Local Factors in Bone Metabolism of Experimental Osteoporosis

实验性骨质疏松症骨代谢的局部因素

基本信息

批准号：
02670654
负责人：
OKUMURA Hideo
金额：
$ 1.54万
依托单位：
Ehime University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1990
资助国家：
日本
起止时间：
1990 至 1992
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-02670654/
关键词：
Osteoporosis Rat Northern blot analysis m-RNA Local factor Osteocalcin 骨芽細胞活性型ビタミンD_3 実験的骨粗鬆症ノ-ザンブロッティング法 Cーfos cーDNA mーRNA TGFーβ CーDNA

项目摘要

Human osteoporosis is induced by aging, menopause and immobilization. Various hormones which regulate calcium metabolism in the whole body and various local factors which regulate bone metabolism in local bony tissue affect on the pathophysiology of osteoporosis. The expression of mRNA of local factors in the bone cells of osteoporotic tissue induced by immobilization combined with ovariectomy was studied. The mRNA was analyzed by Northern blot method using cDNA probes. The osteocalcin mRNA level in the osteoporotic tibiae significantly increased. The TGF-beta mRNA level increased in bone marrow cells and slightly increased in bone cells in the osteoporotic tibiae. The experiment of administration of dexamethasone was also done. The osteocalcin mRNA level in the tibiae of the rats treated with dexamethasone significantly decreased. Suppressive effect of dexamethasone on osteocalcin expression was persistent for seven days at higher dose. Northern blot analysis showed that both 1,25(OH)_2D_3 and 2beta-(3-hydroxypropoxy)-1,25(OH)_2D_3 caused an increase in osteocalcin mRNA levels in bone. And they increased osteocalcin mRNA levels in osteoblast-like cells ( ROS17/2.8 ) in vitro. In conclusion, general factors ( estrogen, 1,25(OH)_2D_3 ) have an effect on the trascriptional regulation of local factors ( osteocalcin, TGF- beta ) in bone.

人类骨质疏松是由衰老，更年期和固定化引起的。调节全身钙代谢的各种激素和调节局部骨组织中骨代谢的各种局部因素会影响骨质疏松的病理生理学。研究了固定化与卵巢切除术联合诱导的骨质疏松组织骨细胞中局部因子的mRNA的表达。使用cDNA探针通过Northern印迹法分析mRNA。骨质疏松性胫骨中的骨钙素mRNA水平显着增加。骨髓细胞中TGF-βmRNA水平升高，骨质疏松性胫骨中的骨细胞中略有增加。也进行了地塞米松给药的实验。用地塞米松治疗的大鼠胫骨中的骨钙素mRNA水平显着降低。地塞米松对骨钙素表达的抑制作用持续7天，剂量较高。 Northern印迹分析表明，1,25（OH）_2D_3和2Beta-（3-羟基丙氧基）-1,25（OH）_2D_3均导致骨骼中骨钙钙蛋白mRNA水平的升高。它们在体外增加了成骨细胞样细胞（ROS17/2.8）中骨钙素mRNA水平。总之，一般因素（雌激素，1,25（OH）_2D_3）对骨骼中局部因素（骨钙素，TGF-β）的trascription调节产生影响。