Molecular ecology for the purpose of terminating toxic dinoflagellate by gene analysis of some enzymes relating to PSP toxin synethesis.

分子生态学，目的是通过对一些与 PSP 毒素合成相关的酶进行基因分析来终止有毒甲藻。

• 批准号: 04404014
• 负责人: ISHIDA Yuzaburo
• 金额: $ 18.56万
• 依托单位: KYOTO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (A)
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-04404014/
• 关键词: Paralytic shellfish poisoning; dinoflagellate; O-sulfotransferase; Mendelian inheritance; toxin profiles; G.catenatum; N-sulfotransferase; 麻痺性貝毒; アレキサンドリウム属; ギムノディニウム属; 有毒渦鞭毛藻; Alexandrium属; 粗酵素液; GTX欠損突然変異株; DNA抽出法; メンデルの法則

We investigated the stability of paralytic shellfish poisoning (PSP) toxin composition in the dinoflagellate Alexandrium catenella and A.tamarense, and the genetic system producing it. Using clonal axnic cultures, toxin compositions were determined in cell material from different growth phases of many populations and F_1progenies. In all isolates used, the mole percentage of toxin composition did not significantly change in any growth phase. Toxin composition analysis of over 70 strains of both species from 8 different waters indicate the occurrence of inter-and intra-specific indigenous populations from distant localities. Toxin compositions of many F_1progenies from crosses between algal strains having different toxin profiles were determined. Toxin compositions were inherited mostly in a 1 : 1 Mendelian pattern and sexuality (mating type) was not associated with toxin inheritance. These data strongly suggest that PSP toxin compositions of A.catenella and A.tamarense are stable and that Mendelian inheritance of toxin profiles occurs in both these heterothallic dinoflagellates.Furthermore we have tried to isolate some enzymes relating to PSP biosynthesis in A.tamarense and G.catenatum. Firstly, N-sulfotransferase from STX or GTX2+3 to GTX5 or C1+2 was purified from G.catenatum through several columns. The enzyme consisted of two identical subunits (63kDa) and was optmally active at pH6.0 and 25ﾟC.The activity was stimulated by Mg^<2+> and inhibited by PAPS analogue. Secondly, O-sulfotransferase which converted 11-alpha, beta -hydroxy STX into GTX2+3 was partially purified. DNA sequence of these enzymes will be analyzed in the coming year.

我们研究了在所有使用的遗传系统中，在遗传系统中，瘫痪的贝类中毒（PSP）组成。在8种不同的水域中，远处的毒素组合的特定土著种群的发生。 A.tamarene和A. tamarene的PSP毒素组成是稳定的，并且在两个这些异质Ellates中都会发生毒素谱 - 从STX或GTX2+3到GTX5或C1+2的硫化酶通过几个列（63KDA）纯化。 +>通过PAPS类似物抑制。

项目成果

Y.Ishida: "Toxic Phytoplankton Blooms in the Sea" Elsevier, 952 (1993)

Y.Ishida：“有毒浮游植物在海中大量繁殖” Elsevier，952 (1993)

左子 芳彦,金 昌勲,石田 祐三郎: "麻痺性貝毒の起源-海産渦鞭毛藻Alexandrium属" 化学と生物. 30. 726-734 (1992)

Yoshihiko Sako、Chang-hoon Kim、Yuzaburo Ishida：“麻痹性贝类毒素的起源 - 亚历山大藻属海洋甲藻”化学与生物学 30. 726-734 (1992)。

左子 芳彦,足立 真佐雄,石田 祐三郎: "微生物の生態18-微生物生態学の新たな展開と手法アイソザイム,モノクローナル抗体法による微細藻の識別" 学会出版センター, 151 (1992)

Yoshihiko Sako、Masao Adachi、Yuzaburo Ishida：“微生物生态学 18 - 微生物生态学的新进展以及使用同工酶和单克隆抗体方法鉴定微藻” Gakkai 出版中心，151（1992）

C.H.Kim: "Comparison of toxin composition between populations of Alexandrium spp.from geographically distant areas" Nippon Suisan Gakkaishi. 59. 641-646 (1993)

C.H.Kim：“来自地理遥远地区的亚历山大藻种群之间的毒素成分比较”Nippon Suisan Gakkaishi。

Y.Sako: "Harmful Marine Algal Blooms" Lavoisier, 878 (1995)

Y.Sako：“有害的海洋藻华”拉瓦锡，878 (1995)