A development of repetitious mechanical stress loading system for cultured cells.

培养细胞重复机械应力加载系统的开发。

基本信息

批准号：
03557078
负责人：
YAMADA Shoji
金额：
$ 4.03万
依托单位：
Showa University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Developmental Scientific Research (B)
财政年份：
1991
资助国家：
日本
起止时间：
1991 至 1993
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-03557078/
关键词：
OSTEOBLAST PERIODONTAL LIGAMENT CELL CULTURE MECHANICAL STRESS REPETITIOUS STRETCH CELL DIVISION COLLAGEN GEL ALKALINE PHOSPHATASE コラ-ゲンゲル

项目摘要

At the start of project, we obtained the basic dotards for an appropnate strenght and cycle of mechanical stress to load on bone cells using our hand-made stress loading system. During an endurance test of parts, we realized that the solenoid driving system is not durable in CO_2 incubator, and we change the system to waterproof motor driving-cam transmitting system. This change brought some merits besides a durability. An accelerating process in reciprocating movement became controllable with changing a shape of cam, which was impossible for solenoid driving system. A manufacture for trial and reform were repeated for a year and the system was completed as a machine with features,1. A repetitious mechanical stress is loaded for cells embedded in thin layr of collagen gel by stretching a nylon mesh, which is a reinforcement for cllagen gel. Not only a distance of stretch and cycle but also an accelerating process in reciprocating movement is controllable with changing the speed of motor and the shape of cam.2. A collagen gel layr is thin enough (about 1 mm) to neglect the effects of increasing permeability of nutrition into the gel according a repetitious stretch. Moreover, you can set a sample for cyclic control, which moves in the medium reciprocally without any stretch, or a sample for stable control, which standstill in the medium. These features make it possible to distinguish the true effect of mechanical forth.3. A direct microscopic observation of cells in collagen gel layr during the load of stress is possible. This makes it possible to measure the amount of forth loaded on cells as an amount of deformation of cells, and to compare the data obtained from different machines.It was confirmed by an endurance test and two years use for test that this system has a good quality.

在项目开始时，我们获得了使用我们手工制作的应力加载系统对骨细胞施加适当强度和机械应力周期的基本标准。在对零件进行耐久性测试时，我们发现电磁驱动系统在CO_2培养箱中不耐用，将系统改为防水电机驱动凸轮传动系统。除了耐用性之外，这种变化还带来了一些优点。通过改变凸轮的形状，可以控制往复运动的加速过程，而这对于螺线管驱动系统来说是不可能的。反复试制、改造一年，系统完成，具有以下特点： 1．通过拉伸尼龙网，对嵌入胶原蛋白凝胶薄层中的细胞施加重复的机械应力，尼龙网是胶原蛋白凝胶的增强物。通过改变电机的转速和凸轮的形状，不仅可以控制拉伸和循环的距离，还可以控制往复运动的加速过程。2．胶原蛋白凝胶层足够薄（约 1 毫米），可以忽略由于重复拉伸而增加营养物质渗透到凝胶中的影响。此外，您还可以设置循环控制样品（在介质中无任何拉伸地往复移动）或稳定控制样品（在介质中静止）。这些特征使辨别机械效果的真实效果成为可能。3．在应力负荷期间可以直接用显微镜观察胶原凝胶层中的细胞。这使得可以测量电池上的第四负载量作为电池的变形量，并比较从不同机器获得的数据。通过耐久性测试和两年的使用测试证实，该系统具有良好的性能。质量。