Genetic research on the Ryukyuan subspecies of the Ayu

香鱼琉球亚种的遗传研究

基本信息

批准号：
02660202
负责人：
SHOKITA Shigemitsu
金额：
$ 0.9万
依托单位：
Dept. of Mar. Sci., Coll. of Sci., Univ. of the Ryukyus
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1990
资助国家：
日本
起止时间：
1990 至 1991
项目状态：
已结题

项目摘要

Ayu in the Ryukyu Islands was recently recognized as a new subspecies, Plecoglossus altivelis ryukyuensis. To examine genetic characteristics of this ryukyuan subspecies, we investigated and compared genetic variation of the both Ayu subspecies and some related osmeroid fishes by using allozyme and mitochondrial DNA (mtDNA) analyses.Allozyme analyses showed that the both Ayu subspecies were distantly related with other osmeroid fishes. Results of various phylogenetic analyses of allozyme data did not support a phylogenetic hypothesis by Howes and Sanford (1987) who proposed that the Ayu is closely related with Osmerus or Hypomesus.For mtDNA examination, We used the PCR-mediated sequencing method, which is a newly established method, instead of the restriction site polymorphism analysis. Effective procedures of DNA extraction were examined. Experimental conditions of PCR amplification and DNA sequencing were also examined in detail using primers which were designed for amplification of various segments in mtDNA. Results of these examinations showed that many segments in mtDNA could be amplified and sequenced successfully when appropiate experimental conditions were employed. Sequence data of a 453-base-pair segment, including a part of the NAD dehydrogenase 4 gene, the histidine tRNA gene, and a part of the serine tRNA gene, suggested a genetic relationship similar to that from allozyme data. We conclude that the PCR-mediated DNA sequencing will provide a quite powerful tool for further genetic studies of the Ayu as well as other organisms.

琉球群岛的香鱼最近被认定为一个新亚种：Plecoglossus altivelis ryukyuensis。为了研究这个琉球亚种的遗传特征，我们通过同种酶和线粒体DNA（mtDNA）分析，研究并比较了这两个香鱼亚种和一些相关的香鱼亚种的遗传变异。同种酶分析表明，这两个香鱼亚种与其他香鱼亚种有远亲关系。鱼。对等位酶数据的各种系统发育分析的结果并不支持Howes和Sanford (1987)提出的系统发育假说，他们提出香鱼与Osmerus或Hypomesus密切相关。对于mtDNA检查，我们使用PCR介导的测序方法，这是一种新建立的方法，代替限制性位点多态性分析。对 DNA 提取的有效程序进行了检查。还使用专为扩增 mtDNA 中各个片段而设计的引物详细检查了 PCR 扩增和 DNA 测序的实验条件。这些检查结果表明，当采用适当的实验条件时，mtDNA 中的许多片段都可以成功扩增和测序。 453 个碱基对片段的序列数据，包括 NAD 脱氢酶 4 基因的一部分、组氨酸 tRNA 基因和丝氨酸 tRNA 基因的一部分，表明与等位酶数据相似的遗传关系。我们的结论是，PCR 介导的 DNA 测序将为香鱼以及其他生物的进一步遗传学研究提供一个非常强大的工具。