Tetralogy of Fallot, Cardiac Hypertrophy, Pulmonary Hypertension and Aomalies of Great Vessels in WKY/NCrj Rats

WKY/NCrj 大鼠的法洛四联症、心脏肥大、肺动脉高压和大血管异常

基本信息

批准号：
02807096
负责人：
HAMAOKA Kenji
金额：
$ 1.02万
依托单位：
Kyoto Prefectural University of Medicine
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1990
资助国家：
日本
起止时间：
1990 至 1991
项目状态：
已结题

项目摘要

We examined anatomically the hearts, lungs, and great vessels of 269 WKY/NCrj rats at three fetal and three neonatal stages. Severe pulmonary valve thickening was present in 16 and ventricular septal defects with overriding of the aorta in 15 of the 90 near-term fetuses and in 10 and nine, respectively, of the 79 neonates at 2-4 d of age. These abnormalities occurred together (tetralogy of Fallot) in seven of the near-term fetuses and in five of the neonates. A narrow pulmonary outflow tract was present in 55% of the fetuses and in 56% of the neonates. The wall of the pulmonary arterial branch was abnormally thick in 19% of the fetuses and in 26% of the neonates, most of which did not have septal defects. In about 80% of the fetuses, the middle latitudinal muscle bundle of the ventricular septum was not-continuous with the left ventricular free wall, but rather with the right ; after birth, it was discontinuous with both free walls. The heart wag abnormally heavy in 49% of the 79 neona … More tes. In about half of the heavy hearts, there were no septal defects or pulmonary valvular and arterial lesions. There were double aortic arches in four and right aortic arches in six of the total WKY fetuses and neonates ; the ductus arteriosus was abnormally small in 47% and the aorta was large in 51% of the nearterm fetuses. This constellation of congenital heart disease is genetic in origin, but altered by hemodynamics late in fetal life.We examined 78 fetuses on d 21 of gestation (G21) and 83 neonates on d 2 after birth (A2), which were first generation offsping of WKY rats mated with normal Wistar rats (Fl). In addition, we examined six groups of fetuses on d 19 of gestation (Gl9) : 65 Wistar rats, 111 WKY rats, 85 Fl, 100 Fl x Fl, 92Fl x Wistar, and 97 Fl x WKY progeny. These results suggest that cardiovascular anomalies of WKY rati are transmitted as autosomal recessive or incomplete autosomal dominant traits with an incomplete penetrance and variable expressivity or as polygenic traits. Chromosomal analysis of 31 WKY fetuses revealed no abberations specifically related to the development of cardiovascular malformations.A high-frequency transducer was used to determine the optimal parameters for visualizing the heart in 40 normal Wistar, 15 SHR, and 10 aorta-banded rats. The rats were 5 to 30 weeks old and weighed between 105 and 705 grams. Two-dimensional and M-mode views of the ventricles, atria, valves, and great arteries were obtained by placing the transducer beneath the rats through the left or right parasternal window in either the prone or the right decubitus positions, respectively. Echocardiography is useful for obtaining anatomical evaluation and quantitative measurements in the hearts of the rats. Less

我们对 269 只处于三个胎儿阶段和三个新生儿阶段的 WKY/NCrj 大鼠的心脏、肺和大血管进行了解剖学检查，其中 16 只大鼠存在严重的肺动脉瓣增厚，并且在 90 只近期大鼠中，有 15 只存在室间隔缺损并跨越主动脉。 79 名 2-4 日龄新生儿中，分别有 10 名和 9 名胎儿同时出现这些异常（四联症）。法洛氏症）在 7 个近期胎儿和 5 个新生儿中，55% 的胎儿和 56% 的新生儿存在狭窄的肺流出道。 19%的胎儿和26%的新生儿，其中大多数没有间隔缺损，大约80%的胎儿有间隔缺损。室间隔横肌束与左心室游离壁不连续，而与右心室游离壁不连续；出生后，79 例新生儿中 49% 的心脏摆动异常重。大约一半的重型心脏没有间隔缺损或肺瓣膜和动脉病变，其中 4 例有双主动脉弓，6 例有右主动脉弓。在所有 WKY 胎儿和新生儿中，47% 的胎儿的动脉导管异常小，51% 的近足月胎儿的主动脉异常大，这种先天性心脏病的起源是遗传性的，但在胎儿生命后期的血流动力学方面却有所不同。在妊娠第 21 天（G21）检查了 78 名胎儿，在出生后第 2 天检查了 83 名新生儿(A2)，它们是 WKY 大鼠与正常 Wistar 大鼠 (F1) 交配的第一代后代。此外，我们检查了妊娠第 19 天的六组胎儿 (G19)：65 只 Wistar 大鼠、111 只 WKY 大鼠、85 只 F1、 100 Fl x Fl、92Fl x Wistar 和 97 Fl x WKY 后代这些结果表明 WKY 的心血管异常率更高。作为常染色体隐性或不完全常染色体显性性状传播，具有不完全外显率和可变表达性，或者作为 31 个 WKY 胎儿的多基因性状传播，未发现与心血管畸形的发展特别相关的畸变。观察 40 只正常 Wistar 大鼠、15 只 SHR 大鼠和 10 只带主动脉带的大鼠的心脏可视化的最佳参数为 5 至 5 只。 30 周龄、体重在 105 至 705 克之间的心室、心房、瓣膜和大动脉的二维和 M 模式视图是通过将传感器置于俯卧位的左侧或右侧胸骨旁窗口下方获得的。或右侧卧位，超声心动图可用于获得大鼠心脏的解剖学评估和定量测量。