Coupling mechanism of mechano-sensing and cellular reactivity in the process of stretch activation of vascular tissue.

血管组织拉伸激活过程中机械传感和细胞反应的耦合机制。

• 批准号: 02671005
• 负责人: NAKAYAMA Koichi
• 金额: $ 1.47万
• 依托单位: Department of Pharmacology, School of Pharmaceutical Sciences, University of Shizuoka
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1990
• 资助国家: 日本
• 起止时间: 1990 至 1991
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-02671005/
• 关键词: Mechano-sensor; Calcium signaling; Calcium channel; Phospholipase C; Vascular tissue; Alpha-toxin; inositol 1, 4, 5 trisphosphate; Vasodilator; 脳血管; 筋原性収縮; 急速伸展刺激; 物理受容・応答; Ca^<2+>チャネル; Gー蛋白; ホスホリパ-ゼC; ライアノジン

The vascular system is a site of not only chemical but also mechanical reception, and these stimuli elicit subsequent cellular responses such as contraction and relaxation. The vascular tissues such as cerebral and coronary arteries and lymphaticus, which are particularly sensitive to stretch, possess structural similarities ; lowpitched muscular lining, well-developed caveola beneath the plasma membrane, and abundant mitochondria. The mechanical reception is more susceptible than pharmacological reception to chemical skinning, suggesting the importance of membrane lipids as a mechanosensor domain. Phospholipase C coupled to GTP-binding protein, possibly a G_q class, which is insensitive to cholera- or pertussis toxin, may play a role in the genesis of vascular tone produced by quick stretch. The vascular reactivity to a mechanical stimulus may be mediated through the pathways of Ca^<2+> signaling which are specific to the mechano-reception and common to a conventional vasoconstrictive agonist such as norepinephrine.

血管系统不仅是化学接收的场所，也是机械接收的场所，这些刺激会引发随后的细胞反应，例如收缩和松弛。脑动脉、冠状动脉和淋巴管等血管组织对拉伸特别敏感，具有结构相似性；低沉的肌肉衬里，质膜下方发育良好的小凹，以及丰富的线粒体。机械接收比药理学接收更容易受到化学结皮的影响，这表明膜脂作为机械传感器域的重要性。与 GTP 结合蛋白（可能是 G_q 类）偶联的磷脂酶 C，对霍乱或百日咳毒素不敏感，可能在快速拉伸产生的血管张力的形成中发挥作用。对机械刺激的血管反应性可以通过Ca 2+ 信号传导途径介导，该信号传导对于机械接收是特异性的并且对于常规血管收缩激动剂例如去甲肾上腺素是常见的。

项目成果

中山 貢一: "循環作用薬の探索 血管拡張薬・収縮薬 医薬品の開発9(2)第5章" 廣川書店、東京, 65-78 (1991)

Koichi Nakayama：“寻找循环药物：血管扩张剂/收缩剂药物开发 9(2) 第 5 章”广川书店，东京，65-78 (1991)

K.Nakayama Y.Tanaka: "Specific signal transduction in the stretch-induced tone of vascular tissue.In:Resistance Arteries,Structure and Function.Ed.by M.J.Mulvany,C.Aalkjaer,A.M.Heagerty.N.C.B.Nyborg & S.Strandgaard." Elsevier Science Publishers B.V.,Amste

K.Nakayama Y.Tanaka：“血管组织拉伸引起的张力中的特定信号转导。见：阻力动脉、结构和功能。M.J.Mulvany、C.Aalkjaer、A.M.Heagerty.N.C.B.Nyborg 编辑

Y.Tanaka K.Nakayama: "Responses of endothelium-intact and denuded feline and canine cerebral arteries to quick stretch." Asia Pacific J.Pharmacol.6. 159-163 (1991)

Y.Tanaka K.Nakayama：“内皮完整和裸露的猫科动物和犬科动物大脑动脉对快速拉伸的反应。”

K.Nakayama,Y.Ishigai,H.Uchida & Y.Tanaka: "Potentiation by endothelin-1 of 5-hydroxytryptamine-induced contraction in coronary artery of the pig." Br.J.Pharmacol.104. 978-986 (1991)

中山 K、石贝 Y、内田 H

中山 貢一: "ニソルジピン" 医薬ジャ-ナル、東京、大阪老化と疾患. 4. 119-124 (1991)

Koichi Nakayama：“尼索地平”制药杂志，东京，大阪衰老与疾病。 4. 119-124 (1991)