Neuronal control of Cerebrovascular Bed

脑血管床的神经元控制

基本信息

批准号：
06671420
负责人：
MAEDA Minoru
金额：
$ 1.41万
依托单位：
JUNTENDO UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1994
资助国家：
日本
起止时间：
1994 至 1996
项目状态：
已结题

项目摘要

There are two possible causes for the development of plateau waves : persistent intracranial hypertension and cerebral vasomotor reaction. Previously we investigated the function of noradrenergic cell group (locus coeruleus complex, LC ; medullary reticular formation, MRF) and the cholinoceptive pontine area (CPA) in the generation of plateau waves. This study investigated the function of the cholinergic basal forebrain in control of ICP and cerebrovascular bed.Ten chloralose-anesthetized-immobilized cats with Kaolin-induced hydrocephalus and six normal rats were used of the experiments. BP,ICP and ETCO_2 were measured continuously. The CBF was measured by the hydrogen clearance method. The first experiments recorded single unit spikes continuously during ICP・variations using tungsten-microelectrode in the dorsomedial hypothalamic nucleus (DMH) of the cats. In the second series of experiments injection needles were inserted for microinjection of glutamate or Ach into the magnocellular … More basal nucleus (nucleus basalis of Meynert, NBM ; substantia innominata, SI) of the rats or the DMH of the cats.Glutamate or Ach microinjection into the NBM and SI caused consistent increases in ICP associated with slightly decreased BP,Ach microinjection into the DMH produced a persistent increase in ICP with a marked decrease in BP.ETCO_2 concentration changed little. Ach microinjection into the NBM or the DMH also induced consistent increases in CBF in the cerebral cortex. The DMH-neuron spike activities increased before onset and during spontaneous ICP elevation. The firing rate of the DMH-neuron discharge was increased in phase with the plateau wave like ICP-variations elicited by microinjection of Ach into the CPA or the contralateral DMH.However, some DMH-neurons demonstrated no suppression or promotion of spike activities during increased ICP.Plateau waves may result from an increase in CBV.Glutamate or Ach induced ICP changes in the present study have resulted from increased CBV in response to reduced cerebral vaso-constrictor tone. Our previous and present results suggest that activity within the cholinergic basal forebrain (NBM,SI,DMH), CPA,LC and MRF contributes to ICP changes and is the intrinsic neuronal origins controlling cerebrovascular bed. Less

高原波的发展有两个可能的原因：持续的颅内高血压和脑血管肌舒张症反应。以前，我们研究了去甲肾上腺素能细胞基（locus coeruleus complex，LC；髓质网状形成，MRF）和胆碱感知丘脑区域（CPA）在高原波的产生中的功能。这项研究研究了胆碱能基本前脑在控制ICP和脑血管床的功能。用高岭土诱导的脑积水的氯糖 - 麻醉的 - 毫米毫米化的猫，实验使用了六只正常大鼠。连续测量BP，ICP和ETCO_2。 CBF通过氢清除方法测量。第一个实验在ICP变化过程中使用tungsten-microelectrode在猫的背侧下丘脑核（DMH）中连续记录了单个单位尖峰。在第二系列的实验中，将注射针插入以将谷氨酸或ACH对磁细胞进行显微注射……更碱性的核（Meynert的基底核（Meynert的基底核），NBM； insia innominata，si，si，si）或与cats.glutamate或aCh aCh aCh的dmh相关的nbm和ic a in aCh iC降低，IC与nbm的ANH IC相关。对DMH的显微注射会导致ICP持续增加，而BP.ETCO_2浓度显着降低很小。 ACH对NBM或DMH的ACH显微注射也会引起大脑皮层中CBF的一致性增加。发作前和自发的ICP高程期间，DMH-Neuron尖峰活动增加。 DMH-神经元放电的发射速率与高原波相同，例如通过将ACH的ACH微注射引起的ICP差异提高，或者在对侧DMH中引起的发射速率。无论如何，某些DMH-神经元在ICP.Plateau Wave中的增加可能导致升高或促进峰值的升高或促进CAM的升高可能会导致ICB。由于CBV的增加而导致大脑血管收缩张张力降低而产生。我们以前的和现在的结果表明，胆碱能基本前脑（NBM，SI，DMH），CPA，LC和MRF内的活性有助于ICP变化，并且是控制脑血管床的固有神经元起源。较少的