Study of differention inducing factors on the differention of placental cells

分化诱导因子对胎盘细胞分化影响的研究

基本信息

批准号：
03670789
负责人：
HAYASHI Keiji
金额：
$ 1.34万
依托单位：
Kagawa Medical School
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1991
资助国家：
日本
起止时间：
1991 至 1993
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-03670789/
关键词：
Placenta Endometrium Tretinoin Phorbol ester PTHrP EGF hCG Retinoic acid Triiode thyronine

项目摘要

Placenta plays important roles during pregnancy to the fetus and mother. To the fetus, it tranports nutrients, oxigen and hormones from The mother. To the mother, it provides many hormones and changes metabolic status. Local environment in the reproductive organs, especially in the placenta and endometrum communicate each other, via locally produced growth factors, cytokaines and hormones, in the paracrine or autocrine manner. In this context, we examined how these substances modulates the biological functions of the trophoblastic cells as well as endometrial cells, and analyzedz the molecular characters of hormones or substances produced in the reproductive organs.First, we clarified the stimulatory effect of retinoic acid, known as differentiation inducer, and ascorbic acid on human chorionic gonadotropin, indispensable to maintain the early stage of pregnancy, produced in the trophoblastic cells.Second, production of parathyroid hormone-related hormone(PTHrP), known as the substance causing humoral hyper calcemia of malignancy, local regulator of calcium metabolism and growth factor, was demonstrated in the reproductive organs, especially in the trophoblastic cells. We also examined the blood PTHrP levels during pregnancy and in the fetus. The results suggest that PTHrP plays important roles to maintain the calcium homeostasis in the environment of mother and fetus.Third, how the biological function(plasminogen activator system)of endometrial cells is modulated was examined. We showed that phorbol ester, protein kinase C stimulator, and EGF stimulated the tissue-type plasminogen antivator production as well as plsminogen inhibitor-1 production at the pretranslational level in the endometrial cells.

胎盘在怀孕期间对胎儿和母亲起着重要作用。它从母亲那里向胎儿输送营养物质、氧气和激素。对于母亲来说，它提供了许多激素并改变了代谢状态。生殖器官的局部环境，特别是胎盘和子宫内膜，通过局部产生的生长因子、细胞因子和激素，以旁分泌或自分泌的方式相互沟通。在此背景下，我们研究了这些物质如何调节滋养层细胞以及子宫内膜细胞的生物学功能，并分析了生殖器官中产生的激素或物质的分子特征。首先，我们阐明了视黄酸的刺激作用，已知作为分化诱导剂，抗坏血酸对人绒毛膜促性腺激素是维持妊娠早期不可缺少的，在滋养层细胞中产生。二、甲状旁腺的产生激素相关激素(PTHrP)被认为是引起恶性肿瘤体液高钙血症的物质、钙代谢的局部调节剂和生长因子，在生殖器官，尤其是滋养层细胞中得到证实。我们还检查了怀孕期间和胎儿的血液 PTHrP 水平。结果表明PTHrP在维持母体和胎儿环境中的钙稳态中发挥着重要作用。第三，研究了子宫内膜细胞的生物学功能（纤溶酶原激活剂系统）是如何调节的。我们发现佛波酯、蛋白激酶 C 刺激剂和 EGF 可刺激子宫内膜细胞翻译前水平的组织型纤溶酶原抗剂产生以及纤溶酶原抑制剂 1 产生。