Experimental study on sudden death due to acute myocardial infarction.

急性心肌梗死猝死的实验研究。

• 批准号: 07457117
• 负责人: SUZUKI Tsuneo
• 金额: $ 4.74万
• 依托单位: Yamagata University School of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07457117/
• 关键词: Sudden death; ventricular fibrilation; Myocardial infarction; Luxol fast blue staining; ルクソ-ルファストブルー染色

To Investigate the relatlonshIp between sudden death and the time elapsed after the onset of myocardial infarction and to find a suitable staining method for detection of acute myocardial Infarction, an experimental model of acute myocardial ischemia was made with mongrel dogs by ligating anterior descending branch of left coronary artery. The heart was given electrical simulation at the vulnerable period of the cardiac cycle and the ventricular fibrillation threshold was estimated. Ventricular fibrillation threshold decreased to about 20 % of those of the control hearts 15-30 mm after the coronary artery ligation. Then ii increased with the time elapsed after the coronary ligation and showed almost same values as those of control hearts 4 h after the coronary ligation. The ischemic hearts were fixed with 10 % formalin solution. The sections from the ischemic heart muscle were stained with Luxol Fast Blue (LFB) solution for 24 h and were differentiated for 1-3 min in several changes of 75 % alcohol to a faint dove-colored blue (a newly modified LFB staining). The newly modified LFB staining revealed the myocardial ischemic changes 15-30 min after the coronary ligation. The result suggested that sudden death from ventricular fibrillation is the aptest to occur 15-30 min after the onset of myocardial infarction and the newly modified LFB staining Is useful for detecting such very early myocardial infarction.

为探讨猝死与心肌梗死发病后时间的关系，寻找适合检测急性心肌梗死的染色方法，采用结扎左前降支的杂种犬制作急性心肌缺血实验模型。冠状动脉。在心动周期的易损期对心脏进行电模拟，估计室颤阈值。冠状动脉结扎后 15-30 毫米，心室颤动阈值降低至对照心脏的约 20%。然后，ii随着冠状动脉结扎后时间的推移而增加，并在冠状动脉结扎后4小时显示出与对照心脏几乎相同的值。将缺血的心脏用10％福尔马林溶液固定。将缺血心肌切片用 Luxol 固蓝 (LFB) 溶液染色 24 小时，并在 75% 酒精中多次变化为淡鸽色蓝色（新修改的 LFB 染色），分化 1-3 分钟。新改良的 LFB 染色显示冠状动脉结扎后 15-30 分钟的心肌缺血变化。结果表明，室颤猝死最容易发生在心肌梗塞发作后15-30分钟，新改良的LFB染色可用于检测这种极早期的心肌梗塞。

项目成果

大内孝幸 他: "心筋梗塞による突然死の実験的モデル" 日本法医学雑誌. 49(補冊). 150 (1995)

Takayuki Ouchi 等：“心肌梗塞引起的猝死的实验模型”，日本法医学杂志 49（增刊）150（1995）。

T.Ouchi,T.Suzuki et al.: "A newly staining for myocardial in farction" Advances Legal Med.3. 411-412 (1996)

T.Ouchi、T.Suzuki 等人：“梗死心肌的新染色”Advanced Legal Med.3。

T.Ouchi, T.Suzuki et al.: "An experimental study on sudden death from myocardial infarction" Jap.J.Legal Med.(1998)

T.Ouchi、T.Suzuki 等：“心肌梗塞猝死的实验研究”Jap.J.Legal Med.(1998)

Tsuneo Suzuki: "A new histochemical staining for diagnosis of early myocardial damage" Forensic Sci.Internat.(1997)

Tsuneo Suzuki：“诊断早期心肌损伤的新组织化学染色”Forensic Sc​​i.Internat.(1997)

大内孝幸、 鈴木庸夫 他: "心筋虚血による突然死に関する実験的研究" 日法医誌. (発表予定). (1998)

Takayuki Ouchi、Tsuneo Suzuki 等：“心肌缺血引起的猝死的实验研究”日本法医学杂志（待出版）。