Inhibition of tumor neovascularizatuon in the experimental brain tumors by hydrocortisone.

氢化可的松抑制实验性脑肿瘤中的肿瘤新生血管化。

• 批准号: 63570692
• 负责人: SUZUKI Yasuo
• 金额: $ 1.22万
• 依托单位: Kawasaki Medical School
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1988
• 资助国家: 日本
• 起止时间: 1988 至 1989
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-63570692/
• 关键词: Microvascular architecture; Corrosion cast; Experimental brain tumor; Bromodeoxyuridine; Anti-angiogenesis; 免疫組織化学; 走査電顕

Tumor microcirculation is of crucial importance not only for the metabolism and behavior of tumor tissue but also for tumor therapy. Scanning electron microscopy of vascular corrosion casts and immuno- cytochemical study was undertaken to demonstrate the inhibition of the tumor neovasucularization by hydrocortisone in ethyinitrosourea induced brain tumors. Although a reduction in tumor developmental rate was observed in rats treated with hydrocotisone, microvascular architecture and bromodeoxyuridine(BrdU) labeled endothelial cells did not varied with or without hydrocortisone. In tumors less than 2mm in diameter no tumor vessels and BrdU labeled endothelial cells were observed, indicating an avascular phase of tumor growth. On the other hand, in tumors more than 2 mm in diameter, various vascular phases were observed. In particular, the peripheral zone of the tumors consisted of dilated and tortuous vessels and rich BrdU labeled endothelial cells which may be characteristic of angiogenesis.

肿瘤微循环不仅对肿瘤组织的代谢和行为至关重要，而且对肿瘤疗法至关重要。进行了扫描电子显微镜，对血管腐蚀铸件和免疫细胞化学研究进行了扫描，以证明氢化可的松在乙硝基乙酸酯诱导的脑肿瘤中抑制肿瘤新可核酸化。尽管在用氢固醇，微血管结构和标记为内皮细胞标记的溴脱氧尿苷（BRDU）处理的大鼠中观察到肿瘤发育率的降低并未随着氢化可的松的含量而变化。在直径小于2mm的肿瘤中，没有肿瘤血管和BRDU标记的内皮细胞，表明肿瘤生长的血管相。另一方面，在直径超过2 mm的肿瘤中，观察到各种血管相。特别是，肿瘤的周围区域由扩张和曲折的血管和丰富的BRDU标记为内皮细胞，这可能是血管生成的特征。

项目成果

菊岡政久: 川崎医学会誌. 15. (1988)

菊冈正久：川崎医学会杂志 15。（1988）。

菊岡政久: "Ethylnitrosourea誘発ラット実験脳腫瘍の微小血管構築に関する研究-血管鋳型走査電顕法による検討-" 川崎医学会誌. 15. 62-71 (1989)

Masahisa Kikuoka：“乙基亚硝基脲诱导的实验性大鼠脑肿瘤的微血管构建研究 - 使用血管模具扫描电子显微镜检查 -”川崎医学会杂志 15. 62-71 (1989)。

菊岡政久: "ラット脳における微小血管構築の検討-血管鋳型走査電顕法による解析-" 川崎医学会誌. 14. 539-551 (1988)

Masahisa Kikuoka：“大鼠大脑微血管结构的检查 - 使用血管模具扫描电子显微镜进行分析”川崎医学会杂志 14. 539-551 (1988)。

菊岡政久: 川崎医学会誌. 14. (1989)

菊冈正久：川崎医学会杂志 14。（1989）。

菊岡政久: "Ethylnitrosourea誘発ラット実験脳腫瘍の微小血管構築に関する研究ー血管鋳型走査電顕法による検討ー" 川崎医学会誌. 15. 62-71 (1989)

Masahisa Kikuoka：“乙基亚硝基脲诱导的实验性大鼠脑肿瘤中微血管的构建研究 - 使用血管模具扫描电子显微镜检查”川崎医学会杂志 15. 62-71 (1989)。