Analysis of immunological mechanisms for antibody production to hepatitis B surface antigen.

乙型肝炎表面抗原抗体产生的免疫机制分析。

基本信息

批准号：
60570317
负责人：
KAKUMU Shinichi
金额：
$ 1.15万
依托单位：
Nagoya University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1985
资助国家：
日本
起止时间：
1985 至 1986
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-60570317/
关键词：
anti-HBs production human peripheral blood mononuclear cells purified HBsAg anti-idiotypic antibody interleukin 2 pokeweed mitogen ポークウィードマイトロジェン

项目摘要

1. Enhancement of antibody production to hepatitis B surface antigen by anti-idiotypic antibody. Studies were undertaken to determine whether anti-idiotypic antibody against antibody to hepatitis B surface antigen (anti-HBS) could modulate in vitro anti-HBS production by human peripheral blood mononuclear cells stimulated with pokeweed mitogen. Peripheral blood mononuclear cells from patients positive for serum anti-HBs produced significantly increased amounts of anti-HBs by the addition of F <(ab')_2> fragments of anti-anti-HBs as well as purified HBsAg when compared to those in cultures with pokeweed mitogen alone, indicating that anti-idiotypic antibody modulates the immune response to HBsAg.2. In vitro synthesis of antibody to hepatitis B virus antigen by circulating lymphocytes from chronic HBsAg carriers and patients with acute hepatitis B. The synthesis of IgG, anti-HBs and anti-HBc was measured in pokeweed mitogen-stimulated cultures of peripheral blood mononuclear cells from chronic HBsAg carriers, patients with acute hepatitis B (HAB) and subjects with anti-HBs in serum (controls). Coculture experiments indicated that decreased B cell function and increased suppressor T cell function specific for HBsAg contributed to the lack of anti-HBs in serum of chronic HBsAg carriers and AHB patients during the recovery phase.3. Enhancement of antibody production to hepatitis B surface antigen by interleukin 2. We studied to assess whether induction of anti-HBs production by cultured lymphocytes which had been conducted with pokeweed mitogen could be replaced by interleukin 2 (IL2). In cultures stimulated with purified HBsAg and IL2, the comparable levels of anti-HBs production to those obtained in cultures stimulated with pokeweed mitogen alone occured when peripheral blood mononuclear cells from patients positive for serum anti-HBs and recipients of HBsAg vaccine were used as effector cells. However, IL2 alone did not induce anti-HBs production.

1。通过抗IDiotypic抗体增强对乙型肝炎表面抗原的抗体产生。进行研究以确定抗丙型肝炎表面抗原（抗HBS）抗体的抗二动抗体是否可以通过人外周血单核细胞在用pokeweed sitiT刺激的人类外周血单核细胞中调节体外抗HBS的产生。与培养物相比，通过添加f <（ab'）_ 2>抗HBS的F <（AB'）_ 2>片段，与抗抗HBS的f <（ab'）_ 2>片段相比，与培养物相比，血清抗HBS阳性的患者外周血单核细胞显着增加了抗HBS的量。仅用pokeweed有丝分裂原而表明抗IDiotypic抗体会调节对HBSAG的免疫反应2。通过慢性HBSAG载体和急性丙型肝炎的患者循环淋巴细胞的循环淋巴细胞和急性丙型肝炎B的循环淋巴细胞的体外合成。慢性HBSAG携带者，急性肝炎B（HAB）患者和血清中抗HBS的受试者（对照）。共培养实验表明，在恢复阶段，慢性HBSAG携带者和AHB患者的血清中缺乏抗HB的B细胞功能和抑制T细胞功能的降低和抑制剂T细胞功能增加。3。通过白介素2增强对丙型肝炎表面抗原的抗体产生。我们研究以评估是否可以通过介导的培养的淋巴细胞诱导抗HBS产生，这些淋巴细胞用pokeweed有丝分裂原进行的培养淋巴细胞可以用白介素2取代（IL2）。在用纯化的HBSAG和IL2刺激的培养物中，仅在外周血单核细胞中，抗Hbs的抗HBS产生水平可比的水平与在血清抗HBS阳性的患者和HBSAG疫苗的受益者阳性的患者中单独刺激的培养物中获得的可比水平。细胞。但是，仅IL2并未诱导抗HBS产生。