Cell-Biological Study on Medicolegal Diagnosis of Injury

损伤法医学诊断的细胞生物学研究

• 批准号: 07457118
• 负责人: TSUJI Tsutomu
• 金额: $ 2.62万
• 依托单位: Wakayama Medical College
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07457118/
• 关键词: Injury; Cell Death; Apoptosis; Necrosis; TNF alpha; TNF-α; 細胞死機構

In this study, we investigated cell-biologically the cell death generating by injuries, on the basis of two mechanisms of the cell death, these are necrosis and apoptosis.To heat the liver of rts, a heat device having a built-in PID electron regulater system was newly developed by us. The liver of rats laparotomized under anesthesia was heated at 37ﾟC,50ﾟC and 65ﾟC for 5 min. with a heated needle connected to the device, and thereafter the rats were survived with in the given time. Appearance of the cell death in the tissue injured by heat was analyzed, in progress of the survival time, by means of staining of the tissue with hematoxylin-eosin (HE) and TUNEL method and agarosegel electrophoresis of DNA extracted from the tissue injured.Consequently, apoptosis appeared, in all heat temperatures, with bimodal pattern showing peak at about 2 and 10 hours after heat. Apoptosis in the first peak dottedly appeared, in heat temperature at 37ﾟC,in the whole region affected by heat. When the li … More ver was heated at 50ﾟC and 65ﾟC,it first appeared on outer edge in the region affected by heat, and then made progress to the center of the region, condensation of chromatin was, in HE staining, remarkable at border between the injured and normal tissues. These findings were different from those in 37ﾟC.It seems that apoptosis inthe first peak is caused by direct action of temperature. Apoptosis in the second peak, in all heat temperature, made progress from outer edge of the injured region to the center, time course of progression of the apoptosis was almost concurrent with that of infiltration of neutrophil to the injured region. Ladder formation was observed, when DNA extracted from the tissue showing apoptosis was applied for agarosegel electrophoresis. Necrosis in the injured tissue appeared in the same pattern with appearance of apoptosis in time course and on location, necrosis and apoptosis were observed in almost same degree up to appearance of the second peak of apoptosis. After 24 hours of heat, apoptosis cells were phagocytosed, only necrotic tissue remained. It seems that appearance of necrosis and apoptosis is caused by action of TNF alpha derived from neutrophils, since generation of TNF alpha was confirmed electron microscopically.In this study, it became cell-biologically evident that apoptosis is intensely concerned with cell death in the injury tissue. Less

在这项研究中，我们从生物学上研究了损伤产生的细胞死亡，基于细胞死亡的两种机制，它们是坏死和凋亡。为了加热RTS，我们新开发了带有内置PID电子调节器系统的热装置。在麻醉下的大鼠肝脏在37℃，50°C和65℃下加热5分钟。将加热的针连接到设备，然后在给定时间内与大鼠一起生存。通过用苏木精 - eosin（He）（He）和Tunel方法和TUNEL方法和琼脂糖电泳对从组织受伤的组织受伤提取的DNA的组织染色，在生存时间的进展中分析了细胞死亡的表现，在生存时间的进展过程中，出现了poptosis的DNA。在第一个峰中的凋亡在37°C的热温下，在受热量影响的整个区域中出现。当Li…在50°C和65 c上加热更多的VER时，它首先出现在受热影响的区域的外边缘上，然后在该区域的中心进展，染色质的凝结在受伤和正常组织之间的边界上很明显。这些发现与37°C中的发现不同。似乎第一个峰中的凋亡是由温度的直接作用引起的。在所有热温下，第二个峰的凋亡使从受伤区域的外边缘到中心的进展，凋亡的进展时间几乎是同时发生的，中性粒细胞浸润到受伤区域。当从显示凋亡的组织中提取DNA时，观察到梯子形成。受伤组织中的坏死以相同的模式出现，时间过程和位置凋亡的出现，在几乎相同的程度上观察到坏死和凋亡，与凋亡的第二个峰值出现。热量24小时后，吞噬细胞被吞噬，仅残留坏死组织。似乎是由嗜中性粒细胞衍生的TNFα的作用引起的坏死和凋亡的出现，因为以电子方式证实了TNFα的产生。在这项研究中，它成为生物学上的证据，表明细胞凋亡与损伤组织中细胞死亡积极有关。较少的