properties of the masticatory muscle primary afferent neurons from rat trigeminal ganglion

大鼠三叉神经节咀嚼肌初级传入神经元的特性

• 批准号: 17591905
• 负责人: MORITANI Masayuki
• 金额: $ 2.05万
• 依托单位: OSAKA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17591905/
• 关键词: trigeminal nerve; masticatory muscle; sensory; primary afferent neuron; rat; nociception; neural tracer; P2X_3 receptor; 三叉神経節; ATP受容体; ニューロペプチド; 一次求心神経

Masticatory muscle pain is one of the important symptoms of the tempolomandibular disorders. In this project, properties of the masticatory muscle primary afferent neurons from the rat trigeminal ganglion were investigated. The distribution and modulation of the P2X_3 receptor was studied to provide insight into the role of ATP in craniofacial sensory mechanisms. Binding to the D-galactose specific lectin IB4 was found in 73% of P2X_3-positive neurons while only 16% of IB4 neurons expressed P2X_3. Neurons expressing P2X_3 alone were significantly larger than IB4-or IB4/P2X_3-positive neurons. Investigation of target-specificity revealed that 22% of trigeminal ganglion muscle afferent neurons were positive for P2X_3 versus 16% of cutaneous afferent neurons. Muscle P2X_3 afferents were significantly smaller than the overall muscle afferent population while P2X_3 cutaneous afferent neurons were not. Presumptive heteromeric (P2X_<2/3>) muscle afferent neurons were also identified and comprised 77% of the P2X_3 muscle afferent population. Muscle afferent neurons co-expressed P2X_3 with either calcitonin gene-related peptide (15%) or substance P (4%). The number of P2X_3-positive muscle afferent neurons significantly increased one and four days following complete Freund's adjuvant-induced masseter muscle inflammation, but significantly decreased after 12 days. These results indicate that within trigeminal ganglia : 1.the P2X_3 receptor is expressed in both small and medium-sized neurons ; 2.the P2X_3 receptor is not exclusively expressed in IB4 neurons ; 3.P2X_3 is co-expressed with neuropeptides ; 4.differences in the proportion of cutaneous versus muscle P2X_3 afferents are not apparent. Trigeminal P2X_3 neurons therefore differ markedly from dorsal root ganglion P2X_3 afferents. This study also shows that deep tissue inflammation modulates expression of the P2X_3 receptor and thus may warrant exploration as a target for therapeutic intervention.

咀嚼性肌肉疼痛是脾脏疾病的重要症状之一。在这个项目中，研究了大鼠三叉神经节的咀嚼肌原发性传入神经元的特性。研究了P2X_3受体的分布和调节，以洞悉ATP在颅面感觉机制中的作用。在73％的P2X_3阳性神经元中发现了与D-半乳糖特异性凝集素IB4的结合，而只有16％的IB4神经元表达P2X_3。单独表达P2X_3的神经元明显大于IB4-or ib4/p2x_3阳性神经元。对靶标特异性的研究表明，三叉神经节肌肉传入神经元的22％对P2X_3呈阳性，而皮肤传入神经元为16％。肌肉p2x_3的传入明显小于总体肌肉传入种群，而p2x_3皮肤传入神经元没有。还鉴定出肌肉传入神经元的推定异源（P2X_ <2/3>），并占P2X_3肌肉传入群的77％。肌肉传入神经元与降钙素基因相关肽（15％）或物质P（4％）共表达P2X_3。完成Freund佐剂引起的咬肌肌肉炎症后，P2X_3阳性传入神经元的数量显着增加了一和四天，但在12天后显着减少。这些结果表明，在三叉神经节内：1。P2X_3受体在中小型神经元中均表达； 2. P2X_3受体不仅在IB4神经元中表达； 3.P2X_3与神经肽共表达； 4.皮肤与肌肉p2x_3的比例差异不明显。因此，三叉神经元的神经元与背根神经节P2X_3传入明显不同。这项研究还表明，深组织炎症调节了P2X_3受体的表达，因此可能需要探索作为治疗干预的靶标。

项目成果

Bilateral projection of functionally characterized trigeminal oralis neurons to trigeminal motoneurons in cats.

猫功能特征三叉神经口神经元向三叉运动神经元的双边投射。

• 发表时间: 2005
• 期刊: Brain Research 1036
• 作者: Ambalavanar R;Moritani M;Moutanni A;Gangula P;Yallampalli C;Dessem D.;Ambalavanar R;Abe T;Yoshida A
• 通讯作者: Yoshida A

Spatial distribution patterns of excitatory and inhibitory synapses in the dendritic tree differ between jaw-closing and -opening motoneurons.

• DOI: 10.1016/j.archoralbio.2006.11.003
• 发表时间: 2007-04
• 期刊: Archives of oral biology
• 影响因子: 3
• 作者: Y. Shigenaga;Y. Bae;M. Moritani;A. Yoshida

Comparisons between excitatory and inhibitory mechanisms on jaw-closing motoneurons.

闭颌运动神经元的兴奋性和抑制性机制的比较。

• 发表时间: 2005
• 期刊: Dentistry in Japan 41
• 作者: Takemura M;Sugiyo S;Moritani M;Kobayashi M;Yonehara N.;Shigenaga Y;Takemura M;Moritani M
• 通讯作者: Moritani M

The distribution of inhibitory and excitatory synapses on single, reconstructed jaw-opening motoneurons in the cat.

猫的单个重建的张口运动神经元上的抑制性和兴奋性突触的分布。

• 发表时间: 2005
• 期刊: Neuroscience 133
• 作者: Takemura M;Sugiyo S;Moritani M;Kobayashi M;Yonehara N.;Shigenaga Y;Takemura M;Moritani M;Ambalavanar R;Bae YC;Shigenaga Y
• 通讯作者: Shigenaga Y