Local Factors in Bone Metabolism of Experimental Osteoporosis

实验性骨质疏松症骨代谢的局部因素

基本信息

批准号：
02670654
负责人：
OKUMURA Hideo
金额：
$ 1.54万
依托单位：
Ehime University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1990
资助国家：
日本
起止时间：
1990 至 1992
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-02670654/
关键词：
Osteoporosis Rat Northern blot analysis m-RNA Local factor Osteocalcin 骨芽細胞活性型ビタミンD_3 実験的骨粗鬆症ノ-ザンブロッティング法 Cーfos cーDNA mーRNA TGFーβ CーDNA

项目摘要

Human osteoporosis is induced by aging, menopause and immobilization. Various hormones which regulate calcium metabolism in the whole body and various local factors which regulate bone metabolism in local bony tissue affect on the pathophysiology of osteoporosis. The expression of mRNA of local factors in the bone cells of osteoporotic tissue induced by immobilization combined with ovariectomy was studied. The mRNA was analyzed by Northern blot method using cDNA probes. The osteocalcin mRNA level in the osteoporotic tibiae significantly increased. The TGF-beta mRNA level increased in bone marrow cells and slightly increased in bone cells in the osteoporotic tibiae. The experiment of administration of dexamethasone was also done. The osteocalcin mRNA level in the tibiae of the rats treated with dexamethasone significantly decreased. Suppressive effect of dexamethasone on osteocalcin expression was persistent for seven days at higher dose. Northern blot analysis showed that both 1,25(OH)_2D_3 and 2beta-(3-hydroxypropoxy)-1,25(OH)_2D_3 caused an increase in osteocalcin mRNA levels in bone. And they increased osteocalcin mRNA levels in osteoblast-like cells ( ROS17/2.8 ) in vitro. In conclusion, general factors ( estrogen, 1,25(OH)_2D_3 ) have an effect on the trascriptional regulation of local factors ( osteocalcin, TGF- beta ) in bone.

人类骨质疏松症是由衰老、更年期和不动引起的。调节全身钙代谢的各种激素和调节局部骨组织骨代谢的各种局部因素均影响骨质疏松的病理生理。研究固定结合卵巢切除致骨质疏松组织骨细胞中局部因子mRNA的表达。使用cDNA探针通过Northern印迹法分析mRNA。骨质疏松胫骨中骨钙素 mRNA 水平显着升高。骨髓细胞中的 TGF-β mRNA 水平增加，骨质疏松胫骨骨细胞中的 TGF-β mRNA 水平略有增加。还进行了地塞米松给药实验。地塞米松治疗的大鼠胫骨中骨钙素 mRNA 水平显着降低。在较高剂量下，地塞米松对骨钙蛋白表达的抑制作用可持续 7 天。 Northern印迹分析显示，1,25(OH)_2D_3和2β-(3-羟基丙氧基)-1,25(OH)_2D_3均导致骨中骨钙素mRNA水平增加。他们在体外增加了成骨细胞样细胞 (ROS17/2.8) 中的骨钙素 mRNA 水平。总之，一般因素（雌激素、1,25(OH)_2D_3）对骨中局部因素（骨钙素、TGF-β）的转录调节有影响。