Novel Molecular Imprinting by Latex Polymerization-Basic Research for the Structure of Resin Surface-

乳胶聚合新型分子印迹-树脂表面结构的基础研究-

基本信息

批准号：
07405038
负责人：
TAKAGI Makoto
金额：
$ 13.06万
依托单位：
KYUSHU UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (A)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1998
项目状态：
已结题

项目摘要

In recent years, the research area of design and synthesis of artificial host molecules is expanding focused by molecular recognition. We have developed a new method of molecular imprinting which produce a recognition site on the surface of latex particle.We started with a basic investigation of a system without template to connect the condition of emulsion and the structure of latex surface. The broadening of pKa was observed from proton titration of the resin. This result suggests the strong interaction between the functional groups and it is evidence that the carboxyl groups exist two dimensionally.We chose amino acid-derivatives as cationic organic template and synthesized imprinted resin. L-Phe-imprinted resin had high ability for adsorption of L-Phe. This result suggests the specific recognition site for L-Phe on the surface of the resin.We had used oleic acid as a functional surfactant, but there was possibility of missing of oleic acid from the resin. Then we designed and synthesized a new functional monomer possessing vinyl group. The form and character of the new resin was obviously different from the case of oleic acid. It is unknown what occurs during the surfactant was polymerized in the emulsion, but it was an interesting phenomenon.We tried an application to the membrane electrode-type ion sensor as a method for evaluation of imprinting effect. Molecular imprinted membrane was prepared by the polymerization after permeation of the salt of-functional monomer and organic cation into the membrane. This ion sensing membrane effectively responded to the templated ion, but it was not perfect Nernstian response.

近年来，人工宿主分子的设计和合成研究领域正在扩大以分子识别为重点。我们已经开发了一种新的分子印迹方法，该方法在乳胶粒子的表面上产生识别位点。我们从对系统的基本研究开始，没有模板，以连接乳液状况和乳胶表面的结构。从树脂的质子滴定中观察到了PKA的扩大。该结果表明官能团之间存在很强的相互作用，并且证据表明羧基在二维上存在。我们选择氨基酸衍生物作为阳离子有机模板和合成的印迹树脂。 L-Phe刻有树脂具有高吸附L-Phe的能力。该结果表明在树脂表面具有L-PHE的特定识别位点。我们使用油酸作为功能性表面活性剂，但可能会缺少树脂中的油酸。然后，我们设计并合成了具有乙烯基基团的新功能单体。新树脂的形式和特征显然与油酸的情况不同。在表面活性剂期间发生的情况是未知的，在乳液中是聚合的，但这是一个有趣的现象。我们尝试将膜电极型离子传感器应用于评估印迹效应的方法。分子印记的膜是通过聚合渗透到膜中的盐和有机阳离子后的聚合来制备的。这种离子传感膜有效地响应了模板的离子，但它并不是完美的nernstian响应。