Study on the imaging technique of fluorescent marker and reporter to extract biological function and gene expression base on the ultrasound tagging fluorescence detection method

基于超声标记荧光检测方法的荧光标记和报告基因成像技术提取生物功能和基因表达的研究

基本信息

批准号：
17500314
负责人：
KOBAYASHI Masaki
金额：
$ 2.11万
依托单位：
Tohoku Institute of Technology
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2005
资助国家：
日本
起止时间：
2005 至 2006
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17500314/
关键词：
biomedical imaging fluorescence ultrasound acousto-optic gene expression reporter gene visualization imaging

项目摘要

We have studied on the development of tomographic imaging technique of fluorescence in biological tissue for assays of biological function. Ultrasonic modulation of light based on the acousto-optic effect (so called ultrasound 'tagging') is applied for imaging of fluorescence distribution in the light-scattering media. Sound-field characteristics that affect the light by modulating its amplitude through variation of the refractive index in the medium were determined. With using focused ultrasound, selectively modulated fluorescence on a depth-axis of the medium can be detected. Ultrasound tagging technique applied measuring the optical absorption in light scattering media is well known, and it is principally based on the modulation of speckle pattern. On the contrary, in the case of fluorescence, displacement of scattering particles and variation of the refractive index that is induced by density distribution in a sound field might produce the intensity modulation of scattered light. We have experimentally shown that ultrasound tagging technique is also available for fluorescence measurement. In this paper, we demonstrate the result of tomographic images of fluorescence in dense scattering media using biological tissue, porcine muscle and bovine adipose. Tissue samples had the dimension of 40 × 40 mm in section and fluorescent region which had the 3mm size was embedded in the center of the tissue. The image of the fluorophore was determined with the spatial resolution of focus size of the ultrasound, suggesting the applicability of this technique for visualization of fluorescent probes in deep portion of living body for clinical application. We continue the research aiming to development of a novel diagnostic imaging system.

我们已经研究了生物组织中荧光层析成像技术的开发，以进行生物学功能测定。基于声学效应（所谓的超声“标记”）的光的超声调制用于光散射介质中荧光分布的成像。通过确定介质中折射率的变化来调节其放大器，从而通过调节其放大器来影响光场特征。使用聚焦超声，可以检测到培养基深度轴上有选择性调节荧光。超声标记技术应用了测量光散射介质中光滥用的技术，并且主要基于斑点模式的调制。相反，在荧光的情况下，散射颗粒的位移以及由声场中密度分布引起的折射率的变化可能会产生散射光的强度调节。我们经过实验表明，超声标记技术也可用于荧光测量。在本文中，我们证明了使用生物组织，猪肌肉和牛脂肪在致密散射培养基中荧光的层析成像图像的结果。组织样品在截面中的尺寸为40×40 mm，荧光区域的尺寸为3mm，嵌入了组织中心。荧光团的图像是通过超声的空间分辨率来确定的，这表明该技术可在生物体内深处可视化荧光问题以在临床应用中可视化荧光问题。我们继续研究旨在开发新型诊断成像系统。