Nuclear DNA analysis of genetics of cancer and dysplasia complicating ulcerative colitis

癌症和溃疡性结肠炎并发不典型增生的遗传学核 DNA 分析

基本信息

批准号：
62570593
负责人：
NAGAI Hideo
金额：
$ 0.9万
依托单位：
University of Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1987
资助国家：
日本
起止时间：
1987 至 1988
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-62570593/
关键词：
Microspectrophotometry DNA Ulcerative colitis Dysplasia Aneuploid Polyploid Surueillance colonoscopy 細胞核DNA量癌化

项目摘要

1)Assessment of optimum thickness of the sections : This study assessed the optimum thickness of the paraffin embedded sections to determine the true DNA content with a cytophofometric technique in 35 colonic samples from 23 patients with ulcerative colitis and 10 non-colitic patients with colorectal cancer. The 4 ,7 ,10 sections were cut from each samples. The cellular DNA values of these sections were compared with smears from cells detached from the same paraffin embedded tissue. The modal DNA values of the four methods were similar. On the contrary, the frequency of the cells with DNA content higher than 6C value (polyploid cells) did not coincide. The frequency of false negative cases which had polyploid cells in smears but hadn't in sections was 8.6, 0, 5.7 in 4, 7, 10 respective. The frequency of false positive cases was 14.3, 20, 25.7, but 6.3, 6.7, 18.8 in the inactive specimens. Therefore 7 section showed higher sensitivity and specificity. We suqqest that micro spectrophotom … More etric DNA content in ulcerative colitis is better analized with 7 section.2)Application for cancer and dysplasia diagnpsis : The DNA content was measured by microspectrophotometry from 100 specimens in 60 patients with ulcerative colitis including six complicating carcinoma. In dysplastic 23 (77%) of 30 samples showed aneuploidy or polyploidy, whereas in non-dysplastic tissues 50 (94%) of 53 samples were diploid, the difference being statistically significant. Polyploidy was often observed in the non-dysplastic mucosa of the patients complicating cancer or dysplasia. In non-dysplastic patients all sample with inflammation showed diploidy, however 10% of samples without inflammation showed polyploidy. A good correlation was found between the incidence of polyploid cells and the grade of dyspasia. From this study it was assumed that microspectrophotometric measurement of DNA content is useful in the assessment and siagnosis of dysplasia in ulcerative colitis and can be available in the screening of high-risk patients. Less

1）评估最佳部分：在35例溃疡性结肠炎患者的35例结肠样品中，用细胞量计和10个非层状患者进行了4、7、7、10个样品中的35例无体样品中的35例结肠样品中，在35例结肠样品中，从每个样品中切出了4、7、7、7 。 . The YPLOID CELLS in Smetars But Hadn't in Secrets Was 8.6, 0, 5.7 in 4, 7, 10 Respective. The Frequency of False Casses Wass 14.3, 20, 25.7, But 6.3, 6.7, 18.8 in The Inactive Specimens. Nsitivity和特异性。在30个样品中，有23个样本中的23（77％）在非塑性组织中表现出动脉粥样硬化或多倍性，而53个样品中的50个样本是二倍体的非塑性粘膜炎症的所有样本显示，炎症的样品中有10％的样本显示了肿瘤的肿瘤症状。