Microcirculatory Disturbances in Cerebral Ischemia ; Analysis of Pathophysiology and Therapeutic Approach.

脑缺血时的微循环障碍；

基本信息

批准号：
01570810
负责人：
YOSHIMINE Toshiki
金额：
$ 1.15万
依托单位：
Osaka University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1989
资助国家：
日本
起止时间：
1989 至 1990
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-01570810/
关键词：
Cerebral ischemia Microcirculation Red blood cell Platelet White blood cell Superoxide 血漿高血圧 SHRSP

项目摘要

1. Progression of Microcirculatory Disturbance in Focal Cerebral Ischemia.Changes in the regional cerebral blood flow (rCBF) and the length of plasma-perfused microvessels were studied after occlusion of the middle cerebral artery (MCA) in rats or the posterior communicating artery (P-com) in gerbils. The data demonstrated that the rCBF decreased markedly within several minutes after arterial occlusion, while the disturbances in microvascular plasma perfusion appeared 30 min or 1 hour later which further deteriorated for the following several hours.2. Therapeutic approach to microvascular deterioration.To investigate the effects of tissue-type plasminogen activator (tPA) and Cu, Zn-superoxide dismutase (SOD) on the microvascular deterioration during cerebral ischemia, recombinant human tPA and SOD were continuously applied in rat MCA occlusion model. The results indicated that intravenous application of those enzymes significantly improved the microvascular plasma circulation.3. Microcirculation in Hypertensive Rats.The microvascular length per unit volume was measured in the strokeprone spontaneously hypertensive Rats (SHRSP) and the age-matched Wistar Kyoto rats. The data demonstarted that the microvascular length was decreased in several areas of the brain in SHRSP. Daily administrattion of elastase prepartion improved the microvascular plasma perfusion in SHRSP.

1.局灶性脑缺血微循环障碍的进展。研究大鼠大脑中动脉（MCA）或后交通动脉（P）闭塞后局部脑血流量（rCBF）和血浆灌注微血管长度的变化。 -com）在沙鼠中。数据显示，动脉闭塞后几分钟内rCBF明显下降，而微血管血浆灌注在30分钟或1小时后出现紊乱，并在随后的几个小时内进一步恶化。2．微血管恶化的治疗方法为了研究组织型纤溶酶原激活剂（tPA）和铜、锌超氧化物歧化酶（SOD）对脑缺血时微血管恶化的影响，将重组人tPA和SOD持续应用于大鼠MCA闭塞模型。结果表明，静脉注射这些酶可显着改善微血管血浆循环。3．高血压大鼠的微循环。测量易发生中风的自发性高血压大鼠(SHRSP)和年龄匹配的Wistar京都大鼠的每单位体积的微血管长度。数据表明，SHRSP 中大脑多个区域的微血管长度缩短。每日给予弹性蛋白酶制剂改善了 SHRSP 的微血管血浆灌注。