Objective measurement of patch test reactions due to dental metal compounds by measuring the erythema index and quantity of corneous moisture

通过测量红斑指数和角质水分含量来客观测量牙科金属化合物引起的斑贴试验反应

基本信息

批准号：
16592021
负责人：
TAKAHASHI Shinichi
金额：
$ 0.7万
依托单位：
Tokyo Dental College
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2004
资助国家：
日本
起止时间：
2004 至 2005
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16592021/
关键词：
dental metal allergy patch test eryhtema index quantity of corneous moisture

项目摘要

Using the mice delayed hypersensitivity to dinitrochlorobenzene model, we compared the ear swelling rate with erythema index (E.I. ; measured with MEXAMETER MX18【○!R】) and quantity of corneous moisture (Q.G.M. ; measured with CORNEOMETER CM825【○!R】). The ear swelling rate showed a highly significant positive correlation with E.I. but not with Q.C.M. Therefore, E.I was employed for the objective assessment of patch test reactions.To establish the optimal method for this assessment, we measured the effects of measurement intervals and tape application on E.I. during the patch test reactions in normal volunteers. At the site of metal compound and tape application, E.I. was significantly higher within 30 min after tape removal than after >40 min. E.I. was significantly higher on the 2nd,3rd, and 7th day post-patch test than before the patch test. We concluded that to eliminate the effects of tape application on E.I. most effectively, the standard deviation value (S.D.V) of E.I. at the test site should be preferred over E.I. at the control sites (sites of adjacent tape application and the adjacent normal skin).We measured the S.D.V. of E.I. in patients with metal allergy (n=10), in those with an irritant reaction (n=10), and in the control group (n=10) on the 2nd,3rd, and 7th day post-patch test. The S.D.V. of E.I. was significantly higher in patients with metal allergy than in the control group on all days examined. S.D.V. was higher in patients with irritant reactions than in the control group on the 2nd and 3rd day, but not on the 7th day. Only on the 7th day, patients with metal allergy had a higher S.V.D. than those with an irritant reaction. These results suggested that patch test reactions can be objectively assessed with MEXAMETER by employing consecutive measurements.

使用小鼠延迟对二硝基氯苯模型的超敏反应，我们将耳朵肿胀率与红斑指数（E.I。;用Mexameter MX18 [○！r]测量）和角色湿度的数量进行了比较（q.g.m。;用角质仪CM825 [Q.G.M。;耳朵肿胀率与E.I.表现出高度显着的正相关。但不与Q.C.M.因此，使用E.I进行了斑块测试反应的客观评估。为了确定该评估的最佳方法，我们测量了测量间隔和磁带应用对E.I的影响。在正常志愿者中的补丁测试反应中。在金属化合物和胶带应用的位置，E.I。磁带拆除后30分钟内比> 40分钟之后要高得多。 E.I.在第二天，第3天和第7天的测试中，比贴片测试之前要高得多。我们得出的结论是，消除了磁带应用对E.I.的影响。最有效的是E.I.的标准出发价值（S.D.V）在测试地点应该优于E.I.在控制位点（相邻胶带应用和相邻的正常皮肤的位置）。我们测量了S.D.V. E.I.在具有刺激性反应的患者（n = 10）的金属过敏患者中（n = 10），在对照组（n = 10）的第二天，第3天和第7天在斑点测试中。 S.D.V. E.I.金属过敏的患者的所有日期都显着高于对照组。 S.D.V.刺激性反应的患者比对照组的患者在第2天和第三天的患者高。仅在第七天，金属过敏的患者才有更高的S.V.D.比有刺激性反应的人。这些结果表明，可以通过使用连续测量来客观地评估补丁测试反应。