Development of a Schlieren Optics for Real-Time Visualization of Polyacrylamide Gel Electrophoresis

开发用于聚丙烯酰胺凝胶电泳实时可视化的纹影光学器件

• 批准号: 63880019
• 负责人: TAKAGI Toshio
• 金额: $ 6.34万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Developmental Scientific Research (B).
• 财政年份: 1988
• 资助国家: 日本
• 起止时间: 1988 至 1990
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-63880019/
• 关键词: Polyacrylamide; Gel Electrophoresis; Detection; ゲル電気泳動; シュリーレン光学系

Polyacrylamide gel electrophoresis is suitable for analysis and separation of proteins and nucleic acids, and gives results with high sensitivity and high resolution. Beautiful patterns of separation is, however, only possible to be visualized after staining of the gel. The aim of the present study was to establish a method by which electrophoretic patterns in polyacrylamide gel electrophoresis can be visualized in a real-time mode by the use of schlieren optics.As the outcome of our effort, bands of proteins of nucleic acids can now be detected by the optics with sensitivity comparable to the routine conventional staining procedures. Use of an image analyzer together with the optics makes it possible to store and analyze the data obtained by a personal computer. The optics can also be utilized for localization of a sample band without staining and sampling the corresponding part for sample collection. The optics can also be effectively used for visualization of separation of protein bands in a concentrated crosslinker-free polyacrylamide solution. Since such linear polymer solutions are now finding practical value as media for capillary electrophoreses, the schlieren optics developed in the present study should provide basic data which may be efficient in designing such media for capillary electrophoresis.

聚丙烯酰胺凝胶电泳适用于蛋白质、核酸的分析分离，结果灵敏度高、分辨率高。然而，只有在凝胶染色后才能看到美丽的分离图案。本研究的目的是建立一种方法，通过使用纹影光学，聚丙烯酰胺凝胶电泳中的电泳模式可以实时可视化。作为我们努力的成果，核酸的蛋白质条带现在可以通过光学器件检测，其灵敏度与常规染色程序相当。将图像分析仪与光学器件一起使用可以存储和分析个人计算机获得的数据。该光学器件还可用于定位样品带，而无需对样品收集的相应部分进行染色和取样。该光学器件还可以有效地用于可视化浓缩的无交联剂聚丙烯酰胺溶液中蛋白质条带的分离。由于此类线性聚合物溶液现已发现作为毛细管电泳介质的实用价值，因此本研究中开发的纹影光学器件应提供可有效设计用于毛细管电泳的此类介质的基本数据。

项目成果

Toshio Takagi,Hidehiro Kubota and Seizo Ohishi: "Application of Schlieren Optics for Realーtime Monitoring of Protein Electrophoresis in Crosslinkーfree Linear Polyacrylamide Solution" Electrophoresis.

Toshio Takagi、Hidehiro Kubota 和 Seizo Ohishi：“应用纹影光学实时监测无交联线性聚丙烯酰胺溶液中的蛋白质电泳”电泳。

高木 俊夫 他5名: "ポリアクリルアミドゲル電気泳動" 広川書店, 172 (1990)

高木敏夫等 5 人：《聚丙烯酰胺凝胶电泳》广川书店，172（1990）

T.Takagi;P.F.Rao;Y.Sato: Proceedings of the Sixth International Electrophoresis Society(Electrophoresis '88). 344-354 (1988)

T.Takagi；P.F.Rao；Y.Sato：第六届国际电泳学会论文集（Electrophores 88）。

Toshio Takagi, Hidehiro Kubota, Seizou Ohishi: "Application of Schlieren Optics for Real-time Monitoring of Protein Electrophoresis in Crosslinker-free Linear Polyacrylamide Solution" Electrophoresis.

Toshio Takagi、Hidehiro Kubota、Seizou Ohishi：“应用纹影光学实时监测无交联剂线性聚丙烯酰胺溶液中的蛋白质电泳”电泳。

高木 俊夫他5名: "ポリアクリルアミドゲル電気泳動" 広川書店, 172 (1990)

高木敏夫等 5 人：《聚丙烯酰胺凝胶电泳》广川书店，172（1990）