Mechanism of descending pain control system in the spinal cord

脊髓下行疼痛控制系统的机制

• 批准号: 07457351
• 负责人: BABA Hiroshi
• 金额: $ 4.03万
• 依托单位: Niigata University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07457351/
• 关键词: adult rat; descending pain control; spinal cord slice; substantia gelatinosa; serotonine; noradrenaline; acetylcholine; whole cell patch clamp; 下行性疼痛抑制路; ムスカリンレセプター

Whole cell patch clamp recordings were made from substantia gelatinosa (SG) neurons to investigate the mechanisms of descending pain control systems in spinal cord level.In the majority of SG neurons, serotonine (5-HT), noradrenaline (NA) and acetylcholine (ACh) evoked outward currents by increasing potassium conductance. These effects were mediated by 5-HTIA alpha2 and muscarinic receptors, respectively.NA,5-HT and ACh increased the frequency of spontaneous IPSCs. The effects of NA and ACh were mediated by alpha1 and muscarimic receptors, respectively. The receptor subtype involed in 5-HT action was not clear. These effects were partially blocked by TTX,especially large amplitude of IPSCs were eliminated by TTX,suggesting that a portion of these IPSCs were results from soma-dendritic spiking.NA,5-HT and ACh increased the frequency of miniature-IPSCs in the presence of TTX,suggesting thar NA,5-HT and ACh increase inhibitory transmitter release through presynaptic mechanism. The effects of NA and ACh were mediated by alpha1 and muscarinic receptors, respectively. The receptor subtype involved in 5-HT action was not clear.These results indicate that there are at least two mechanisms by which the descending pain control systems inhibit SG neuros : 1) hyperpolarization through activation of K conductance ; 2) activation of GABA and glycine-containing inhibitory interneurons through pre-and postsynaptic mechanisms.

对胶质质 (SG) 神经元进行全细胞膜片钳记录，以研究脊髓水平的下行疼痛控制系统的机制。在大多数 SG 神经元中，血清素 (5-HT)、去甲肾上腺素 (NA) 和乙酰胆碱 (ACh) ）通过增加钾电导引起外向电流。这些作用分别由5-HTIA α2 和毒蕈碱受体介导。NA、5-HT 和ACh 增加了自发IPSC 的频率。 NA 和 ACh 的作用分别由 α1 和毒蕈受体介导。参与 5-HT 作用的受体亚型尚不清楚。这些效应被TTX部分阻断，特别是大量的IPSC被TTX消除，表明这些IPSC的一部分是体细胞树突尖峰的结果。NA、5-HT和ACh增加了微型IPSC存在的频率TTX，表明 NA、5-HT 和 ACh 通过突触前机制增加抑制性递质释放。 NA 和 ACh 的作用分别由 α1 和毒蕈碱受体介导。参与5-HT作用的受体亚型尚不清楚。这些结果表明，下行疼痛控制系统至少有两种抑制SG神经的机制：1）通过激活K电导实现超极化； 2）通过突触前和突触后机制激活GABA和含甘氨酸的抑制性中间神经元。

项目成果

Baba H,et al.: "Facilitation of GABA release by carbachol and neostigmine in substantia gelatinosa of rat spinal cord." Neurosci Res. (Suppl 20). S60 (1996)

Baba H 等人：“卡巴胆碱和新斯的明在大鼠脊髓胶质质中促进 GABA 释放。”

Baba H,et al.: "Noradrenaline alpha1 receptors facilitate inhibitory transmitter release in substantia gelatinosa of the spinal cord." Progress in Anesthetic Mechanism. 3 (Special Issue). 314-317 (1995)

Baba H 等人：“去甲肾上腺素 α1 受体促进脊髓胶质质中抑制性递质的释放。”

Baba H, et al.: "Noradrenaline al receptors facilitate inhibitory transmitter release in substantia gelatinosa of the spinal cord." Pain Research. 9. 129 (1994)

Baba H 等人：“去甲肾上腺素受体促进脊髓胶质质中抑制性递质的释放。”

Baba H,et al.: "Noradrenaline alpha1 receptors facilitate inhibitory transmitter release in substantia gelatinosa of the spinal cord." Neurosci Res. Suppl 19. S58 (1994)

Baba H 等人：“去甲肾上腺素 α1 受体促进脊髓胶质质中抑制性递质的释放。”

M.Yoshimura et al.: "Patch clamp analysis of serotonin action on substantia gelatinosa neurons in adult rat spinal cord" Pain Research. 10. 51-56 (1995)

M.Yoshimura 等人：“膜片钳分析血清素对成年大鼠脊髓胶质神经元的作用”疼痛研究。