Detection of disease-specific marker of multiple sclerosis : Value of antibodies against RNPA2/B1 in cerebrospinal fluid

多发性硬化症疾病特异性标记物的检测：脑脊液中 RNPA2/B1 抗体的价值

• 批准号: 16590833
• 负责人: KURODA Yasuo
• 金额: $ 2.18万
• 依托单位: Saga University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16590833/
• 关键词: multiple sclerosis; ribonucleoproteins; hnRNP B1; HTLV-I-associated myelopathy; autoimmune disease; cerebrospinal fluid; hnRNP A1; 自己抗体; 抗hnRNP抗体; 疾患マーカー; 多発性硬化; 自己免疫性中枢神経疾患; 疾患特異的マーカー; 髄液; 抗hnRNP-A2抗体; multiple sclerosis; ribonucleoproteins; hnRNP B1; HTLV-I-associated myelopathy; autoimmune disease; cerebrospinal fluid; hnRNP A1; 自己抗体; 抗hnRNP抗体; 疾患マーカー; 多発性硬化; 自己免疫性中枢神経疾患; 疾患特異的マーカー; 髄液; 抗hnRNP-A2抗体

In order to clarify the pathognomic value of antibodies (Abs) against heterogeneous nuclear ribonucleoprotein (hnRNP) A2/B1 in multiple sclerosis (MS), we assayed anti-hnRNP A1 and A2/B1 Abs in 168 cerebrospinal fluid (CSF) samples in fully masked condition. We used recombinant hn RNP A1 and B1 proteins and Western Immunoblots for the assay. The decoding of CSF samples showed that all were obtained from patients with neurological symptoms tested for the infection with human T-lymphotropic virus type I (HTLV-I). Among the 102 cases with negative anti-HTLV-I Abs, the incidence of CSF anti-hnRNP A2/B1 Abs was the highest in MS group (88.0%, n=25), that was significantly different from the incidence in the group of neurodegenerative diseases (15.5%, n=20) (p<0.0001, that of meningoencephalitis (38.1%, n=21)(p=0.0013) and that of inflammatory polyneuropathies (36.8%, n=19)(p=0.0013). Among the 66 cases with positive anti-HTLV-I Abs, the incidence of CSF anti-hnRNP A1 Abs were far lower in HTLV-!-associated myelopathy (HAM)(35.0%, n=40) than in the group of other neurological diseases (57.7%, n=26). The present study confirmed our previous finding showing CSF anti-hnRNP A2/B1 Abs to become a biomarker in the diagnosis of MS, but it did not support the association between anti-hnRNP A1 and HAM.

为了澄清多发性硬化症（MS）中异质性核核核糖蛋白（HNRNP）A2/B1的抗病值（ABS），我们在168个脑脊液（CSF）样品中测定了168个抗脑脊液（CSF）的抗HNRNP A1和A2/B1 ABS。我们使用重组HN RNP A1和B1蛋白和西部免疫印迹进行测定。 CSF样品的解码显示，所有这些都是从患有神经系统症状的患者中获得的，该患者因人类T淋巴细胞病毒I型（HTLV-I）的感染。在102例抗HTLV-I ABS的102例病例中，CSF抗HNRNP A2/B1 ABS的发生率是MS组中最高的（88.0％，n = 25），这与神经退行性疾病的发生率有显着差异n = 21）（p = 0.0013）和炎症性多发性病变（36.8％，n = 19）（p = 0.0013）。在66例抗HTLV-i ABS的66例中，CSF抗HNRNP A1的发生率在HTLV-！ - 近距离肌abs neelopathy neelopathy intery and = 40％（35.0％）中（35.0％）疾病（57.7％，n = 26）证实了我们先前的发现，表明CSF抗HNRNP A2/B1 ABS成为MS诊断的生物标志物，但它不支持抗HNRNP A1和HAM之间的关联。