Development of a Schlieren Optics for Real-Time Visualization of Polyacrylamide Gel Electrophoresis

开发用于聚丙烯酰胺凝胶电泳实时可视化的纹影光学器件

基本信息

批准号：
63880019
负责人：
TAKAGI Toshio
金额：
$ 6.34万
依托单位：
Osaka University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Developmental Scientific Research (B).
财政年份：
1988
资助国家：
日本
起止时间：
1988 至 1990
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-63880019/
关键词：
Polyacrylamide Gel Electrophoresis Detection ゲル電気泳動シュリーレン光学系

项目摘要

Polyacrylamide gel electrophoresis is suitable for analysis and separation of proteins and nucleic acids, and gives results with high sensitivity and high resolution. Beautiful patterns of separation is, however, only possible to be visualized after staining of the gel. The aim of the present study was to establish a method by which electrophoretic patterns in polyacrylamide gel electrophoresis can be visualized in a real-time mode by the use of schlieren optics.As the outcome of our effort, bands of proteins of nucleic acids can now be detected by the optics with sensitivity comparable to the routine conventional staining procedures. Use of an image analyzer together with the optics makes it possible to store and analyze the data obtained by a personal computer. The optics can also be utilized for localization of a sample band without staining and sampling the corresponding part for sample collection. The optics can also be effectively used for visualization of separation of protein bands in a concentrated crosslinker-free polyacrylamide solution. Since such linear polymer solutions are now finding practical value as media for capillary electrophoreses, the schlieren optics developed in the present study should provide basic data which may be efficient in designing such media for capillary electrophoresis.

聚丙烯酰胺凝胶电泳适用于蛋白质和核酸的分析和分离，并具有高灵敏度和高分辨率的结果。然而，仅在凝胶染色后才能看到美丽的分离模式。本研究的目的是建立一种方法，该方法通过使用Schlieren光学功能，可以在实时模式下可视化聚丙烯酰胺凝胶电泳中的电泳模式。由于我们努力的结果现在可以通过与常规常规染色程序相比敏感的核酸蛋白质来检测到核酸的蛋白质。使用图像分析仪与光学器件一起可以存储和分析个人计算机获得的数据。光学器件也可以用于定位样品带，而无需染色和采样样品收集的相应零件。光学元件也可以有效地用于可视化浓缩的无交叉链接聚丙烯酰胺溶液中蛋白质带的分离。由于现在这种线性聚合物溶液正在发现作为毛细管电泳培养基的实用价值，因此本研究中开发的Schlieren光学产品应提供基本数据，这些数据可能有效地为毛细管电泳设计这种介质。