Examination of Signals for Initiation of DNA Synthesis on Single Strand, and their Consensus Nucleotide Sequences

单链 DNA 合成起始信号及其共有核苷酸序列的检查

基本信息

批准号：
62560083
负责人：
SAKAI Hiroshi
金额：
$ 1.34万
依托单位：
Kyoto University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1987
资助国家：
日本
起止时间：
1987 至 1988
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-62560083/
关键词：
recombinant DNA origin of DNA replication signals for single-strand DNA initiation ssi signal plasmid priming 繊維状ファージベクター

项目摘要

Genomes of plasmids pACYC184, RSF1010 and pSY343 carries one or two ssi signals. They are diverse in their size and structure. Signals which are operated by the same enzyme system are often different in their structure. However, with the ssi signals thus far identified, two structural features are found in common. First, the ssi signal-determining sequences contain nucleotide sequences homologous to a concensus sequence 5^1-GAAGCGG-3^1 found in the E. coli N^1 protein recognition sites. Second, potential stem-loop structures are contained in the ssi regions. Although the common structural features seem to play important roles, their mode of contribution to ssi function is not revealed yet.The ssi signals identified in the present studies are diverse in their mode of function and biological significance, and can be grouped into three classes. The first class; the ssi signals which are located in the oriV region, and direct the synthesis of primer RNA for DNA chain elongation of the lead … More ing strand (ssiA and ssiB of RSF1010, and ssiA of pSY343). The second class; the ssi signals which are located separately from the region directing the leading strand initiation, and seem to direct the lagging strand DNA synthesis (ssi of pACYC184). The third class; the ssi signals which are located far apart from the region directing the leading strand initiation, and might direct the DNA synthesis during conjugative transmission of plasmids, or might play some roles for efficient progress of chain elongation furing vegetative DNA replication (ssiB of pSY343). Because the diverse biological properties of the ssi signals reflect the evolutional process of replicons, ssi signals will be a powerful criterion of searching for origins of DNA-replicating machinery.The function of DNA-replicating machinery is ultimately attributed to that of ssi signals which is directly involved in providing RNA primers for DNA chain elongation. For the future, many other ssi signals should be examined to understand the whole structural and functional aspect of ssi signals, and structural elements determining the specific biological properties should be revealed. Studies on this line should be advanced for making it possible to design artificial DNA-replicating machinery suiting with specific purposes. Less

质粒 pACYC184、RSF1010 和 pSY343 的基因组携带一或两个 ssi 信号，它们的大小和结构不同。由相同酶系统操作的信号通常在结构上有所不同。发现两个共同的结构特征：首先，ssi信号决定序列包含与共有序列同源的核苷酸序列。在大肠杆菌 N^1 蛋白质识别位点中发现了 5^1-GAAGCGG-3^1 其次，潜在的茎环结构包含在 ssi 区域中，尽管共同的结构特征似乎发挥着重要作用，但它们的模式。目前研究中发现的 ssi 信号在功能模式和生物学意义方面各不相同，可分为三类：第一类；位于 oriV 区域的 ssi 信号。 ,并指导前导链 DNA 链延伸的引物 RNA 的合成（RSF1010 的 ssiA 和 ssiB，以及 pSY343 的 ssiA）；第二类 ssi 信号与指导前导链起始的区域分开。，并且似乎指导滞后链DNA合成（pACYC184的ssi）第三类ssi信号，其位置远离该区域；指导前导链的起始，并可能在质粒接合传递过程中指导 DNA 合成，或者可能在 DNA 营养复制（pSY343 的 ssiB）的链延长过程中发挥一些作用，因为 ssi 信号的不同生物学特性反映了其高效性。在复制子的进化过程中，ssi信号将成为寻找DNA复制机制起源的有力标准。DNA复制机制的功能最终要归功于ssi信号的功能其直接参与为 DNA 链延伸提供 RNA 引物。未来，应检查许多其他 ssi 信号，以了解 ssi 信号的整体结构和功能，并揭示其特定生物学特性的结构决定元件。这条生产线应该先进，以便能够设计出适合特定用途的人工 DNA 复制机器。