Studies on the transcriptional regulatory factors controlling the expression of hCG and LH gene

控制hCG和LH基因表达的转录调控因子的研究

• 批准号: 03670785
• 负责人: KATAYAMA Kazuaki
• 金额: $ 1.28万
• 依托单位: Kobe University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1991
• 资助国家: 日本
• 起止时间: 1991 至 1992
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-03670785/
• 关键词: hCGbeta gene; LHbeta gene; trophoblast; footprint assay; gel shift assay; hCGβ; 転写制御因子; LHβ

The hCGb subunit is encoded by a cluster of six genes (or pseudogenes). Linked to this cluster is the single gene encoding the related LHbeta subunit. The general structure of these genes is very similar. hCGbeta genes are expressed mainly in first trimester placenta and LHbeta gene is expressed in pituitary gland. In this project, we tried to elucidate what factors control the tissue and stage specific expression of hCGbeta gene and LHbeta gene. Promoter region of these genes were isolated and used for footprint assays and gel shift assays. Although the footprint protection pattern of the hCGbeta gene fragment and LHbeta gene fragment using trophoblastic nuclear extract was very similar, there was a region LHbeta was protected but hCGbeta was not protected. Gel shift assays showed the factor which binds to the region was expressed in tissues except pituitary. It suggests that the factor suppresses expression of LHbeta gene. In vitro mutagenesis to make LHbeta promoter mutants substituted with the hCGbeta nucleotide sequence was made to analyze key sequences to acquire activity in trophoblasts.We also performed footprint assays of hCGbeta gene using trophoblastic nuclear extracts from various stage of pregnancy. They did not show marked difference. Probably there are many nuclear factors binding to the same domain and it is hard to detect change of some factors.To know the difference of the active hCGbeta gene and inactive hCGbeta gene, upstream sequence of hCGbeta gene 5 and hCGbeta gene 7 were used for footprint assays. We could not detect visible change of the protection pattern. It suggests that there may be factors we cannot detect by footprint assay.The information obtained here provides a foundation for further studies on nuclear factors binding to this region regulating hCGbeta gene and LHbeta gene.

HCGB亚基由六个基因（或假基因）的簇编码。与该群集相关的是编码相关LHBETA亚基的单个基因。这些基因的一般结构非常相似。 HCGBETA基因主要在三个月胎盘中表达，而LHBETA基因在垂体中表达。在这个项目中，我们试图阐明哪些因素控制HCGBETA基因和LHBETA基因的组织和分期特定表达。这些基因的启动子区域被分离并用于足迹测定和凝胶转移测定。尽管使用滋养细胞核提取物的HCGBETA基因片段和LHBETA基因片段的足迹保护模式非常相似，但有一个LHBETA受到保护，但HCGBETA并未受到保护。凝胶转移测定法显示了与该区域结合的因子，在垂体以外的组织中表达。这表明该因子抑制了LHBETA基因的表达。在体外诱变使LHBETA启动子突变体用HCGBETA核苷酸序列取代，以分析关键序列以在滋养片剂中获取活性。我们还使用来自各个怀孕的各个阶段的Trophastic核提取物进行了HCGBETA基因的足迹测定。他们没有显示出明显的差异。可能有许多核因子与同一结构域结合，很难检测到某些因素的变化。要知道活性HCGBETA基因和无活跃的HCGBETA基因的差异，HCGBETA基因5和HCGBETA基因7的上游序列用于足迹分析。我们无法检测到保护模式的可见变化。这表明可能有一些因素我们无法通过足迹测定法检测到。此处获得的信息为进一步研究与该区域结合的核因子结合的基础为调节HCGBETA基因和LHBETA基因的结合提供了基础。

项目成果

Yamaguchi Y.et al: "Studies on Placental Alkaline Phosphatase Gene Expression" Adv Obstet Gynecol. 44. 239-244 (1992)

Yamaguchi Y.et al：“胎盘碱性磷酸酶基因表达的研究”Adv Obstet Gynecol。

大谷 徹郎,望月 眞人: "胎盤ホルモンの遺伝子ーhCG遺伝子を中心としてー" 産婦人科の実際. 40. 603-606 (1991)

Tetsuro Otani，Masato Mochizuki：“胎盘激素基因 - 关注 hCG 基因”妇产科实践 40. 603-606 (1991)。