Identification of the erythrocyte receptor for Plasmodium yoelii RhopH complex

约氏疟原虫 RhopH 复合物红细胞受体的鉴定

• 批准号: 16017272
• 负责人: TORII Motomi
• 金额: $ 9.6万
• 依托单位: Ehime University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research on Priority Areas
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16017272/
• 关键词: infectious disease; malaria; Plasmodium; マラリア原虫; 赤血球結合蛋白質; レセプター; GPIアンカー

Parasitophorous vacuole formation is a critical step for the successful invasion of host erythrocytes by malaria parasite. Rhoptry proteins are believed to have essential roles in vacuole formation, although their biological roles are poorly understood. To understand the molecular interactions between parasite rhoptry proteins and the erythrocyte during invasion, we have characterized the binding specificity of the high molecular mass rhoptry protein (RhopH) complex to erythrocytes using the rodent malaria parasite, Plasmodium yoelii and proposed that the major erythrocyte receptor for PyRhopH complex is a protein attached to the erythrocyte surface via GPI-anchor.To further characterize and identify the unknown receptor protein, we employed forward genetic analysis using consomic mouse strains derived from C57BL/6J (B6) and MSM/Ms strains based on the difference in the PyRhopH complex binding to the erythrocytes from these mice (erythrocytes from B6 exhibited higher binding than MSM/Ms). These consomic strains have B6 genetic background and a particular chromosome or chromosomal region is replaced with the one from MSM/Ms. A panel of consomic strains available were examined and the binding among consomic strains were found to be not clearly differentiate to B6 type or MSM/Ms type, indicating that multiple factors were involved in the observed binding. However B6-11^<MSM/Ms>, which harbored chromosome 11 derived from MSM/Ms, showed a prominent low binding, even lower than MSM/Ms, thus we considered that the receptor protein potentially located on chromosome 11. Based on this assumption, we are in a process to narrow the candidate region on the chromosome 11 by further linkage analysis between B6 and B6-11^<MSM/Ms>. The GPI-anchored protein on the candidate region would be characterized as a prime candidate for the PyRhopH complex receptor.

寄生泡形成是疟原虫成功侵入宿主红细胞的关键步骤。尽管人们对棒状体蛋白的生物学作用知之甚少，但人们认为其在液泡形成中具有重要作用。为了了解寄生虫棒状体蛋白和红细胞在入侵过程中的分子相互作用，我们利用啮齿类疟原虫约氏疟原虫表征了高分子质量棒状体蛋白（RhopH）复合物与红细胞的结合特异性，并提出了红细胞的主要受体PyRhopH 复合物是一种通过 GPI 锚定附着在红细胞表面的蛋白质。为了进一步表征和鉴定未知的受体蛋白，我们采用使用源自 C57BL/6J (B6) 和 MSM/Ms 品系的康体小鼠品系进行正向遗传分析，基于 PyRhopH 复合物与这些小鼠红细胞结合的差异（来自 B6 的红细胞表现出比 MSM/Ms 更高的结合）。这些染色体株具有 B6 遗传背景，并且特定染色体或染色体区域被 MSM/Ms 的染色体或染色体区域替换。对一组可用的康体菌株进行了检查，发现康体菌株之间的结合没有明显区分为 B6 型或 MSM/Ms 型，这表明观察到的结合涉及多种因素。而B6-11^<MSM/Ms>，其携带来自MSM/Ms的11号染色体，表现出显着的低结合，甚至低于MSM/Ms，因此我们认为该受体蛋白可能位于11号染色体上。假设，我们正在通过进一步分析 B6 和 B6-11^<MSM/Ms> 之间的连锁分析来缩小 11 号染色体上的候选区域。候选区域上的 GPI 锚定蛋白将被定性为 PyRhopH 复合物受体的主要候选蛋白。

项目成果

Potent immunogenicity of DNA vaccines encoding Plasmodium vivax transmission-blocking vaccine candidates Pvs25 and Pvs28-evaluation of

编码间日疟原虫传播阻断候选疫苗 Pvs25 和 Pvs28 的 DNA 疫苗的有效免疫原性-评估

• 发表时间: 2004
• 期刊: Vaccine 22・23-24
• 作者: Kaneko 0;Yim-Lim BYS;Iriko H;Ling IT;Otsuki H;Grainger M;Tsuboi T;Adams JH;Mattei D;Holder AA;Torii M.;Kaneko O et al.;Winter G et al.;Kongkasuriyachai D et al.
• 通讯作者: Kongkasuriyachai D et al.

Apical expression of three RhopHl/Clag proteins as components of the Plasmodium falcioarum RhopH complex.

作为镰状疟原虫 RhopH 复合物成分的三种 RhopH1/Clag 蛋白的顶端表达。

• 发表时间: 2005
• 期刊: Mol Biochem Parasitol 143(1)
• 作者: Kaneko 0;Yim-Lim BYS;Iriko H;Ling IT;Otsuki H;Grainger M;Tsuboi T;Adams JH;Mattei D;Holder AA;Torii M.
• 通讯作者: Torii M.

2-Cys peroxiredoxin TPx-1 is involved in gametocyte development in Plasmodium berghei

• DOI: 10.1016/j.molbiopara.2006.02.018
• 发表时间: 2006-07-01
• 期刊: MOLECULAR AND BIOCHEMICAL PARASITOLOGY
• 影响因子: 1.5
• 作者: Yano, Kazuhiko;Komaki-Yasuda, Kanako;Kawazu, Shin-ichiro
• 通讯作者: Kawazu, Shin-ichiro

Nasal immunization with a malaria transmission-blocking vaccine candidate Pfs25 induces complete protective immunity in mice against field-isolated Plasmodium falciparum

使用阻断疟疾传播的候选疫苗 Pfs25 进行鼻免疫可诱导小鼠针对现场隔离的恶性疟原虫产生完全保护性免疫力

• 发表时间: 2005
• 期刊: Infection and Immunity 73
• 作者: Arakawa T;et. al.
• 通讯作者: et. al.

Apical expression of three RhopH1/Clag proteins as components of th Plasmodium falciparum RhopH complex

作为恶性疟原虫 RhopH 复合物成分的三种 RhopH1/Clag 蛋白的顶端表达

• 发表时间: 2005
• 期刊: Molecular and Biochemical Parasitology 143
• 作者: Kaneko 0;Yim-Lim BYS;Iriko H;Ling IT;Otsuki H;Grainger M;Tsuboi T;Adams JH;Mattei D;Holder AA;Torii M.;Kaneko O et al.
• 通讯作者: Kaneko O et al.