Innovation of antibody enzymes and their application to produce the materials

抗体酶的创新及其在材料生产中的应用

• 批准号: 13125201
• 负责人: KITAZUME Tomoya
• 金额: $ 10.88万
• 依托单位: Tokyo Institute of Technology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research on Priority Areas
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-13125201/
• 关键词: antibody enzyme; selectivity; ionic liquid; Reuse of reaction system; 反応場; 選択性; イオン性液体; フッ素化合物; 再使用可能; アルドラー型抗体酵素; マイケル付加反応; antibody enzyme; selectivity; ionic liquid; Reuse of reaction system; 反応場; 選択性; イオン性液体; フッ素化合物; 再使用可能; アルドラー型抗体酵素; マイケル付加反応

I examined tha aldol reaction of substituted benzaldehyde and acetone derivative in the antibody aldolase 38C2 (Aldrich no 48157-2)-ionic liquid ([bmim][PF_6]) system. After stirring for 14 days at room temperature, starting materials and products were extracted with diethyl ether, and ionic liquid and antibody aldolase 32C2 were recovered. From the results, we have found that the reaction of hydroxyacetone with 4-or 3-(trifluoromethyl) benzaldehyde in this system was proceeded to produce the 3,4-dihydroxy-4-(4-or 3-trifluoromethyl) phenylbutan-2-One. However, acetone, methyl ethyl ketone, methoxyacetone, fluoroacetone and chloroacetone were not leacted in this system. Moreover, in the cases of aliphatic and/or α, β-unsaturated aldehydes, we did not able to proceed the aldol reaction. Successive reuse of the recovered Ab32C2-ionic liquid system and in the same reaction yielded amounts of product as higher than the first cycle in the both cases of 3-and 4-(trifluoromethyl) bezaldehyde. In the third and fourth cycles, reuse of this system recovered from the second cycle is possible to produce the same alcohol in the same reaction.In the next step, I examined the Michael addition reaction of hydroxyacetone to phenetyl 2-trifluoromethyacrylate in the ionic liquid [emim][OTf]-aldolase antibody 38C2 system.In conclusion, the successful application of this combination of environmentally friendly solvent and antibody catalyst is exciting and is likely to open the door to the development of similar reaction systems for other important organic reactions.

我检查了在抗体醛糖酶38c2（Aldrich No 48157-2）中取代的苯甲醛和丙酮衍生物的Tha醛反应。在室温下搅拌14天后，用二乙醚提取起始材料和产物，并回收离子液体和抗体醛糖酶32C2。从结果来看，我们发现该系统中羟基乙酮与4或3-（三氟甲基）苯甲醛的反应是生产3,4-二羟基4-（4-或3-三氟甲基）苯基苯基苯基苯基苯基苯基苯基苯基苯基苯基苯基苯基苯基苯基苯基苯基苯基苯基苯基苯基苯基苯基苯基苯基苯基苯基苯基苯基苯基苯基苯基苯基苯基苯基苯基苯基苯基苯基苯基苯基甲醛。然而，该系统中未侧重，丙酮，甲基酮，丙氧乙酮，氟乙酮和氯乙酮未弯曲。此外，在脂肪族和/或α的情况下，我们无法进行醛醇反应。连续回收的AB32C2离子液体系统和相同反应的重复使用产生的产物量高于第一个3和4-（三氟甲基）甲醛的第一个周期。在第三个和第四个周期中，可以从第二个周期中回收的该系统在同一反应中产生相同的酒精。在下一步中，我检查了迈克尔在离子液体中的迈克尔与2-三氟甲基丙烯酸苯基丙烯酸酯在离子液中的添加反应[EMIM] [EMIM] [OTF] - α剂量388C2的组合。抗体催化剂令人兴奋，很可能为开发其他重要有机反应的类似反应系统的开发打开了大门。