浓度的增加，潜在的最小值变得较浅，还清楚地观察到，井中粒子的振动运动在幅度上变大，并且随着盐浓度的增加，大振动的发生概率变得很大。电荷脂质体颗粒和玻璃表面之间相互作用的潜在曲线是由EVLSM直接估算的，EVLSM可以在粒子和表面之间的距离以小于INS的时间间隔来测量粒子和表面之间的距离，这种新的EVLSM技术是这种新的EVLSM技术。同时也应用于聚苯乙烯乳胶颗粒和化学改性玻璃表面之间的系统。有关动态的此类信息对于正确理解相互作用潜力至关重要。 EVLSM方法被证明是一种非常强大的技术，不仅可以估计潜在的概况BUT，还可以动态特征。