Pathology of cells induced by paramyxovirus infection

副粘病毒感染诱导的细胞病理学

• 批准号: 60440036
• 负责人: OKADA Y.
• 金额: $ 12.1万
• 依托单位: Institute for Molecular and Cellular Biology, Osaka University (1985-1986, 1988)Tokyo Institute of Technology (1987)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (A)
• 财政年份: 1985
• 资助国家: 日本
• 起止时间: 1985 至 1988
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-60440036/
• 关键词: cDNA of F and HANA; fusion; naked lipid bilayer; cDNA; 限定分解; 毒性; NDV; cDNA注入; 細胞融合; 量子井戸構造; 光スイッチ; 半導体レーザ; モノリシック光集積回路; 多次元量子井戸構造; 金属; 絶縁体超格子構造; FcDNA; HNcDNA; C蛋白質; Fタンパク質; HANAタンパク質; ペプチドの人工合成; HNタンパク質; cDNA of F and HANA; fusion; naked lipid bilayer; cDNA; 限定分解; 毒性; NDV; cDNA注入; 細胞融合; 量子井戸構造; 光スイッチ; 半導体レーザ; モノリシック光集積回路; 多次元量子井戸構造; 金属; 絶縁体超格子構造; FcDNA; HNcDNA; C蛋白質; Fタンパク質; HANAタンパク質; ペプチドの人工合成; HNタンパク質

1. Mechanism of cell fusion by HVJ: On the basis of our experimental data, a hypothesis of the mechanism has been presented. After adsorption of HVJ onto cells, Fl N-terminal fusogenic domain inserted into cell membranes udergo conforma-tional change at 37゜C. This results in the folding in cholesterol molecule and the perturbation of the lipid bilayer, followed by marked damage of the cell mem-brane. This damage in turn induces disarragement of the cytoskeletal system, caused by the sudden introduction of Ca ions into the cytoplasm, and allows the membrane proteins to become freely mobile. The naked lipid bilayers formed by the clustering of IMP(Intramembrane particle) can then come into direct contact without interference from IMPs. In thee areas of attachment the cell membranes promptly fuse. Thus, the fundamental mechanism of cell fusion by HVJ seems to be similar to the fusion mechanism by PEG, electroporation and microsurgery of cell membranes.2. This presentation is supported by … More the finding as follows: It has been demon-strated that aged HVJ virions easily fuse together by collision, but young ones immediately after the budding from infected cells show resistancy to this kind of fusion. It has been know that IMP are aggregated together in the envelope of aged virions, because of the disruption of the connection with M protein.3. Simple liposomes can fuse with cells fusing together: It has been reported that liposomes containing diphtheria toxin fragment A show the activity of selective killing of HIV infected cells fusing with B-lymphocytes. It is possible to condiser that naked lipid bilayers are exposed on these cell surfaces and the liposomes become able to fuse with the areas.4. cDNA of F and HANA glycoproteins have been isolated from HVJ or NDV infected cells. These cDNA plasmids containing SV40 ori showed high expressions when intro-duced in COS cells. Now expression of plasmids containing these cDNA and actin promoter are being observed in LLCMK2, CV1 and L cells. This project has not yet completed. Less

1。通过HVJ的细胞融合机理：根据我们的实验数据，已经提出了该机制的假设。将HVJ添加到细胞上后，将FL N末端融合域插入了细胞膜Udergo构造的37°C下变化。这导致胆固醇分子的折叠和脂质双层的扰动，然后明显的细胞内膜损伤。反过来，这种损害会引起细胞骨架系统的混乱，这是由于突然将CA离子引入细胞质引起的，并使膜蛋白可以自由移动。然后，由IMP（膜内颗粒）聚类形成的裸脂质双层可以直接接触而不会受到IMPS干扰。在附着区域，细胞膜迅速融合。这是，HVJ通过HVJ的细胞融合的基本机制似乎与PEG，电穿孔和显微外科手术的融合机制相似。2。该介绍得到了……更多的发现如下：已经证明，老化的HVJ病毒很容易通过碰撞融合在一起，但是在受感染细胞萌芽后立即将年轻的病毒融合在一起，表现出对这种融合的耐药性。众所周知，由于与M蛋白的联系破坏了3。简单的脂质体可以与融合在一起的细胞融合：据报道，含有白喉毒素片段的脂质体A显示出具有B-淋巴细胞的HIV感染细胞的选择性杀死的活性。孔子可能会在这些细胞表面暴露于裸脂质双层，并且脂质体能够与该区域融合。4。 F和HANA糖蛋白的cDNA已从HVJ或NDV感染细胞中分离出来。这些含有SV40 ORI的cDNA质粒在COS细胞中介绍时显示出高表达。现在，在LLCMK2，CV1和L细胞中观察到含有这些cDNA和肌动蛋白启动子的质粒的表达。该项目尚未完成。较少的