Blood damage and turbulence: in vitro study of the turbulence-induced hemolysis in a Taylor-Couette-System

血液损伤和湍流：Taylor-Couette 系统中湍流引起的溶血的体外研究

• 批准号: 507267166
• 负责人: Professor Dr.-Ing. Leonid Goubergrits
• 金额: --
• 依托单位: Institut für Kardiovaskuläre Computer-assistierte Medizin (CVK)
• 依托单位国家: 德国
• 项目类别: Research Grants
• 资助国家: 德国
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/507267166?language=en
• 关键词: Blood; damage; turbulence; vitro; study

Blood damage caused by turbulence is a huge problem in the clinical use of cardiovascular implants such as ventricular assist devices. Objective: The objective of the project is to investigate the blood damage caused by turbulence in-vitro. This has not yet been achieved, as turbulence chambers commonly used in fluid mechanics are not suited for investigation of blood flows. Using an experimental turbulence chamber, a Taylor-Couette system (TCS) with counter-rotating cylinders, turbulence can be generated, and blood damage caused by turbulent flow features can be investigated experimentally. State of the art: The importance of blood damage for ventricular assist devices is judged as an important issue in the international literature. A variety of damage models is presented, but there is a lack of consensus between the respective models as well as between models and underlying experiments. Currently, there is no in-vitro model known, which permits to subject a limited blood volume to a defined turbulence. Hypothesis: The hypothesis is, that blood damage caused by turbulence can be experimentally investigated in a special TCS. In this flow chamber the inner and the outer cylinder can be rotated independently from each other. When counter rotating with distinct rotational speeds, featureless turbulence is generated in the gap between both cylinders. So far, this effect was not yet used for the investigation of blood damage due to turbulence flows. Furthermore, a nearly homogenous laminar shear flow with similar average shear rates can be generated in the same Couette flow chamber. Using this feature, a direct comparison between laminar and turbulent flow conditions can be performed. Methods: Using the TCS with counter rotating cylinders, a turbulent flow is generated in the gap. Using the same flow chamber, the fluid in the gap can be subjected to laminar and turbulent shear flow with the same average shear rate. The flow characters are investigated using a test fluid modelling the multiphasic behavior of blood and a two velocity-component LDA probe. Characteristic turbulence parameters are assessed as a function of rotational speed and effects of flow conditions, turbulence intensity, time will be investigated as well as the variance of those turbulent parameters. For the assessment of blood damage, blood samples of animals and healthy volunteers are investigated with current clinical standard methods. Finally, a model for prediction of blood damage based on turbulence parameters will be developed.

湍流引起的血液损害是心血管植入物（例如心室辅助装置）的临床使用的巨大问题。目的：该项目的目的是研究由湍流引起的血液损害。尚未实现这一目标，因为在流体力学中常用的湍流室不适合研究血流。使用实验性湍流室，可以通过实验研究具有反向旋转缸，湍流的泰勒 - 库特系统（TC），可以产生湍流，并可以通过实验研究由湍流引起的血液损伤。最新技术：在国际文献中认为血液损害对心室辅助设备的重要性被认为是重要的问题。提出了多种损害模型，但是各个模型以及模型和基础实验之间缺乏共识。当前，尚无通知模型，该模型允许将有限的血容量降低到定义的湍流中。假设：假设是，可以在特殊的TC中实验研究由湍流造成的血液损伤。在此流动室中，内部圆柱体可以彼此独立旋转。当以不同的旋转速度旋转时，两个气缸之间的缝隙会产生无特征的湍流。到目前为止，由于湍流流动引起的血液损害尚未使用这种作用。此外，可以在同一COUETTE流动室中产生几乎均匀的层状剪切流，具有相似的平均剪切速率。使用此功能，可以进行层流和湍流条件之间的直接比较。方法：使用与反旋转缸的TCS，间隙中产生湍流。使用相同的流室，间隙中的流体可以以相同的平均剪切速率进行层流和湍流剪切流。使用测试液对血液的多相行为和两个速度分量LDA探针进行研究。特征性湍流参数是根据旋转速度和流动条件，湍流强度，时间以及这些湍流参数的方差评估的。为了评估血液损害，使用当前的临床标准方法研究了动物和健康志愿者的血液样本。最后，将开发基于湍流参数预测血液损伤的模型。