A development of repetitious mechanical stress loading system for cultured cells.

培养细胞重复机械应力加载系统的开发。

基本信息

批准号：
03557078
负责人：
YAMADA Shoji
金额：
$ 4.03万
依托单位：
Showa University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Developmental Scientific Research (B)
财政年份：
1991
资助国家：
日本
起止时间：
1991 至 1993
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-03557078/
关键词：
OSTEOBLAST PERIODONTAL LIGAMENT CELL CULTURE MECHANICAL STRESS REPETITIOUS STRETCH CELL DIVISION COLLAGEN GEL ALKALINE PHOSPHATASE コラ-ゲンゲル

项目摘要

At the start of project, we obtained the basic dotards for an appropnate strenght and cycle of mechanical stress to load on bone cells using our hand-made stress loading system. During an endurance test of parts, we realized that the solenoid driving system is not durable in CO_2 incubator, and we change the system to waterproof motor driving-cam transmitting system. This change brought some merits besides a durability. An accelerating process in reciprocating movement became controllable with changing a shape of cam, which was impossible for solenoid driving system. A manufacture for trial and reform were repeated for a year and the system was completed as a machine with features,1. A repetitious mechanical stress is loaded for cells embedded in thin layr of collagen gel by stretching a nylon mesh, which is a reinforcement for cllagen gel. Not only a distance of stretch and cycle but also an accelerating process in reciprocating movement is controllable with changing the speed of motor and the shape of cam.2. A collagen gel layr is thin enough (about 1 mm) to neglect the effects of increasing permeability of nutrition into the gel according a repetitious stretch. Moreover, you can set a sample for cyclic control, which moves in the medium reciprocally without any stretch, or a sample for stable control, which standstill in the medium. These features make it possible to distinguish the true effect of mechanical forth.3. A direct microscopic observation of cells in collagen gel layr during the load of stress is possible. This makes it possible to measure the amount of forth loaded on cells as an amount of deformation of cells, and to compare the data obtained from different machines.It was confirmed by an endurance test and two years use for test that this system has a good quality.

在项目开始时，我们获得了用于使用手工制造的应力加载系统在骨细胞上载荷的机械应力和机械应力周期的基本dotard。在对零件的耐力测试中，我们意识到螺线管驾驶系统在CO_2孵化器中不持久，并且我们将系统更改为防水电动机驾驶型电脑传输系统。这种变化除了耐用性外还带来了一些优点。通过改变凸轮的形状，往复运动的加速过程变得可以控制，这对于螺线管驾驶系统是不可能的。重复了一年的试用和改革制造商，该系统作为具有功能的机器完成，1。通过拉伸尼龙网嵌入胶原蛋白凝胶中的细胞，加载了重复的机械应力，这是Cllagen凝胶的增强。不仅可以通过改变电动机的速度和CAM的形状2。胶原蛋白凝胶Layr足够薄（约1毫米），可以忽略将营养渗透性增加到凝胶中的影响。此外，您可以为循环控制设置一个样品，该样品无需任何伸展而在培养基中移动，或者是稳定控制的样品，该样本在培养基中停滞不前。这些功能使区分机械的真实效果3。在应激载荷过程中，对胶原蛋白凝胶Layr中细胞的直接显微镜观察是可能的。这使得可以测量细胞上加载的第四次数量作为单元格的变形量，并比较从不同机器获得的数据。通过耐力测试证实了该系统的确认，并使用两年的测试使用该系统具有良好的质量。