Production of gene map using chromosome deletions in common wheat

利用普通小麦染色体缺失绘制基因图谱

基本信息

批准号：
02640499
负责人：
ENDO Takashi
金额：
$ 1.02万
依托单位：
Nara University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1990
资助国家：
日本
起止时间：
1990 至 1991
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-02640499/
关键词：
Common wheat Deletion mapping Deletion chromosome Gene map

项目摘要

The main purpose of this research was to produce a series of deletion stocks in a common wheat cultivar Chinese Spring, and it was mostly fulfilled by the study conducted from 1990 to 1991. A total of 382 deletion chromosomes were identified and isolated through the cytological selection by C-banding during several self-pollinated generations. 263 of them were in structually homozyhgous condition, in which there are a pair of a critical deletion and no corresponding normal homologue. These deletion stocks can be used for deletion mapping as they are. For the remaining deletions, however, homozygous plants were neither obtained nor fertile. Therefore, most of these heterozygous deletion stocks, in which there are one critical deletion and one corresponding normal homologue, were crossed with Chinese Spring nullisomic-tetrasomic stocks, which lack a pail, of chromosomes and have two pairs of a homoeologous chromosomes. From these Fl's, hemizygous plants, in which there is one critical deletion and no corresponding homologue, can be selected for deletion mapping. The deletions in the short arms of chromosome 2A and chromosoem 4B, whose homozygotes were sterile, have been maintained in heterozygou condition because no appropriate nullisomic-tetrasomics are available.Using the deletion stocks so far produced, deletion mappings with male-fertility gene and fertility-restoring gene against alien cytoplasm were conducted and the results wei, e published, or will be published soon. Other deletion mapping concerning some isozyrne genes and DNA markers are in progress.

这项研究的主要目的是在1990年至1991年进行的研究中生产一系列缺失的股票，并且大多数是通过在几个自授予的世代中通过C-Banding进行的细胞学选择来鉴定并分离出382个缺失染色体的研究。其中263个处于结构纯合条件下，其中有一对临界缺失，没有相应的正常同源物。这些删除库存可用于删除映射。但是，对于其余的缺失，既没有获得也不肥沃的纯合植物。因此，将大多数这些杂合缺失股与中国弹簧非异构体四质量库存交叉，其中有一种严重的缺失和一种相应的正常同源物，它们缺乏pail，染色体，并有两对同源染色体。从这些FL的半合基植物中，可以选择一个关键的缺失，没有相应的同源物来进行缺失映射。在纯合子是无菌的2a染色体和染色体4B短臂中的缺失，由于没有可用的缺失库存，因为没有适当的无效 - 四个库存。或将很快出版。有关某些异元基因和DNA标记的其他缺失映射正在进行中。