The role of glias in the chronic spinal cord compression (in reference to neural survival and plasticity)

神经胶质细胞在慢性脊髓压迫中的作用（参考神经存活和可塑性）

基本信息

批准号：
09671480
负责人：
BABA Hisatoshi
金额：
$ 1.41万
依托单位：
Fukui Medical University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1997
资助国家：
日本
起止时间：
1997 至 1998
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09671480/
关键词：
Motoneuron Chronic Compression Spinal Cold Nerve Growth Factor Immunohistochemistry Morphology

项目摘要

We investigated the morphology of spinal accessory motoneurons and immunoreactivity to neurotrophins, brain-derived neurotropic factor (BDNF) and neutotrophin (NT)-3, as well as the presence of reactive astrocytosis in 30 tip-toe walking Yoshimura (twy) mice that develop calcification at C1-C2 vertebral level compressing the spinal cord. At the level of compression, the area of neuronal soma and total length of dendrites of WGA-HRP-labelled accessory motoneurons in the medial cell pool decreased significantly with decrement in motoneuron population, when compared to controls. In contrast, at site rostral to the compressive lesion, enlargement of the neuron soma and dendritic elongation were significant and associated with increased motoneuron population and decreased transverse area of the cord at the compressive level, At this site, enhanced BDNF and NT-3 immunoreactivities were evident in the anterior horn cells. In mice with more severe compression, astrocyte-like cells showing BDNF immunoreactivity became abundant and axons in the anterior column demonstrated marked NT-3 immunoreactivity. Our results suggest increased functional activity of anterior horn cells at levels rostral to the site of compression.We speculate that the presence of BDNF and NT-3 in neurons as well as astrocyte-like cells is proportionate to the severity of chronic mechanical compression and may contribute to the heterotropic neuronal reserve and survival.

我们研究了 30 只踮起脚尖行走的 Yoshimura (twy) 小鼠的脊髓辅助运动神经元的形态和对神经营养因子、脑源性神经营养因子 (BDNF) 和中性营养因子 (NT)-3 的免疫反应性，以及反应性星形细胞增多的存在。 C1-C2 椎骨水平钙化压迫脊髓。在压缩水平，与对照组相比，内侧细胞池中 WGA-HRP 标记的辅助运动神经元的神经元胞体面积和树突总长度随着运动神经元群体的减少而显着减少。相比之下，在压缩性病变的喙部，神经元胞体增大和树突伸长显着，并且与压缩水平的运动神经元数量增加和脊髓横向面积减少相关。在该位置，BDNF 和 NT-3 免疫反应性增强前角细胞明显。在受到更严重压迫的小鼠中，显示 BDNF 免疫反应性的星形胶质细胞样细胞变得丰富，并且前柱中的轴突表现出明显的 NT-3 免疫反应性。我们的结果表明，受压部位吻侧水平的前角细胞的功能活性增加。我们推测，神经元以及星形胶质细胞样细胞中 BDNF 和 NT-3 的存在与慢性机械压迫的严重程度成正比，并且可能有助于异向性神经元储备和存活。