Regulation of plant metabolic function mediated by protein S-nitrosylation

蛋白质S-亚硝基化介导的植物代谢功能调节

• 批准号: 17570039
• 负责人: SAKAMOTO Atsushi
• 金额: $ 2.24万
• 依托单位: HIROSHIMA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17570039/
• 关键词: Protein S-nitrosylation; Reactive nitrogen species (RNS); S-nitrosothiol; S-nitrosoglutathione (GSNO); GSNO reductase; Nitrate assimilation; NO signaling; Arabidopsis; S-ニトロソグルタチオン; S-ニトロソ化合物; 無機窒素同化; アラビドプシス

S-Nitrosylation of cysteine residues is a critical redox modification that regulates a broad spectrum of protein functions, and this process involves reactive nitrogen species (RNS) such as S-nitrosothiols (RSNO) that are known to modify the thiol moiety of proteins. This research attempted to examine: (1) the importance of RSNO metabolism in modulating protein S-nitrosylation in higher plants, with special focus on the regulatory role of S-nitrosoglutathione reductase (GSNOR) as a key enzyme determining cellular RSNO levels and the degree of S-nitrosylation; and (2) the possibility that this posttranslational modification may participate in the regulation of certain plant metabolic functions such as nitrogen assimilation.(1) When exposed to RNS such as NO_2, NO donors and nitrate, Arabidopsis plants increased the level of RSNO, a substantial proportion of which was recovered in high-molecular fractions. This suggests the proteinous nature of accumulated RSNO in response to these inorg … More anic nitrogen oxides. Using biotin-switch method, the changes in S-nitrosylated protein levels were monitored and it was found that NO-donor treatments enhanced protein S-nitrosylation in wild-type plants. Overexpression of GSNOR, however, alleviated the extent of this posttranslational modification, with the increased level much lower than that seen in wild-type plants, supporting the importance of this enzyme in modulating protein S-nitrosylation.(2) The physiological consequence of GSNOR overexpression was investigated with respect to the regulatory aspects of nitrogen assimilation. When fed with a single ^<15>N-labeled nitrogen source such as nitrate or NO_2, transgenic plants overexpressing GSNOR incorporated and assimilated more nitrate-and NO_2-derived ^<15>N than did the wild-type plants. Because GSNOR is catalytically not related to the assimilatory process of inroganic nitrogen, these results suggest the possible involvement of GSNOR, and hence S-nitrosylation of proteins, in the regulation of nitrogen assimilation. Less

半胱氨酸残留物的S-亚硝基化是调节广泛蛋白质功能的关键氧化还原修饰，该过程涉及反应性氮种（RN），例如S-硝基硫醇（RSNO），已知可修饰蛋白质的硫醇基团。这项研究试图检查：（1）RSNO代谢在调节较高植物中蛋白S-硝基化的重要性，特别关注S-硝基谷硫硫代降低（GSNOR）的调节作用（GSNOR）作为确定细胞RSNO水平和S-硝基化度的关键酶。 （2）这种翻译后修饰可能参与某些植物代谢功能（例如氮同化）的可能性。（1）当暴露于NO_2，没有供体和硝酸盐等RN时，Arabidopsis植物会增加RSNO的水平，而RSNO的水平增加了高度菌群中的大量恢复。这表明响应于这些Inorg…更多的氮氧化物的RSNO的蛋白质性质。使用生物素切换方法，监测了S-亚硝基化蛋白水平的变化，发现没有含糖治疗增强了野生型植物中蛋白质S-亚硝基化的增强。 Overexpression of GSNOR, however, alleviated the extent of this posttranslational modification, with the increased level much lower than that seen in wild-type plants, Supporting the importance of this enzyme in modulating protein S-nitrosylation.(2) The physical consequence of GSNOR overexpression was investigated with respect to the regulatory aspects of nitrogen assimilation.当用单个 ^<15> N标记的氮源（例如硝酸盐或NO_2）喂食时，过表达GSNOR的转基因植物掺入并吸收了比野生型植物的硝酸盐和NO_2衍生的 ^<15> N。由于GSNOR在催化上与非果氮的同化过程无关，因此这些结果表明GSNOR可能参与，因此蛋白质的S-硝基化在调节氮同化中。较少的

项目成果

TJ1 is an orientation-independent transformation enhancer sequence

TJ1 是方向无关的转化增强子序列

• 发表时间: 2005
• 期刊: Plant Biotechnol 22
• 作者: H.Hokazono;M.Takahashi;A.Sakamoto;H.Morikawa
• 通讯作者: H.Morikawa

Reactive nitrogen metabolism : a novel frontier in plant nitrogen metabolism.

活性氮代谢：植物氮代谢的新前沿。

• 发表时间: 2005
• 期刊: Plant Biotechnology 22
• 作者: J.Shigeto et al.;J.Shigeto et al.;Morikawa et al.;J.Shigeto et al.;Morikawa et al.;M.Kurumata et al.;M.Takahashi et al.;H.Hokazono et al.;M.Takahashi et al.;M.Takahashi et al.;Morikawa et al.;Takahashi et al.;Hokazono et al.;M.Takahashi et al.;A.Sakamoto et al.
• 通讯作者: A.Sakamoto et al.

Focus on Plant Molecular Biology-2 : Biotechnological Approaches to Improve Nitrogen Use Efficiency in Plants (R.P. Singh and P.K. Jaiwal, eds.)

聚焦植物分子生物学-2：提高植物氮利用效率的生物技术方法（R.P. Singh 和 P.K. Jaiwal 编辑）

• 发表时间: 2006
• 作者: J.Shigeto et al.;J.Shigeto et al.;Morikawa et al.;J.Shigeto et al.;Morikawa et al.;M.Kurumata et al.;M.Takahashi et al.;H.Hokazono et al.;M.Takahashi et al.;M.Takahashi et al.;Morikawa et al.;Takahashi et al.;Hokazono et al.;M.Takahashi et al.;A.Sakamoto et al.;A.Nakagawa et al.;A.Nakagawa et al.;M.Takahashi et al.;M.Takahashi et al.;M.Takahashi et al.(分担執筆);M.Takahashi et al.(分担執筆);Morikawa et al.(分担執筆);H.Morikawa et al.(分担執筆)
• 通讯作者: H.Morikawa et al.(分担執筆)

Proceedings of 2005 Annual Meeting of Koregn Society for Plant Biotechnology and Korea Japan Joint Symposium on Platform Technology for Plant Bioproducts.

韩国植物生物技术学会2005年年会暨韩日植物生物产品平台技术联合研讨会论文集。

• 发表时间: 2005
• 作者: J.Shigeto et al.;J.Shigeto et al.;Morikawa et al.;J.Shigeto et al.;Morikawa et al.;M.Kurumata et al.;M.Takahashi et al.;H.Hokazono et al.;M.Takahashi et al.;M.Takahashi et al.;Morikawa et al.;Takahashi et al.;Hokazono et al.;M.Takahashi et al.;A.Sakamoto et al.;A.Nakagawa et al.;A.Nakagawa et al.;M.Takahashi et al.;M.Takahashi et al.;M.Takahashi et al.(分担執筆);M.Takahashi et al.(分担執筆);Morikawa et al.(分担執筆);H.Morikawa et al.(分担執筆);Morikawa et al.(分担執筆);A.Sakamoto et al.(分担執筆)
• 通讯作者: A.Sakamoto et al.(分担執筆)

Phytoremediators from abandoned rice field.

来自废弃稻田的植物修复剂。

• 发表时间: 2005
• 期刊: Plant Biotechnology 22
• 作者: Takahashi et al.;Morikawa et al.;Morikawa et al.;Kurumata et al.;Takahashi et al.
• 通讯作者: Takahashi et al.