Research on the Factor that Determines Susceptibility to Chemotherapy for Musculoskeletal Sarcoma by Genomic/Proteomic Analysis

基因组/蛋白质组分析决定肌肉骨骼肉瘤化疗敏感性的因素

• 批准号: 17591604
• 负责人: KAWAI Akira
• 金额: $ 2.24万
• 依托单位: National Cancer Center Research Institute and Research Center for Innovative Oncology, National Cancer Center Hospital East
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17591604/
• 关键词: Bone and soft tissue sarcoma; Gene expression analysis; Two dimensional difference gel electrophoresiS; prognostic factor; malignant fibrous histiocytoma; GIST; osteosarcoma; 骨肉腫; 悪性骨軟部腫瘍; 網羅的遺伝子発現解析; フェチン; 抗がん剤感受性

This research is aimed to clarify prognostic factors and factors related to the treatment susceptibility of primary musculoskeletal sarcoma including osteosarcoma, Ewing's sarcoma and gastrointestinal stromal tumor (GIST) using genomic/proteomic analytical methods. A feature of the research was to clarify gene and protein expression that determined the prognosis and susceptibility to treatment exhaustively and integratively by using actual clinical musculoskeletal sarcoma samples. RNA/protein were extracted from frozen samples of various malignant soft tissue sarcomas and GISTs, and exhaustive gene expression analyses were carried out using Affymetrix U133 GeneChip and exhaustive protein expression analyses using fluorescence secondary electrophoresis/mass spectrometry. Based on the results of the exhaustive gene expression analyses of malignant soft tissue sarcomas using GeneChip, we found that so-called malignant fibrous histiocytoma (MFH) could be subclassified into several subtypes … More that suggested a tendency to respectively differentiate into leiomyosarcoma, liposarcoma, etc. depending on the gene expression pattern. We also identified proteins that featured respective histological types of malignant fibrous histiocytoma by exhaustive protein expression analysis, and reported that the expression of tropomyosin subtype was involved in the subclassification of leiomyosarcoma into its subtypes. As a result of exhaustive protein expression analyses of GISTs, we detected a protein that was significantly different in its expression levels between the arm of early relapsers within one year after surgery (poor prognosis), and the arm of disease-free survivals for over two years (good prognosis). The mRNA that encoded this protein demonstrated similar changes in its expression levels also in the GeneChip analysis. This protein is called pfetin, and a significant difference (p<0.0001) was also found between the good prognosis and the poor prognosis arms in the antipfetin antibody immunostaining and Western blotting using GIST surgery samples. We also conducted parallel clinical and clinicopathologic research of primary musculoskeletal sarcoma. Less

这项研究的目的是阐明与原发性肌肉骨骼肉瘤的治疗敏感性有关的预后因素和因素，包括骨肉瘤，尤因的肉瘤和胃肠道间质肿瘤（GIST）使用基因组/蛋白质组学分析方法。该研究的一个特征是阐明基因和蛋白质表达，这些基因和蛋白质表达通过使用实际的临床肌肉骨骼肉瘤样品来确定对治疗的预后和易感性。从各种恶性软组织肉瘤和GIST的冷冻样品中提取RNA/蛋白质，并使用荧光二级电泳/质谱法使用Affymetrix U133 Genechip和详尽的蛋白质表达分析进行了详尽的基因表达分析。基于使用Genechip对恶性软组织肉瘤进行详尽基因表达分析的结果，我们发现所谓的恶性纤维组织组织细胞瘤（MFH）可以将其分类为几种亚型……更多的是，这表明倾向于在liposarcoma，liposarcoma，liposarcoma，ectectional上分别倾向于依赖于Gene的表达。我们还鉴定出通过详尽的蛋白质表达分析具有相对组织学类型的恶性纤维组织细胞瘤的蛋白质，并报告说，troloososin uspype的表达参与了平滑肌肉瘤的亚分类中的亚型。由于详尽的蛋白质表达分析，我们检测到了一种蛋白质，该蛋白在手术后一年（预后不良）和无疾病生存的手臂（良好的预后）在早期继电器之间的表达水平显着差异。编码该蛋白质的mRNA在Genechip分析中也表现出其表达水平的类似变化。该蛋白质称为pfetin，在抗脂蛋白抗体免疫染色和使用GIST手术样品中的良好预后和预后臂之间也存在显着差异（P <0.0001）。我们还对原发性肌肉骨骼肉瘤进行了平行的临床和临床病理研究。较少的

项目成果

Bilateral adrenal gland metastasis from malignant fibrous histiocytoma : Value of [F-18] FDG PET-CT for diagnosis of occult metastases

恶性纤维组织细胞瘤双侧肾上腺转移：[F-18]FDG PET-CT 对隐匿性转移的诊断价值

• 发表时间: 2006
• 期刊: Annals of Nuclear Medicine 20
• 作者: Eisuke Kobayashi;Akira Kawai;Kunihiko Seki;Ukihide Tateishi
• 通讯作者: Ukihide Tateishi

乳癌長管骨骨転移に対する診断と治療

乳腺癌长骨转移的诊断和治疗

• 发表时间: 2005
• 期刊: 整形・災害外科 48
• 作者: 中山ロバート;川井 章
• 通讯作者: 川井 章

整形外科診療実践ガイド

骨科治疗实践指南

• 作者: Misu T;小野寺淳一;Nakashima I.;Lennon VA.;Nakamura M.;Nakashima I.;Nakashima I.;Narikawa K;Matsumoto Y;藤原一男;三須建郎;Nakashima I;藤原一男;斎田孝彦;Fujihara K.;Misu T.;Saida T.;Nakashima I.;Narikawa K;Narikawa K.;Takahashi T.;斎田孝彦;Saida T.;藤原一男
• 通讯作者: 藤原一男

Proteomic signatures corresponding to histological classification and grading of soft-tissue sarcomas

与软组织肉瘤的组织学分类和分级相对应的蛋白质组学特征

• 发表时间: 2006
• 期刊: Proteomics 6
• 作者: Yoshiyuki Suehara;Tadashi Hasegawa;Akira Kawai;etc
• 通讯作者: etc

整形外科診療実践ガイド 骨肉腫

骨肉瘤骨科治疗实践指南

• 发表时间: 2006
• 作者: Akira Kawai;Yasuo Beppu;Hirokazu Nakauma;Fumihiko Nakatani;Osamu Sato;Akira Kawai;Akira Kawai;Minoru Sakuraba;川井 章;川井 章;川井 章
• 通讯作者: 川井 章