Growth of the Acetabulum - Role of the Secondary Ossification Centers

髋臼的生长 - 二级骨化中心的作用

基本信息

批准号：
62570692
负责人：
AZUMA Hirohiko
金额：
$ 1.34万
依托单位：
Saitama Medical School
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1987
资助国家：
日本
起止时间：
1987 至 1988
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-62570692/
关键词：
Acetabulum Secondary Ossification Center Growth Cartilage 発育

项目摘要

Development of the secondary ossification centers of the acetabulum was studied using infantile rats and rabbits. Results revealed that ossification developed in rats and rabbits at 2-3 weeks and 3-4 weeks after birth, respectively, and was completed at 10-12 weeks. It was clarified that of these secondary ossification centers, the os acegabuli which belonged to the pubic bone underwent the maximum size.Stimulation by weak direct electric current was performed in the os acetabuli using 4-week-old male rebbits for 3 and 4 weeks. As the means of embedding the electrode, the cathode was inserted from the medial side of the pubic bone toward the os acetabuli, and the tip of the cathode was designed to extend to the os acetabuli. The anode was fixed in the gluteal muscle, and stimulation apparatus was placed subcutaneously on the abdomen.With regard to the growth of the acetabulum, difference at the 5% level of significance in the depth was noted in the 3-week-electric current group compared with the control group. No significant difference was noted in the 4-week-electric current group. No significant difference in diameter was noted in both the 3- and 4- week-electric current groups.Amplification of the growing cartilage, especially proliferation of the proliferative cell cayer was histologically noted. However, growth of this proliferation of cartilage cells was noted only in the pari-electrode region, and only irregularity of the cartilage zone was obserbved.The above-mentioned experimental results, suggest the possibility of influence by weak direct electric current to the process of ossification in the growing cartilage, although a definite tendency could not be determined.

使用幼年大鼠和兔子研究了髋臼次级骨化中心的发育。结果显示，大鼠和兔子分别在出生后2-3周和3-4周开始骨化，并在10-12周时完成。结果表明，在这些次生骨化中心中，属于耻骨的髋臼骨尺寸最大。使用4周龄雄性兔子对髋臼骨进行弱直流电刺激3周和4周。作为埋置电极的方式，阴极从耻骨内侧向髋臼口插入，并且阴极尖端设计为延伸至髋臼口。将阳极固定在臀肌上，将刺激装置皮下放置在腹部。关于髋臼的生长，在3周电流组中观察到深度的5％显着性差异与对照组相比。 4 周电流组没有发现显着差异。在3周和4周电流组中没有观察到直径的显着差异。在组织学上注意到生长的软骨的扩增，特别是增殖细胞膜的增殖。然而，这种软骨细胞增殖的生长仅在对电极区域被观察到，且仅观察到软骨区域的不规则性。上述实验结果表明弱直流电对软骨细胞增殖过程的影响的可能性。生长中的软骨发生骨化，尽管无法确定明确的趋势。