Study on the imaging technique of fluorescent marker and reporter to extract biological function and gene expression base on the ultrasound tagging fluorescence detection method

基于超声标记荧光检测方法的荧光标记和报告基因成像技术提取生物功能和基因表达的研究

基本信息

批准号：
17500314
负责人：
KOBAYASHI Masaki
金额：
$ 2.11万
依托单位：
Tohoku Institute of Technology
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2005
资助国家：
日本
起止时间：
2005 至 2006
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17500314/
关键词：
biomedical imaging fluorescence ultrasound acousto-optic gene expression reporter gene visualization imaging

项目摘要

We have studied on the development of tomographic imaging technique of fluorescence in biological tissue for assays of biological function. Ultrasonic modulation of light based on the acousto-optic effect (so called ultrasound 'tagging') is applied for imaging of fluorescence distribution in the light-scattering media. Sound-field characteristics that affect the light by modulating its amplitude through variation of the refractive index in the medium were determined. With using focused ultrasound, selectively modulated fluorescence on a depth-axis of the medium can be detected. Ultrasound tagging technique applied measuring the optical absorption in light scattering media is well known, and it is principally based on the modulation of speckle pattern. On the contrary, in the case of fluorescence, displacement of scattering particles and variation of the refractive index that is induced by density distribution in a sound field might produce the intensity modulation of scattered light. We have experimentally shown that ultrasound tagging technique is also available for fluorescence measurement. In this paper, we demonstrate the result of tomographic images of fluorescence in dense scattering media using biological tissue, porcine muscle and bovine adipose. Tissue samples had the dimension of 40 × 40 mm in section and fluorescent region which had the 3mm size was embedded in the center of the tissue. The image of the fluorophore was determined with the spatial resolution of focus size of the ultrasound, suggesting the applicability of this technique for visualization of fluorescent probes in deep portion of living body for clinical application. We continue the research aiming to development of a novel diagnostic imaging system.

我们研究了用于生物功能测定的生物组织荧光断层成像技术的发展，基于声光效应的光超声调制（所谓的超声“标记”）应用于光中荧光分布的成像。 - 散射介质。通过介质中折射率的变化来调制光的振幅，从而确定影响光的声场特性。通过使用聚焦超声波，可以检测介质深度轴上的选择性调制荧光。用于测量光散射介质中的光吸收的超声波标记技术是众所周知的，并且其主要基于散斑图案的调制，相反，在荧光的情况下，散射颗粒的位移和折射率的变化。由声场中的密度分布引起的可能会产生散射光的强度调制我们已经通过实验证明超声标记技术也可用于荧光测量。在本文中，我们展示了使用密集散射介质中的荧光的断层扫描图像的结果。生物组织、猪肌肉和牛脂肪的切片尺寸为40×40mm，3mm大小的荧光区域嵌入组织的中心，通过焦点的空间分辨率确定荧光团的图像。超声波的大小，表明该技术在活体深层可视化荧光探针的临床应用中的适用性。我们继续研究，旨在开发一种新型的诊断成像系统。