Study on transcription factor NF-kB regulating the gene function of tyrosin kinase in osteoblast

转录因子NF-kB调控成骨细胞酪氨酸激酶基因功能的研究

• 批准号: 07457436
• 负责人: MATSUMOTO Akitra
• 金额: $ 4.93万
• 依托单位: Hokkaido University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07457436/
• 关键词: Transcription factor; NF-kB; Osteoblastic MC3T3-E1 cells; Tyrosine kinase; Low-calcium environment; Gel retardation assay

We examined about the interaction between gene transcription factors and intracellular signal transduction, using the osteoblast-rich bone cells and osteoblastic Mc3T3-E1 cells(E1 cells)cultured under a low-calcium environment for the prevention of alveolarbone collapse seen in the patients with periodontal disease.In the bone cells cultured in a low-calcium medium, intracellular calcium content and free calcium concetration significantly decreased, compared with those in normal one (p<0.01)It was found that the binding sites of NF-kB,CREB and Oct-1 of gene transcription factors existed in the sequence in nuclear DNA in E1 cells. DNA-protein binding activity of NF-kB and Oct-1 seemed to be lower in the cells cultured in a low-calcium medium than in normal one. As there is electrophoretically the differencein the position of band of DNa-protein complex between E1 cells in noumal environment and that in a low-calcium one in CREB,it was shown that DNA-protein complex formation seemed to be disturved in a low-calcium environment in CREB.From these facts, the inhibitory interaction between the function of gene transcription factors and that of intracellular signal transduction seemed to be closely related with the alveolar bone collapse phenomenon in the patient with peiodontal disease.

我们研究了基因转录因子与细胞内信号转导之间的相互作用，使用成骨细胞富含成骨细胞的骨细胞和成骨细胞MC3T3-E1细胞（E1细胞）在低钙符环境下培养的，以预防牙周牙周神经乳杆菌塌陷患者的牙周肿瘤患者。疾病。在低钙培养基中培养的骨细胞，细胞内钙含量和游离钙伴随量显着降低，与正常钙（p <0.01）相比，发现NF-KB，CREB和OCT的结合位点基因转录因子的-1在E1细胞中的核DNA中存在。在低钙培养基中培养的细胞中NF-KB和OCT-1的DNA蛋白结合活性似乎低于正常培养基。由于在电泳上存在差异，在非环境中E1细胞之间的DNA-蛋白质复合物的位置，并且在CREB中的低钙含量中，DNA-蛋白质复合物的形成似乎在低钙中被扰乱从这些事实中，基因转录因子功能与细胞内信号转导的功能之间的抑制作用似乎与PE碘氏病患者的肺泡骨塌陷现象密切相关。

项目成果

Kobayashi K., Suzuki K., Takiya S., Yoshimura Y., Imai T., Matsumoto A., Nakamura S.: "Developmental profiles of phosphorylated and unphosphorylated CREBs in murine calvarial MC3T3-E1 cells" Journal of Biochemistry. In press.

Kobayashi K.、Suzuki K.、Takiya S.、Yoshimura Y.、Imai T.、Matsumoto A.、Nakamura S.：“小鼠颅骨 MC3T3-E1 细胞中磷酸化和非磷酸化 CREB ​​的发育概况”生物化学杂志。

Yoshimura Y., Hisada Y., Suzuki K., Deyama Y.and Matsumoto A.: "Effect of a low-calcium environment onalkaline phosphatase activity in embryonic ratcalvarial bone cells in culture." Archives of oral Biology. 41. 41-45 (1996)

Yoshimura Y.、Hisada Y.、Suzuki K.、Deyama Y. 和 Matsumoto A.：“低钙环境对培养的胚胎大鼠颅骨骨细胞碱性磷酸酶活性的影响。”

Deyama Y., Matsumoto A., Yoshimura Y.and Suzuki K.: "The effect of calcium ion on the c-fos mRNA expression in the clonal osteoblastic MC3T3-E1 cells derived from mouse calvaria." Oral Therapeutics and Pharmacology. 14. 132-137 (1995)

Deyama Y.、Matsumoto A.、Yoshimura Y. 和 Suzuki K.：“钙离子对源自小鼠颅骨的克隆性成骨细胞 MC3T3-E1 细胞中 c-fos mRNA 表达的影响。”

Deyama Y., Matsumoto A., Yoshimura Y., Suzuki K.and Hisada Y.: "The relation between c-fos mRNA expression and protein Kinase C activity in the MC3T3-E1 cells under a low calcium environment as a periodontal disease model." Oral Therapeutics and Pharmacol

Deyama Y.、Matsumoto A.、Yoshimura Y.、Suzuki K.和 Hisada Y.：“作为牙周病模型的低钙环境下 MC3T3-E1 细胞中 c-fos mRNA 表达与蛋白激酶 C 活性的关系

Deyama Y., Matsumoto A., Yoshimura Y.andSuzuki K.: "The effect of calcium ion on the c-fosmRNA expression in the clonal osteoblastic MC3T3-E1 cells derived from mouse calvaria." Oral Therapeutics and Pharmacology. 14. 132-137 (1995)

Deyama Y.、Matsumoto A.、Yoshimura Y.和Suzuki K.：“钙离子对小鼠颅骨来源的克隆性成骨细胞 MC3T3-E1 细胞中 c-fosmRNA 表达的影响。”