Production of gene map using chromosome deletions in common wheat

利用普通小麦染色体缺失绘制基因图谱

基本信息

批准号：
02640499
负责人：
ENDO Takashi
金额：
$ 1.02万
依托单位：
Nara University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1990
资助国家：
日本
起止时间：
1990 至 1991
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-02640499/
关键词：
Common wheat Deletion mapping Deletion chromosome Gene map

项目摘要

The main purpose of this research was to produce a series of deletion stocks in a common wheat cultivar Chinese Spring, and it was mostly fulfilled by the study conducted from 1990 to 1991. A total of 382 deletion chromosomes were identified and isolated through the cytological selection by C-banding during several self-pollinated generations. 263 of them were in structually homozyhgous condition, in which there are a pair of a critical deletion and no corresponding normal homologue. These deletion stocks can be used for deletion mapping as they are. For the remaining deletions, however, homozygous plants were neither obtained nor fertile. Therefore, most of these heterozygous deletion stocks, in which there are one critical deletion and one corresponding normal homologue, were crossed with Chinese Spring nullisomic-tetrasomic stocks, which lack a pail, of chromosomes and have two pairs of a homoeologous chromosomes. From these Fl's, hemizygous plants, in which there is one critical deletion and no corresponding homologue, can be selected for deletion mapping. The deletions in the short arms of chromosome 2A and chromosoem 4B, whose homozygotes were sterile, have been maintained in heterozygou condition because no appropriate nullisomic-tetrasomics are available.Using the deletion stocks so far produced, deletion mappings with male-fertility gene and fertility-restoring gene against alien cytoplasm were conducted and the results wei, e published, or will be published soon. Other deletion mapping concerning some isozyrne genes and DNA markers are in progress.

本研究的主要目的是在普通小麦品种“中国春”中产生一系列缺失母系，1990年至1991年的研究大部分实现了这一目标。通过细胞学选择，共鉴定和分离了382条缺失染色体。在几个自花授粉世代中通过C显带。其中263个在结构上处于纯合状态，其中存在一对关键性缺失且没有相应的正常同源物。这些删除库存可以按原样用于删除映射。然而，对于其余的缺失，既没有获得纯合植物，也没有能育。因此，大多数杂合缺失种群（其中有一个关键缺失和一个相应的正常同源物）与中国春零体-四体种群杂交，后者缺乏一桶染色体，但有两对同源染色体。从这些F1中，可以选择其中存在一个关键缺失且没有相应同源物的半合子植物用于缺失作图。 2A和4B染色体短臂的缺失，其纯合子是不育的，由于没有合适的无效体-四体体，一直保持在杂合状态。利用目前产生的缺失库，进行雄性生育基因和生育力的缺失作图-针对外来细胞质恢复基因已进行，结果已发表或即将发表。关于一些异酶基因和 DNA 标记的其他缺失定位正在进行中。