Phosphorylation of heat shock transcription factor in the stress response

应激反应中热休克转录因子的磷酸化

• 批准号: 16570142
• 负责人: SAKURAI Hiroshi
• 金额: $ 2.3万
• 依托单位: Kanazawa University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16570142/
• 关键词: stress response; heat shock transcription factor; phosphorylation; yeast

All organisms respond to elevated temperatures by changing transcription programs and expressing a set of proteins called heat shock proteins (HSPs). In eukaryotes, heat sock transcription factor (HSF) activates transcription of HSP genes. The target genes of HSF contain a cis-acting sequence designated the heat shock element. The baker's yeast, Saccharomyces cerevisiae, has one HSF encoded by the HSF1 locus. Under heat shock condition, Hsfl protein is extensively phosphorylated and acquires a stronger activating ability, although detailed regulatory mechanisms remain unknown. In this project, I focused on mechanisms of Hsfl phosphorylation and dephosphorylation' and 'roles of Hsfl in the response to various stresses'.By using DNA microarray analysis, we have shown that yeast Hsfl is necessary for heat-induced transcription of〜70 genes and that target genes encode molecular chaperones and proteins involved in a broad range biological functions. We have characterized multicopy suppressor genes of a temperature-sensitive hsfl mutation and found that Hsfl in concert with a protein kinase Pkc1 regulates cell wall remodeling in response to heat shock. Heat-induced hyperphosphorylation of Hsfl was required for transcriptional activation of approximately half of Hsfl target genes. This requirement was related to cooperative interactions among Hsfl trimers bound to the HSE. Consistent with this, oligomerization of Hsfl was prerequisite for stress-induced hyperphosphorylation of Hsfl. We have also shown that human HSF1 recognizes HSEs in a slightly different way than yeast Hsfl.Taken together, these results suggest that the architecture of the HSE is an important determinant for HSF-HSE interactions

所有生物都对升高的转录程序做出了反应，并表达芳烃，热袜子转录因子（HSF）激活了HSP Gens的转录。 HSF由HSF1基因座编码。 70个基因和靶基因都涉及广泛的生物学功能。与HSE的HSFL三聚体之间的目标，与HSE相一致。是HSF-HSE相互作用的重要决定因素

项目成果

熱ショック転写因子によるストレス応答の制御

热休克转录因子控制应激反应

• 发表时间: 2005
• 期刊: 生化学 77・4
• 作者: Naoya Hashikawa;Yu Mizukami;Hiromi Imazu;Hiroshi Sakurai;桜井 博
• 通讯作者: 桜井 博

The DNA-binding domain of yeast Hsf1 regulates both DNA binding and transcriptional activities.

酵母 Hsf1 的 DNA 结合域调节 DNA 结合和转录活性。

• 发表时间: 2006
• 期刊: Biochemical and Biophysical Research Communications 346・4
• 作者: Yamamoto;A.;Sakurai H.
• 通讯作者: Sakurai H.

Phosphorylation of the yeast heat shock transcription factor is implicated in gene-specific activation dependent on the architecture of the heat shock element

• DOI: 10.1128/mcb.24.9.3648-3659.2004
• 发表时间: 2004-05-01
• 期刊: MOLECULAR AND CELLULAR BIOLOGY
• 影响因子: 5.3
• 作者: Hashikawa, N;Sakurai, H
• 通讯作者: Sakurai, H

The DNA-binding domain of yeast Hsfl regulates both DNA binding and transcriptional activities.

酵母 Hsfl 的 DNA 结合域调节 DNA 结合和转录活性。

• 发表时间: 2006
• 期刊: Biochemical and Biophysical Research Communications 346・4
• 作者: Yamamoto;A.;Sakurai H.
• 通讯作者: Sakurai H.

Mutated yeast heat shock transcription factor activates transcription independently of hyperphosphorylation

• DOI: 10.1074/jbc.m510827200
• 发表时间: 2006-02-17
• 期刊: JOURNAL OF BIOLOGICAL CHEMISTRY
• 影响因子: 4.8
• 作者: Hashikawa, N;Mizukami, Y;Sakurai, H
• 通讯作者: Sakurai, H