Renal arterioles in development of glomerular sclerosis.

肾小球硬化发展中的肾小动脉。

基本信息

批准号：
02670379
负责人：
KIMURA Kenjiro
金额：
$ 1.6万
依托单位：
University of Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1990
资助国家：
日本
起止时间：
1990 至 1992
项目状态：
已结题

项目摘要

The intraglomerular pressure has been suggested to play an important role in development of glomerulusclerosis. The systemic pressure and pre- and post-glomerular arterioles determine the glomerular pressure and thus functional abnormalities in renal arterioles might leads to glomerular damage. In the present study, we investigated changes in the renal arteriolar diameters in various experimental models and tried to clarify the relationship between arterioles and glomerular damage.First, we demonstrated that the changes in arteriolar diameters were parallel to the changes in renal function and that changes in arteriolar diameters during the procedure of casting was not significant. These findings show that we can use microvascular casts in the present study. In the spontaneously hypertensive rats, streptozotocin diabetic rats, subtotally nephrectomized rats and anti-glomerular basement membrane nephritis rats, we showed that the renal arteriolar diameters and the systemic blood pressur … More e were important factor for the development of glomerulusclerosis and arteriolar dysfunction augmented glomerular damage. However, in the intervention study with antihypertensive agents, non-hemodynamic factors were suggested to play an important role in development of glomerulusclerosis in addition to hemodynamic factors. Accordingly, integrated studies on the mechanisms of development of glomerulusclerosis are necessary in the future.In the present study, we developed techniques to investigate renal arterioles in vivo under the microscope using hydronephrotic rats. Using this model, we revealed that manidipine hydrochloride dilated the efferent arterioles as well as the afferent arterioles, which is consistent with our previous micropuncture study. In spontaneously hypertensive rats, we also investigated arteriolar responses to other calcium channel blockers, angiotensin converting enzyme inhibitors, beta-blockers et al. Now we are going to investigate arteriolar dysfunction in various experimental model using this model. Less

肾小球内压力被认为在肾小球硬化的发展中发挥重要作用，全身压力和肾小球前和肾小球后小动脉决定肾小球压力，因此肾小动脉的功能异常可能导致肾小球损伤。研究了各种实验模型中肾小动脉直径的变化，并试图阐明小动脉与肾小球损伤之间的关系。首先，我们证明了小动脉直径的变化直径与肾功能的变化平行，并且在铸型过程中小动脉直径的变化并不显着。这些结果表明，我们可以在本研究中使用微血管铸型来进行自发性高血压大鼠、链脲佐菌素糖尿病大鼠的肾大部切除。通过对大鼠和抗肾小球基底膜肾炎大鼠的研究，我们发现肾小动脉直径和全身血压是发生肾小球基底膜肾炎的重要因素。然而，在抗高血压药物的干预研究中，除了血流动力学因素外，非血流动力学因素在肾小球硬化的发生过程中也发挥着重要作用，因此，对肾小球硬化发生机制的综合研究。在本研究中，我们开发了使用肾积水大鼠在显微镜下研究体内肾小动脉的技术。在该模型中，我们发现盐酸马尼地平可以扩张传出小动脉和传入小动脉，这与我们之前在自发性高血压大鼠中的微穿刺研究一致，我们还研究了小动脉对其他钙通道阻滞剂、血管紧张素转换酶抑制剂、β的反应。 -blockers 等人现在将使用该模型研究各种实验模型中的动脉功能障碍。