Nuclear DNA analysis of genetics of cancer and dysplasia complicating ulcerative colitis

癌症和溃疡性结肠炎并发不典型增生的遗传学核 DNA 分析

基本信息

批准号：
62570593
负责人：
NAGAI Hideo
金额：
$ 0.9万
依托单位：
University of Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1987
资助国家：
日本
起止时间：
1987 至 1988
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-62570593/
关键词：
Microspectrophotometry DNA Ulcerative colitis Dysplasia Aneuploid Polyploid Surueillance colonoscopy 細胞核DNA量癌化

项目摘要

1)Assessment of optimum thickness of the sections : This study assessed the optimum thickness of the paraffin embedded sections to determine the true DNA content with a cytophofometric technique in 35 colonic samples from 23 patients with ulcerative colitis and 10 non-colitic patients with colorectal cancer. The 4 ,7 ,10 sections were cut from each samples. The cellular DNA values of these sections were compared with smears from cells detached from the same paraffin embedded tissue. The modal DNA values of the four methods were similar. On the contrary, the frequency of the cells with DNA content higher than 6C value (polyploid cells) did not coincide. The frequency of false negative cases which had polyploid cells in smears but hadn't in sections was 8.6, 0, 5.7 in 4, 7, 10 respective. The frequency of false positive cases was 14.3, 20, 25.7, but 6.3, 6.7, 18.8 in the inactive specimens. Therefore 7 section showed higher sensitivity and specificity. We suqqest that micro spectrophotom … More etric DNA content in ulcerative colitis is better analized with 7 section.2)Application for cancer and dysplasia diagnpsis : The DNA content was measured by microspectrophotometry from 100 specimens in 60 patients with ulcerative colitis including six complicating carcinoma. In dysplastic 23 (77%) of 30 samples showed aneuploidy or polyploidy, whereas in non-dysplastic tissues 50 (94%) of 53 samples were diploid, the difference being statistically significant. Polyploidy was often observed in the non-dysplastic mucosa of the patients complicating cancer or dysplasia. In non-dysplastic patients all sample with inflammation showed diploidy, however 10% of samples without inflammation showed polyploidy. A good correlation was found between the incidence of polyploid cells and the grade of dyspasia. From this study it was assumed that microspectrophotometric measurement of DNA content is useful in the assessment and siagnosis of dysplasia in ulcerative colitis and can be available in the screening of high-risk patients. Less

1）评估各切片的最佳厚度：本研究评估了Popaffin嵌入切片的最佳厚度，以确定来自23例溃疡性结肠炎患者和10名非癌症患者结肠癌患者的35例结肠样品中的细胞体样品中的真实DNA含量。从每个样品中切出4,7,10个部分。将这些切片的细胞DNA值与从同一石蜡嵌入组织的细胞的涂片进行了比较。四种方法的模态DNA值相似。相反，DNA含量高于6C值（多倍体细胞）的细胞频率不一致。涂片中有多倍体细胞但没有在部分中没有多倍细胞的假阴性病例的频率为4.6、0、5.7，4、7、10相对。假阳性病例的频率为14.3、20、25.7，但在非活性标本中为6.3、6.7、18.8。因此，7个切片显示出更高的灵敏度和特异性。我们将微分分光光聚光体...溃疡性结肠炎中更多的Etric DNA含量可以通过7个切片进行更好的分析。2）癌症和发育异常诊断的应用：DNA含量是通过从100个标本中的100个标本中测量的DNA含量，包括60例溃疡性结肠炎患者，包括6例复杂的癌症。在发育不良的23（77％）中，有30个样品显示出非整倍性或多倍体，而在非塑性组织中，50（94％）在53个样品中是二倍体，差异具有统计学意义。在患者或发育不良的患者的非塑性粘膜中经常观察到多倍体。在非塑性患者中，所有患有炎症样本的样本显示了二倍体，但是有10％的没有炎症的样本显示了多倍体。在多倍体细胞的事件和冬季等级之间发现了良好的相关性。从这项研究中可以假定，DNA含量的微光谱测量值可用于溃疡性结肠炎中发育不良的评估和诊断，并且可以在筛查高危患者的筛查中提供。较少的