Studies for Genesis and treatment of endometriosis-associated infertility: In connection with an ovum capture inhibitor (OCI).

子宫内膜异位症相关不孕症的起源和治疗研究：与卵子捕获抑制剂（OCI）有关。

• 批准号: 62570761
• 负责人: SUGINAMI Hiroshi
• 金额: $ 1.34万
• 依托单位: Ehime University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1987
• 资助国家: 日本
• 起止时间: 1987 至 1988
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-62570761/
• 关键词: Endometriosis; Ovum capture inhibitor; Peritoneal fluid; Infertility; in vitro卵捕獲 実験; 走査電顕

The present study was conducted to demonstrate in endometriosis peritoneal fluid (PF) an ovum capture inhibitor (OCI) as a causative factor for endometriosis-associated infertility and, if any, to investigate its action mechanism. A mouse oocytecumulus complex was repeatedly applied on a golden hamster oviductal fimbria immersed in endometriosis or non-endometriosis PF. The period from the initiation of the experiment until the termination of fimbrial activity of ovum capture, ovum capture didappearance time (OCDT), was significantly reduced with native, cell-free and macromolecular fractions of endometriosis PF. A prolongation in OCDT was noted with PF specimens taken from endometriosis women under treatment with Danazol, an agent known to increase fecundity in endometriosis. Scanning electron microscopy disclosed membranous deposits (OCI-related membrane) on the fimvria exposed to endometriosis PF, by which ciliated fimbrial epithelial cells were completely concealed. A retrograde tubal flush for the oviduct exposed to endometriosis PF first ballooned and then removed the OCI-related membrane. Flushed oviducts resumed fimbrial activity of ovum capture. Laparoscopy, when performed on endometriosis women whose peritoneal cavity was filled with saline, demonstrated a balloon formation of possible OCI-related membranes on the fimbria under chromotubation. The OCI, reducing fimbrial activity of ovum capture, is abundantly present in endometriosis PF. It is a water-soluble macromolecule. Danazol treatment mught decrease OCI acgtivity. The OCI reduces fimbrial activity of ovum capture through preventing the cumulus-fimbrial interaction by the OCI-related membrane deposited on the oviductal fimbria.

本研究是为了在子宫内膜异位症（PF）中证明卵子捕获抑制剂（OCI），作为子宫内膜异位相关的不育的病因，并研究其作用机制。浸入子宫内膜异位症或非内膜异位症PF中的金仓鼠卵巢纤维膜上反复应用小鼠卵母细胞复合物。从实验启动到卵子捕获的纤维化活性，卵形捕获时间（OCDT）的终止的时期，用天然，无细胞和大分子分子的子宫内膜异位症PF显着降低。在OCDT中延长了OCDT的延长，其中PF标本是从子宫内膜异位症妇女接受治疗的Danazol的PF样本，Danazol是一种已知增加子宫内膜异位症的毒剂。扫描电子显微镜在暴露于子宫内膜异位症PF的Fimvria上揭示了膜沉积物（与OCI相关的膜），通过该膜沉积物完全隐藏了纤毛的纤毛上皮细胞。暴露于子宫内膜异位症PF的输卵管的逆行输管冲洗首先散发出，然后去除与OCI相关的膜。冲洗卵形恢复了卵子捕获的纤维化活性。腹腔镜检查在子宫内膜异位症的妇女上进行腹膜腔填充盐水时，表现出在染色体下的纤毛上可能与OCI相关的膜形成的球囊。 OCI在子宫内膜异位症PF中大量存在卵子捕获的纤维化活性。它是水溶性的大分子。 Danazol治疗MUGHT降低了OCI的影响。 OCI通过防止沉积在输卵管纤维膜上的OCI相关膜的脑相互作用来降低卵子捕获的纤维化活性。

项目成果

Suginami・H,;Yano,K・: Fertil Steril. 50. 648-653 (1988)

杉并·H、矢野、K·：《生育力》50. 648-653 (1988)

YANO, KOHJI: "Aduances in Fertility and Sterility Series, Voulumes 5 Eudo metriosis" The Parthenon Publishing Group, 236 (1987)

YANO, KOHJI：“生育和不育系列的进展，第 5 卷 Eudo metriosis”帕台农神庙出版集团，236（1987）

Suginami H; Yano K.: "An ovum capture inhibitor (OCI) in endometriosis peritoneal fluid: an OCI-relatod membrane responsible for fimbrial failure of ovum capture." Fertility and Sterility. 50. 648-653 (1988)

杉并H；

Yano K; Suginami H; Matsuura S.: An ovum capture inhibitor in endometriosis peritoneal fluid.Parthenon Publishing, Lancaster, UK., 41-47 (1987)

矢野K;

矢野浩史、杉並洋、松浦俊平、植田規史: エンドメトリオージス研究会誌. 9. 141-143 (1988)

Hiroshi Yano、Hiroshi Suginami、Shunpei Matsuura、Norifumi Ueda：子宫内膜异位症研究组杂志。9. 141-143 (1988)。