Developmental study on trichloroethylene degradation by a bioreactor

生物反应器降解三氯乙烯的进展研究

基本信息

批准号：
02556014
负责人：
UCHIYAMA Hiroo
金额：
$ 3.14万
依托单位：
National Institute for Environmental Sciences
依托单位国家：
日本
项目类别：
Grant-in-Aid for Developmental Scientific Research (B)
财政年份：
1990
资助国家：
日本
起止时间：
1990 至 1992
项目状态：
已结题

项目摘要

1. Characterization of liquid-solid phase trichloroethylene (TCE) degradation by Methylocystis sp. M (strain M) immobilized in Ca alginate was studied to maximize the degradation rate. The results obtained were as follows. (1) In order to get the cells of strain M possessing high specific activity for TCE degradation, elimination of Cu (almost to zero) in the medium and harvesting the cells at the end of the log phase were very important. (2 ) Kinetics constants (V max and Ks) and optimum conditions for TCE degradation (pH, temperature, DO) by immobilized cells were cleared. (3) The half life of the degradability of strain M was relatively short, and which was caused by the toxicity of intermediates formed by TCE degradation in the cells. (4) Inactivated cells by the intracellular toxicity were reactivated by incubation with methane or methanol, suggesting that the immobilized cells was possible to be repeatedly used in the TCE degradation. (5) Under the optimum conditions obtained, semicontinuous TCE degradation in a 5L bioreactor showed that the total amount of approximately 13mg TCE could be degraded in 120h.2. We also studied on the gas-solid phase TCE degradation system by the immobilized cells. The gaseous TCE of 0.3ppm, which was fed continuous into the glass-column bioreactor, was almost completely degraded in about 50h. The maximum TCE concentration to be degraded by this reactor was 100ppm, suggesting that, as the gaseous TCE concentration exhausted from ducts of TCE treatment facilities was too high (300-500ppm), the gaseous TCE was necessary to be diluted and be applied to the bioreactor.

1。通过甲基囊藻sp。液溶 - 固定相（TCE）降解的表征。研究了固定在Ca藻酸盐中的M（菌株M），以最大化降解速率。获得的结果如下。（1）为了获得具有高特异性活性的菌株细胞以降解，消除培养基中的Cu（几乎零）并在日志阶段结束时收集细胞非常重要。（2）清除了动力学常数（V MAX和KS）和TCE降解的最佳条件（pH，温度，DO），被固定的细胞清除。（3）菌株M的降解性的一半寿命相对较短，这是由于细胞中TCE降解形成的中间体的毒性引起的。（4）通过与甲烷或甲醇孵育的细胞内毒性被灭活的细胞重新激活，这表明可以在TCE降解中反复使用固定的细胞。（5）在获得的最佳条件下，5L生物反应器中的半连续TCE降解表明，大约13mg TCE的总量可以在120h.2中降解。我们还研究了固定的细胞对气体固相TCE降解系统的研究。 0.3ppm的气体TCE连续喂入玻璃列生物反应器中，几乎完全降解了约50小时。该反应器要降解的最大TCE浓度为100ppm，这表明，由于TCE处理设施的管道耗尽的气态TCE浓度过高（300-500ppm），因此必须将气态稀释并应用于生物反应器。