Experimental study on sudden death due to acute myocardial infarction.

急性心肌梗死猝死的实验研究。

• 批准号: 07457117
• 负责人: SUZUKI Tsuneo
• 金额: $ 4.74万
• 依托单位: Yamagata University School of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07457117/
• 关键词: Sudden death; ventricular fibrilation; Myocardial infarction; Luxol fast blue staining; ルクソ-ルファストブルー染色

To Investigate the relatlonshIp between sudden death and the time elapsed after the onset of myocardial infarction and to find a suitable staining method for detection of acute myocardial Infarction, an experimental model of acute myocardial ischemia was made with mongrel dogs by ligating anterior descending branch of left coronary artery. The heart was given electrical simulation at the vulnerable period of the cardiac cycle and the ventricular fibrillation threshold was estimated. Ventricular fibrillation threshold decreased to about 20 % of those of the control hearts 15-30 mm after the coronary artery ligation. Then ii increased with the time elapsed after the coronary ligation and showed almost same values as those of control hearts 4 h after the coronary ligation. The ischemic hearts were fixed with 10 % formalin solution. The sections from the ischemic heart muscle were stained with Luxol Fast Blue (LFB) solution for 24 h and were differentiated for 1-3 min in several changes of 75 % alcohol to a faint dove-colored blue (a newly modified LFB staining). The newly modified LFB staining revealed the myocardial ischemic changes 15-30 min after the coronary ligation. The result suggested that sudden death from ventricular fibrillation is the aptest to occur 15-30 min after the onset of myocardial infarction and the newly modified LFB staining Is useful for detecting such very early myocardial infarction.

为了调查心肌梗塞发作后猝死与经过的时间之间的关系，并找到一种检测急性心肌梗死的染色方法，通过与杂种犬的左前降冠状动脉分支相关，急性心肌缺血的实验模型是用杂种狗制成的。在心脏周期的脆弱时期给予心脏模拟，并估计心室纤颤阈值。冠状动脉连接后15-30 mm的对照心脏的心室纤颤阈值降低到约20％。然后，II随冠状动脉连接后经过的时间增加，并且在冠状动脉连接后4小时显示出与对照心脏的值几乎相同的值。缺血性心脏用10％的福尔马林溶液固定。将缺血性心肌的切片用卢克斯快蓝色（LFB）溶液染色24小时，并在几种变化的75％酒精中分化为1-3分钟，以变成微弱的鸽子色蓝色（一种新修饰的LFB染色）。新修饰的LFB染色显示冠状动脉连接后15-30分钟的心肌缺血变化。结果表明，心肌梗死发作后15-30分钟发生的最恰当发生的突然死亡，而新修饰的LFB染色对于检测如此早期的心肌梗塞很有用。

项目成果

大内孝幸 他: "心筋梗塞による突然死の実験的モデル" 日本法医学雑誌. 49(補冊). 150 (1995)

Takayuki Ouchi 等：“心肌梗塞引起的猝死的实验模型”，日本法医学杂志 49（增刊）150（1995）。

T.Ouchi,T.Suzuki et al.: "A newly staining for myocardial in farction" Advances Legal Med.3. 411-412 (1996)

T.Ouchi、T.Suzuki 等人：“梗死心肌的新染色”Advanced Legal Med.3。

T.Ouchi, T.Suzuki et al.: "An experimental study on sudden death from myocardial infarction" Jap.J.Legal Med.(1998)

T.Ouchi、T.Suzuki 等：“心肌梗塞猝死的实验研究”Jap.J.Legal Med.(1998)

Tsuneo Suzuki: "A new histochemical staining for diagnosis of early myocardial damage" Forensic Sci.Internat.(1997)

Tsuneo Suzuki：“诊断早期心肌损伤的新组织化学染色”Forensic Sc​​i.Internat.(1997)

大内孝幸、 鈴木庸夫 他: "心筋虚血による突然死に関する実験的研究" 日法医誌. (発表予定). (1998)

Takayuki Ouchi、Tsuneo Suzuki 等：“心肌缺血引起的猝死的实验研究”日本法医学杂志（待出版）。