Anatomical visualization of adenosinergic neurons in the rat brain

大鼠大脑中腺苷能神经元的解剖可视化

基本信息

批准号：
62570026
负责人：
SENBA Emiko M.D. Ph.D.
金额：
$ 1.54万
依托单位：
Osaka University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1987
资助国家：
日本
起止时间：
1987 至 1988
项目状态：
已结题

项目摘要

Adenosine has been implicated as a modulator of neural transmission, in addition to its ubiquitous involvement in intermediary metabolism. In both central and peripheral tissues, the localization of adenosine receptors and associated adenylate cyclases by anatomical and pharmacological means has provided some clues about sites of action of this substance. An accumulation of electrophysiological studies has shown that adenosine is an inhibitory neurotransmitter in the central nervous system.Our interest has centered on the identification of markers which would allow the anatomical visualization of adenosinergic neurons. We chose to investigate the possibility that the enzymes responsible for synthesis or degradation of adenosine may be markers for neurons in which adenosine serves a neuromodulatory function. Specific antisera against adenosine, S-adenosyl homocysteinase (SAH)(synthesizing enzyme) and adenosine deaminase (ADA)(degrading enzyme) were raised in rabbits. To date, a relative … More ly restricted distribution of neurons was investigated. The latter neurons showed rather ubiquitous distribution in the brain compared to ADA containing neurons.For further anatomical analysis, we chose retino-collicular pathway as a model, because abundant adenosine receptors are identified in the superficial superior colliculus and numerous ADA-containing neurons are distributed in the same layer (E. Senba et al, 1987; J.J.Miguell et a., 1989). These ADA immunoreactive neurons were shown to project to the postero-lateral nucleus of thalamus (J.J.Miguell et al,1988). In the retina, adenosine-IR and/or SAH-IR neurons were located in the ganglion cell layer and these neurons were shown to be ganglion cells (Senba et al, in preparation). ADA-containing neurons were also observed in the same layer, but these neurons were demonstrated to be displaced amacrine cells (E.Saenba et al.,1986) Immunoelectron microscopy combined with degeneration method revealed that dendrites of these ADA contianing neurons receive direct axonal inputs from the retina (J.J.Miguell, in preparation). Less

腺苷除了普遍参与中枢和外周组织的中间代谢外，还被认为是神经传递的调节剂，通过解剖学和药理学手段对腺苷受体和相关腺苷酸环化酶的定位提供了有关作用位点的一些线索。大量的电生理学研究表明，腺苷是中枢神经系统中的一种抑制性神经递质。我们的兴趣集中在鉴定上。我们选择研究负责腺苷合成或降解的酶可能是腺苷发挥神经调节功能的特异性抗血清的可能性。迄今为止，SAH（合成酶）和腺苷脱氨酶（ADA）（降解酶）是近亲。 …与后来含有 ADA 的神经元相比，研究了更受限制的神经元分布。为了进一步的解剖学分析，我们选择视网膜-丘脑通路作为模型，因为在浅表中发现了丰富的腺苷受体。上丘和大量含有 ADA 的神经元分布在同一层（E. Senba 等，1987；J.J. Miguell 等，1989）。 ADA 免疫反应性神经元被证明投射到丘脑的后外侧核（J.J.Miguell 等人，1988）。在视网膜中，腺苷-IR 和/或 SAH-IR 神经元位于神经节细胞层，并且这些神经元被显示。是神经节细胞（Senba等人，在准备中）也在同一层中观察到含有ADA的神经元，但这些神经元被证明是移位的无长突细胞。 (E.Saenba et al.,1986) 免疫电子显微镜结合变性方法揭示了这些含有 ADA 的神经元的树突接收来自视网膜的直接轴突输入（J.J.Miguell，准备中）。